scholarly journals In vitro corrosion studies of stainless-steel dental substrates during Porphyromonas gingivalis biofilm growth in artificial saliva solutions: providing insights into the role of resident oral bacterium

RSC Advances ◽  
2020 ◽  
Vol 10 (52) ◽  
pp. 31280-31294
Author(s):  
Ubong Eduok ◽  
Jerzy Szpunar
Keyword(s):  
Stainless Steel ◽  
Porphyromonas Gingivalis ◽  
Artificial Saliva ◽  
Biofilm Growth ◽  
Corrosion Studies ◽  
Oral Bacterium ◽  
Culture Suspension

A stainless-steel 321 dental substrate significantly corroded within Porphyromonas gingivalis growth culture in artificial saliva culture suspension, with and without NaF additive.

scholarly journals Porphyromonas gingivalis lipopolysaccharide stimulation in human periodontal ligament stem cells: role of epigenetic modifications to the inflammation

2017 ◽  
Vol 61 (3) ◽  
Author(s):  
Francesca Diomede ◽  
Soundara Rajan Thangavelu ◽  
Ilaria Merciaro ◽  
Monica D'Orazio ◽  
Placido Bramanti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Porphyromonas Gingivalis ◽  
Inflammatory Disease ◽  
Periodontal Ligament ◽  
Model System ◽  
Epigenetic Mechanism ◽  
Periodontal Ligament Stem Cells ◽  
Porphyromonas Gingivalis Lipopolysaccharide

<p>Periodontitis is a chronic oral inflammatory disease produced by bacteria. Gingival retraction and bone and connective tissues resorption are the hallmarks of this disease. Chronic periodontitis may contribute to the risk of onset or progression of neuroinflammatory pathological conditions, such as Alzheimer’s disease. The main goal of the present study was to investigate if the role of epigenetic modulations is involved in periodontitis using human periodontal ligament stem cells (hPDLSCs) as an <em>in vitro</em> model system. hPDLSCs were treated with lipopolysaccharide of <em>Porphyromonas gingivalis</em> and the expression of proteins associated with DNA methylation and histone acetylation, such as DNMT1 and p300, respectively, and inflammatory transcription factor NF-kB, were examined. Immunofluorescence, Western blot and next generation sequencing results demonstrated that <em>P. gingivalis </em>lipopolysaccharide significantly reduced DNA methylase DNMT1, while it markedly upregulated the level of histone acetyltransferase p300 and NF-kB in hPDLSCs. Our results showed that <em>P. gingivalis </em>lipopolysaccharide markedly regulate the genes involved in epigenetic mechanism, which may result in inflammation induction. We propose that <em>P. gingivalis </em>lipopolysaccharide-treated hPDLSCs could be a potential in vitro model system to study epigenetics modulations associated with periodontitis, which might be helpful to identify novel biomarkers linked to this oral inflammatory disease.</p>

scholarly journals Fretting and Fretting Corrosion Processes of Ti6Al4V Implant Alloy in Simulated Oral Cavity Environment

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1561
Author(s):  
Marcin Klekotka ◽  
Jan Ryszard Dąbrowski ◽  
Katarzyna Rećko
Keyword(s):  
Artificial Saliva ◽  
Open Circuit ◽  
Fretting Wear ◽  
Dissipated Energy ◽  
Titanium Oxides ◽  
X Ray Diffraction ◽  
Fretting Corrosion ◽  
Pin On Disc

The paper presents the results of in vitro studies of fretting and fretting corrosion processes of Ti6Al4V implant alloy in the environment of natural saliva and self-made mucin-based artificial saliva solutions. The study was performed on a specially designed fretting pin-on-disc tester, which was combined with a set used for electrochemical research. The open circuit potential measurements and potentiodynamic method were used for corrosion tests. The worn surfaces were subjected to microscopic observations and an evaluation of wear. Results were interpreted using the dissipated energy and third-body approaches. The X-ray diffraction analysis showed that titanium oxides constitute over 80% of the friction products. Special attention was paid to the role of saliva and its substitutes, which in certain cases can lead to the intensification of fretting wear. On the basis of the received results, a new phenomenological model of fretting corrosion processes was proposed. This model involves the formation of an abrasive paste that is a combination of metal oxides and the organic components of saliva.

In Vitro Invasion and Survival of Porphyromonas gingivalis in Gingival Fibroblasts; Role of the Capsule

2012 ◽  
Vol 60 (6) ◽  
pp. 469-476 ◽  
Author(s):  
Muhammad Irshad ◽  
Wil A. van der Reijden ◽  
Wim Crielaard ◽  
Marja L. Laine
Keyword(s):  
Porphyromonas Gingivalis ◽  
Gingival Fibroblasts ◽  
In Vitro Invasion

scholarly journals Mutualistic Biofilm Communities Develop with Porphyromonas gingivalis and Initial, Early, and Late Colonizers of Enamel

2009 ◽  
Vol 191 (22) ◽  
pp. 6804-6811 ◽  
Author(s):  
Saravanan Periasamy ◽  
Paul E. Kolenbrander
Keyword(s):  
Porphyromonas Gingivalis ◽  
Dental Plaque ◽  
Fusobacterium Nucleatum ◽  
Single Species ◽  
Biofilm Growth ◽  
Flow Cells ◽  
Tooth Cleaning ◽  
Plaque Development ◽  
Species Specific

ABSTRACT Porphyromonas gingivalis is present in dental plaque as early as 4 h after tooth cleaning, but it is also associated with periodontal disease, a late-developing event in the microbial successions that characterize daily plaque development. We report here that P. gingivalis ATCC 33277 is remarkable in its ability to interact with a variety of initial, early, middle, and late colonizers growing solely on saliva. Integration of P. gingivalis into multispecies communities was investigated by using two in vitro biofilm models. In flow cells, bacterial growth was quantified using fluorescently conjugated antibodies against each species, and static biofilm growth on saliva-submerged polystyrene pegs was analyzed by quantitative real-time PCR using species-specific primers. P. gingivalis could not grow as a single species or together with initial colonizer Streptococcus oralis but showed mutualistic growth when paired with two other initial colonizers, Streptococcus gordonii and Actinomyces oris, as well as with Veillonella sp. (early colonizer), Fusobacterium nucleatum (middle colonizer), and Aggregatibacter actinomycetemcomitans (late colonizer). In three-species flow cells, P. gingivalis grew with Veillonella sp. and A. actinomycetemcomitans but not with S. oralis and A. actinomycetemcomitans. Also, it grew with Veillonella sp. and F. nucleatum but not with S. oralis and F. nucleatum, indicating that P. gingivalis and S. oralis are not compatible. However, P. gingivalis grew in combination with S. gordonii and S. oralis, demonstrating its ability to overcome the incompatibility when cultured with a second initially colonizing species. Collectively, these data help explain the observed presence of P. gingivalis at all stages of dental plaque development.

scholarly journals CYTOTOXICITY OF IONS RELEASED FROM 17-4 PRECIPITATION HARDENING STAINLESS STEEL ORTHODONTIC BRACKETS IN ARTIFICIAL SALIVA

2018 ◽  
Vol 9 ◽  
pp. 71
Author(s):  
Tjokro Prasetyadi ◽  
Bambang Irawan ◽  
Miesje Karmiati Purwanegara ◽  
Bambang Suharno ◽  
Sugeng Supriadi
Keyword(s):  
Stainless Steel ◽  
Cell Viability ◽  
Precipitation Hardening ◽  
Artificial Saliva ◽  
Nickel Content ◽  
Orthodontic Brackets ◽  
Assay Method ◽  
Cytotoxicity Test ◽  
Significant Difference

Objective: 17-4 precipitation hardening (PH) stainless steel has a low nickel content, which can reduce the risk of allergic reactions. It also has good mechanical properties against the stress caused by the archwire slot brackets in orthodontic treatments. The main focus of this study to evaluate the metal ions released into artificial saliva from different orthodontic brackets with the same 17-4 PH stainless steel and to examine the in vitro cytotoxicity of the metal.Methods: Material properties were analyzed by energy dispersive spectroscopy. The 3-(4,5-dimethylthiazol-2-y1)2,5-diphenyltetrazolium bromide (MTT) assay method was used to examine the cytotoxicity of Gemini and Synergy brackets.Results: The cytotoxicity test on all the orthodontic brackets showed a mean cell viability value above 80% in each immersion group, which means that this material is not cytotoxic to the human immortalized keratinocyte cell line.Conclusions: The results showed cell viability in the extracts of both groups of brackets, and there was no statistically significant difference between the groups (p>0.05).

scholarly journals The Role of Porphyromonas gingivalis Virulence Factors in Periodontitis Immunopathogenesis

2020 ◽  
Vol 23 (1) ◽  
pp. 6-12
Author(s):  
Tienneke Riana Septiwidyati ◽  
Endang Winiati Bachtiar
Keyword(s):  
Immune Responses ◽  
Porphyromonas Gingivalis ◽  
Virulence Factors ◽  
Defense Mechanisms ◽  
Tooth Loss ◽  
Innate Immune ◽  
Not Given ◽  
Innate Immune Responses ◽  
Oral Bacterium

Porphyromonas gingivalis is an anaerobic Gram-negative oral bacterium involved in the pathogenesis of periodontitis. Periodontitis is an infection that is characterized by damage to the supporting tissues of the teeth so that it can cause tooth loss if not given treatment. P. gingivalis locally can invade periodontal tissue and avoid host defense mechanisms. This bacterium has virulence factors which can cause deregulation of innate immune responses and inflammation in the host. The role of P. gingivalis virulence factors such as capsules, fimbriae, lipopolysaccharides, and gingipain in the pathogenesis of periodontitis will be discussed in this paper.

scholarly journals Biofilm Growth By Listeria Monocytogenes On Stainless Steel and Expression of Biofilm-Related Genes Under Stressing Conditions

2021 ◽  
Author(s):  
Danilo Augusto Lopes da Silva ◽  
Rafaela de Melo Tavares ◽  
Anderson Carlos Camargo ◽  
Ricardo Seiti Yamatogi ◽  
Elaine Cristina Pereira De Martinis ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Stress Responses ◽  
Biofilm Formation ◽  
Gene Networks ◽  
Stop Codon ◽  
Regulatory Gene ◽  
Premature Stop Codon ◽  
Biofilm Growth ◽  
Adverse Conditions

Abstract This research was carried out to assess the ability of L. monocytogenes for adhesion and growth in biofilm on stainless steel coupons under different stressing conditions (NaCl, curing salts and quaternary ammonium compounds - QAC), besides determining the expression of different genes involved in biofilm formation and stress response. Results from crystal violet assay revealed that one isolate carrying a premature stop codon (PMSC) in agrC gene formed high-density biofilms in the presence of QAC or cure salts (7.5% and 10%). Reverse Transcriptase-qPCR results revealed that isolates of L. monocytogenes lineages I and II presented differences in transcriptional profile of genes related to biofilm formation and adaptation to environmental conditions. In conclusion, our results demonstrated how L. monocytogenes can survive, multiply and form biofilm under adverse conditions related to food processing environments. Differences in transcriptional expression were observed, highlighting the role of regulatory gene networks for particular serotypes under different stress responses.

scholarly journals Mixed lineage kinase domain-like pseudokinase mediated necroptosis aggravates periodontitis progression

2021 ◽  
Author(s):  
Yanan Yang ◽  
Lingxia Wang ◽  
Haibing Zhang ◽  
Lijun Luo
Keyword(s):  
Bone Marrow ◽  
Bone Loss ◽  
Porphyromonas Gingivalis ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Mrna Levels ◽  
Genetic Ablation ◽  
Osteoclast Activation

Abstract Necroptosis is a form of cell death that is reportedly involved in the pathogenesis of periodontitis. However, the role of Mlkl-involved necroptosis remains unclear. Herein, we aim to explore the role of MLKL-mediated necroptosis in periodontitis in vitro and in vivo. Expression of RIPK3, MLKL, and phosphorylated MLKL is observed in gingival tissues obtained from healthy subjects or patients with periodontitis. Viability of Porphyromonas gingivalis lipopolysaccharide (LPS-Pg)-treated cells was detected. In wild type or Mlkl deficiency mice with ligature-induced periodontitis, alveolar bone loss and osteoclast activation were assessed. mRNA levels of inflammatory cytokines in bone marrow-derived macrophages were tested by qRT-PCR. Increased expression of RIPK3, MLKL, and phosphorylated MLKL is observed in gingival tissues obtained from patients with periodontitis. Porphyromonas gingivalis lipopolysaccharide (LPS-Pg)-treated cells developed necroptosis after caspase inhibition and negatively regulated the NF-κB signaling pathway. In mice with ligature-induced periodontitis, Mlkl deficiency reduced alveolar bone loss and weakened osteoclast activation. Furthermore, genetic ablation of Mlkl in LPS-Pg-treated bone marrow-derived macrophages increased the mRNA levels of tumor necrosis factor-α, interleukin (Il)-1β, Il-6, cyclooxygenase 2, matrix metalloproteinase 9, and receptor activator of nuclear factor kappa-B ligand. Our data indicated that MLKL-mediated necroptosis aggravates the development of periodontitis in a Mlkl-deficient mouse. And this will provide a new sight for the understanding of etiology and therapies of periodontitis.

scholarly journals Coated stainless steel archwires' discoloration measured by computerized system (An in-vitro study)

2020 ◽  
Vol 32 (4) ◽  
pp. 1-4
Author(s):  
Abeer B Mahmood
Keyword(s):  
Stainless Steel ◽  
Color Change ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Chemical Compositions ◽  
Color Changes ◽  
The Aesthetic ◽  
Post Hoc ◽  
Computerized System

Background: Aesthetic archwires are used to overcome the aesthetic problems of stainless steel wires but the color of the coating layer can be changed with time when exposed to oral environments. The aim of this study was to evaluate the degree of color change of different aesthetic archwires from different companies under different coloring solutions. Materials and Methods: One hundred fifty samples of coated archwires from three companies (Highland, G&H and Dany) were immersed in 5 solutions (artificial saliva, turmeric, tea, coffee and Miranda) to evaluate the degree of color changes after 7, 14 and 21 days using visible spectrophotometer. Data were collected and analyzed using one way ANOVA and post hoc Tukey’s tests. Results: Turmeric solution caused high color change than other solutions. Aesthetic archwires from Highland company showed the highest degree of color change than archwires from other companies. Conclusions: Turmeric solution produced more discoloration than other solutions and the effects of these solutions are related to different chemical compositions of those solutions.

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