scholarly journals An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection

RSC Advances ◽  
2020 ◽  
Vol 10 (55) ◽  
pp. 33040-33051
Author(s):  
Jeunice Ida ◽  
Akinori Kuzuya ◽  
Yee Siew Choong ◽  
Theam Soon Lim
Keyword(s):  
Dengue Virus ◽  
Virus Detection ◽  
Dna Sensing ◽  
Reporter System ◽  
G Quadruplex ◽  
P Application

Application of split G-quadruplex as DNAzyme reporter system for DNA sensing.

A method of layer-by-layer gold nanoparticle hybridization in a quartz crystal microbalance DNA sensing system used to detect dengue virus

2009 ◽  
Vol 20 (21) ◽  
pp. 215501 ◽  
Author(s):  
Sz-Hau Chen ◽  
Yao-Chen Chuang ◽  
Yi-Chen Lu ◽  
Hsiu-Chao Lin ◽  
Yun-Liang Yang ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Gold Nanoparticle ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Layer By Layer ◽  
Dna Sensing ◽  
Sensing System

scholarly journals Sandwich-Type DNA Micro-Optode Based on Gold–Latex Spheres Label for Reflectance Dengue Virus Detection

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 1820
Author(s):  
Jeningsih ◽  
Ling Ling Tan ◽  
Alizar Ulianas ◽  
Lee Yook Heng ◽  
Nur-Fadhilah Mazlan ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Limit Of Detection ◽  
Virus Detection ◽  
Average Diameter ◽  
Optical Biosensor ◽  
Clinical Samples ◽  
Sandwich Hybridization ◽  
Latex Spheres ◽  
Sandwich Type ◽  
Thiol Binding

A DNA micro-optode for dengue virus detection was developed based on the sandwich hybridization strategy of DNAs on succinimide-functionalized poly(n-butyl acrylate) (poly(nBA-NAS)) microspheres. Gold nanoparticles (AuNPs) with an average diameter of ~20 nm were synthesized using a centrifugation-based method and adsorbed on the submicrometer-sized polyelectrolyte-coated poly(styrene-co-acrylic acid) (PSA) latex particles via an electrostatic method. The AuNP–latex spheres were attached to the thiolated reporter probe (rDNA) by Au–thiol binding to functionalize as an optical gold–latex–rDNA label. The one-step sandwich hybridization recognition involved a pair of a DNA probe, i.e., capture probe (pDNA), and AuNP–PSA reporter label that flanked the target DNA (complementary DNA (cDNA)). The concentration of dengue virus cDNA was optically transduced by immobilized AuNP–PSA–rDNA conjugates as the DNA micro-optode exhibited a violet hue upon the DNA sandwich hybridization reaction, which could be monitored by a fiber-optic reflectance spectrophotometer at 637 nm. The optical genosensor showed a linear reflectance response over a wide cDNA concentration range from 1.0 × 10−21 M to 1.0 × 10−12 M cDNA (R2 = 0.9807) with a limit of detection (LOD) of 1 × 10−29 M. The DNA biosensor was reusable for three consecutive applications after regeneration with mild sodium hydroxide. The sandwich-type optical biosensor was well validated with a molecular reverse transcription polymerase chain reaction (RT-PCR) technique for screening of dengue virus in clinical samples, e.g., serum, urine, and saliva from dengue virus-infected patients under informed consent.

scholarly journals A versatile reporter system to monitor virus infected cells and its application to dengue virus and SARS-CoV-2

2020 ◽  
Author(s):  
Felix Pahmeier ◽  
Christoper J Neufeldt ◽  
Berati Cerikan ◽  
Vibhu Prasad ◽  
Costantin Pape ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Viral Replication ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Reporter System ◽  
Infected Cells ◽  
Positive Strand Rna

ABSTRACTPositive-strand RNA viruses have been the etiological agents in several major disease outbreaks over the last few decades. Examples of that are flaviviruses, such as dengue virus and Zika virus that cause millions of yearly infections and spread around the globe, and coronaviruses, such as SARS-CoV-2, which is the cause of the current pandemic. The severity of outbreaks caused by these viruses stresses the importance of virology research in determining mechanisms to limit virus spread and to curb disease severity. Such studies require molecular tools to decipher virus-host interactions and to develop effective interventions. Here, we describe the generation and characterization of a reporter system to visualize dengue virus and SARS-CoV-2 replication in live cells. The system is based on viral protease activity causing cleavage and nuclear translocation of an engineered fluorescent protein that is expressed in the infected cells. We show the suitability of the system for live cell imaging and visualization of single infected cells as well as for screening and testing of antiviral compounds. Given the modular building blocks, the system is easy to manipulate and can be adapted to any virus encoding a protease, thus offering a high degree of flexibility.IMPORTANCEReporter systems are useful tools for fast and quantitative visualization of viral replication and spread within a host cell population. Here we describe a reporter system that takes advantage of virus-encoded proteases that are expressed in infected cells to cleave an ER-anchored fluorescent protein fused to a nuclear localization sequence. Upon cleavage, the fluorescent protein translocates to the nucleus, allowing for rapid detection of the infected cells. Using this system, we demonstrate reliable reporting activity for two major human pathogens from the Flaviviridae and the Coronaviridae families: dengue virus and SARS-CoV-2. We apply this reporter system to live cell imaging and use it for proof-of-concept to validate antiviral activity of a nucleoside analogue. This reporter system is not only an invaluable tool for the characterization of viral replication, but also for the discovery and development of antivirals that are urgently needed to halt the spread of these viruses.

LSPR-Based Biosensor for Dengue Virus Detection

2021 ◽  
Author(s):  
Felipe M. F. Teixeira ◽  
Gabriel L. Machado ◽  
Gabriel S. C. Ferreira ◽  
Alice F. Versiani ◽  
Lidia M. Andrade ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Detection

scholarly journals Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection

Talanta ◽  
2019 ◽  
Vol 198 ◽  
pp. 358-370 ◽  
Author(s):  
Nur-Fadhilah Mazlan ◽  
Ling Ling Tan ◽  
Nurul Huda Abd. Karim ◽  
Lee Yook Heng ◽  
Nur Diyana Jamaluddin ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Detection ◽  
Labeling Approach

Fabrication of fluidic-based memristor sensor for dengue virus detection

2017 ◽  
Author(s):  
Nor Shahanim Mohamad Hadis ◽  
Asrulnizam Abd Manaf ◽  
Siti Hawa Ngalim ◽  
Sukreen Hana Herman ◽  
Kazuaki Sawada ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Detection

scholarly journals A novel reporter system for neutralizing and enhancing antibody assay against dengue virus

2014 ◽  
Vol 14 (1) ◽  
pp. 44 ◽  
Author(s):  
Ke-Yu Song ◽  
Hui Zhao ◽  
Zhen-You Jiang ◽  
Xiao-Feng Li ◽  
Yong-Qiang Deng ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Reporter System ◽  
Antibody Assay

scholarly journals Pan-Dengue Virus Detection by PCR for Travelers Returning from the Tropics

2008 ◽  
Vol 46 (9) ◽  
pp. 3104-3106 ◽  
Author(s):  
A. Dumoulin ◽  
H. Marti ◽  
M. Panning ◽  
C. Hatz ◽  
H. H. Hirsch
Keyword(s):  
Dengue Virus ◽  
Virus Detection ◽  
The Tropics
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