scholarly journals Sodium alginate/collagen composite multiscale porous scaffolds containing poly(ε-caprolactone) microspheres fabricated based on additive manufacturing technology

RSC Advances ◽  
2020 ◽  
Vol 10 (64) ◽  
pp. 39241-39250
Author(s):  
Shuifeng Liu ◽  
Da Huang ◽  
Yang Hu ◽  
Jiancheng Zhang ◽  
Bairui Chen ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Additive Manufacturing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Sodium Alginate ◽  
Drug Loading ◽  
Manufacturing Technology ◽  
Porous Scaffolds ◽  
Additive Manufacturing Technology

Biocompatible porous scaffolds with adjustable pore structures, appropriate mechanical properties and drug loading properties are important components of bone tissue engineering.

scholarly journals Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO2 Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1319
Author(s):  
Muhammad Umar Aslam Khan ◽  
Wafa Shamsan Al-Arjan ◽  
Mona Saad Binkadem ◽  
Hassan Mehboob ◽  
Adnan Haider ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Guar Gum ◽  
Porous Scaffolds ◽  
Vitro Assay ◽  
Vitro Analysis ◽  
Nanocomposite Scaffolds

Bone tissue engineering is an advanced field for treatment of fractured bones to restore/regulate biological functions. Biopolymeric/bioceramic-based hybrid nanocomposite scaffolds are potential biomaterials for bone tissue because of biodegradable and biocompatible characteristics. We report synthesis of nanocomposite based on acrylic acid (AAc)/guar gum (GG), nano-hydroxyapatite (HAp NPs), titanium nanoparticles (TiO2 NPs), and optimum graphene oxide (GO) amount via free radical polymerization method. Porous scaffolds were fabricated through freeze-drying technique and coated with silver sulphadiazine. Different techniques were used to investigate functional group, crystal structural properties, morphology/elemental properties, porosity, and mechanical properties of fabricated scaffolds. Results show that increasing amount of TiO2 in combination with optimized GO has improved physicochemical and microstructural properties, mechanical properties (compressive strength (2.96 to 13.31 MPa) and Young’s modulus (39.56 to 300.81 MPa)), and porous properties (pore size (256.11 to 107.42 μm) and porosity (79.97 to 44.32%)). After 150 min, silver sulfadiazine release was found to be ~94.1%. In vitro assay of scaffolds also exhibited promising results against mouse pre-osteoblast (MC3T3-E1) cell lines. Hence, these fabricated scaffolds would be potential biomaterials for bone tissue engineering in biomedical engineering.

scholarly journals In Vitro and In Vivo Characterization of N-Acetyl-L-Cysteine Loaded Beta-Tricalcium Phosphate Scaffolds

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Yong-Seok Jang ◽  
Phonelavanh Manivong ◽  
Yu-Kyoung Kim ◽  
Kyung-Seon Kim ◽  
Sook-Jeong Lee ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Water Soluble ◽  
Tetrazolium Salt ◽  
Porous Scaffolds ◽  
Tissue Engineering Application ◽  
Beta Tricalcium Phosphate

Beta-tricalcium phosphate bioceramics are widely used as bone replacement scaffolds in bone tissue engineering. The purpose of this study is to develop beta-tricalcium phosphate scaffold with the optimum mechanical properties and porosity and to identify the effect of N-acetyl-L-cysteine loaded to beta-tricalcium phosphate scaffold on the enhancement of biocompatibility. The various interconnected porous scaffolds were fabricated using slurries containing various concentrations of beta-tricalcium phosphate and different coating times by replica method using polyurethane foam as a passing material. It was confirmed that the scaffold of 40 w/v% beta-tricalcium phosphate with three coating times had optimum microstructure and mechanical properties for bone tissue engineering application. The various concentration of N-acetyl-L-cysteine was loaded on 40 w/v% beta-tricalcium phosphate scaffold. Scaffold group loaded 5 mM N-acetyl-L-cysteine showed the best viability of MC3T3-E1 preosteoblastic cells in the water-soluble tetrazolium salt assay test.

scholarly journals Reconstruction of Bone Defect Based on the Fusion of Osteoblasts and MSM/HA/PLGA Porous Scaffolds

2020 ◽  
Author(s):  
weiling huo ◽  
Xiaodong Wu ◽  
Yancheng zheng ◽  
Jian Cheng ◽  
Qiang Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Defect ◽  
Drug Loading ◽  
Release Time ◽  
Porous Scaffolds ◽  
Polymer Scaffolds ◽  
Experimental Basis

Reconstruction of bone defect is one of the difficult problems in orthopedic treatment, and bone tissue scaffold implantation is the most promising direction of bone defect reconstruction. In this study, we used the combination of HA (Hydroxyapatite) and PLGA [Poly (lactic-co-glycolic acid)] in the construction of polymer scaffolds, and introduced bioactive MSM (Methyl sulfonyl methane) into polymer scaffolds to prepare porous scaffolds. The osteoblasts, isolated and cultured in vitro, were seeded in the porous scaffolds to construct tissue-engineered scaffolds. Meanwhile, the model of rabbit radius defect was constructed to evaluate the biological aspects of five tissue-engineered scaffolds, which provided experimental basis for the application of the porous scaffolds in bone tissue engineering. The SEM characterization showed the pore size of porous scaffolds was uniform and the porosity was about 90%. The results of contact Angle testing suggested that the hydrophobic porous scaffold surface could effectively promote cell adhesion and cell proliferation, while mechanical property test showed good machinability. The results of drug loading and release efficiency of MSM showed that porous scaffolds could load MSM efficiently and prolong the release time of MSM. In vitro incubation of porous scaffolds and osteoblasts showed that the addition of a small quantity of MSM could promote the infiltration and proliferation of osteoblasts on the porous scaffolds. Similar results were obtained by implanting the tissue-engineered scaffolds, fused with the osteoblasts and MSM/HA/PLGA porous scaffolds, into the rabbit radius defect, which provided experimental basis for the application of the MSM/HA/PLGA porous scaffolds in bone tissue engineering.

scholarly journals Fibers by Electrospinning and Their Emerging Applications in Bone Tissue Engineering

2021 ◽  
Vol 11 (19) ◽  
pp. 9082
Author(s):  
Chuqun Yang ◽  
Qi Shao ◽  
Yulai Han ◽  
Qingxia Liu ◽  
Liang He ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Simple Technique ◽  
Drug Loading ◽  
Main Role ◽  
Loading Capacity ◽  
Mechanical Support

Bone tissue engineering (BTE) is an optimized approach for bone regeneration to overcome the disadvantages of lacking donors. Biocompatibility, biodegradability, simulation of extracellular matrix (ECM), and excellent mechanical properties are essential characteristics of BTE scaffold, sometimes including drug loading capacity. Electrospinning is a simple technique to prepare fibrous scaffolds because of its efficiency, adaptability, and flexible preparation of electrospinning solution. Recent studies about electrospinning in BTE are summarized in this review. First, we summarized various types of polymers used in electrospinning and methods of electrospinning in recent work. Then, we divided them into three parts according to their main role in BTE, (1) ECM simulation, (2) mechanical support, and (3) drug delivery system.

Exploration of gum ghatti-modified porous scaffolds for bone tissue engineering applications

2020 ◽  
Vol 44 (6) ◽  
pp. 2389-2401 ◽  
Author(s):  
J. Anita Lett ◽  
Suresh Sagadevan ◽  
Zohreh Shahnavaz ◽  
Muthiah Bavani Latha ◽  
Karthick Alagarswamy ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Repair ◽  
Porous Scaffolds ◽  
Engineering Applications ◽  
Gum Ghatti ◽  
Hydroxyl Apatite

Taking advantage of the tissue engineering principles, the formed hydroxyl apatite-modified gum ghatti biomaterial with its porous nature, biocompatibility, and efficient mechanical properties can be potential for the bone repair and regeneration.

Fabrication and Properties of Porous Scaffolds of PLA-PEG Biocomposite for Bone Tissue Engineering

2014 ◽  
Vol 789 ◽  
pp. 130-135 ◽  
Author(s):  
Ning Wang ◽  
Yong Ju Zang ◽  
Gui Zhi Ren ◽  
Qi Lin Wu
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tetrazolium Salt ◽  
Porous Scaffolds ◽  
Solvent Casting ◽  
Diphenyltetrazolium Bromide ◽  
Murine Fibroblast ◽  
Particulate Leaching

Porous scaffolds of polylactic acid-polyethylene glycol block copolymers (PLA-PEG) biocomposite were fabricated by solvent casting-particulate leaching method using sodium chloride as the porogen. With the aim of evaluating the influence of porosity on mechanical properties and biocompatibility, three specimens of scaffolds which have different porosity (around 50%, 60%, 70%) were fabricated. Murine fibroblast grew cells (L929) were seeded into PLA-PEG porous biocomposite scaffolds. The tetrazolium salt 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium Bromide (MTT), scanning electron microscopy and confocal microscopy were carried out to characterize cell proliferation and morphology. The composite scaffolds with the porosity of 50% possessed better mechanical properties. All scaffolds support attachment, spreading and proliferation of L929, and the biocompatibility of scaffolds could be improved by increasing the porosity. The fabricated PLA-PEG porous biocomposite scaffolds with good mechanical properties and biocompatibility might be used in bone tissue engineering.

Role of Interfacial Interactions on Mechanical Properties of Biomimetic Composites for Bone Tissue Engineering

2005 ◽  
Vol 898 ◽  
Author(s):  
Devendra Verma ◽  
Rahul Bhowmik ◽  
Bedabibhas Mohanty ◽  
Dinesh R Katti ◽  
Kalpana S Katti
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Response ◽  
Density Ratio ◽  
Interfacial Interactions ◽  
Porous Composites ◽  
Properties Of Composites

AbstractInterfaces play an important role in controlling the mechanical properties of composites. Optimum mechanical strength of scaffolds is of prime importance for bone tissue engineering. In the present work, molecular dynamics simulations and experimental studies have been conducted to study effect of interfacial interactions on mechanical properties of composites for bone replacement. In order to mimic biological processes, hydroxyapatite (HAP) is mineralized in presence of polyacrylic acid (PAAc) (in situ HAP). Further, solid and porous composites of in situ HAP with polycaprolactone (PCL) are made. Mechanical tests of composites of in situ HAP with PAAc have shown improved strain recovery, higher modulus/density ratio and also improved mechanical response in simulated body fluid (SBF). Simulation studies indicate potential for calcium bridging between –COO− of PAAc and surface calcium of HAP. This fact is also supported by infrared spectroscopic studies. PAAc modified surfaces of in situ HAP offer means to control the microstructure and mechanical response of porous composites. Nanoindentation experiments indicate that apatite grown on in situ HAP/PCL composites from SBF has improved elastic modulus and hardness. This work gives insight into the interfacial mechanisms responsible for mechanical response as well as bioactivity in biomaterials.

Hybrid nanostructured hydroxyapatite–chitosan composite scaffold: bioinspired fabrication, mechanical properties and biological properties

2015 ◽  
Vol 3 (23) ◽  
pp. 4679-4689 ◽  
Author(s):  
Ya-Ping Guo ◽  
Jun-Jie Guan ◽  
Jun Yang ◽  
Yang Wang ◽  
Chang-Qing Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Biological Properties ◽  
Chitosan Composite

A bioinspired strategy has been developed to fabricate a hybrid nanostructured hydroxyapatite–chitosan composite scaffold for bone tissue engineering.

scholarly journals Highly porous scaffolds of PEDOT:PSS for bone tissue engineering

2017 ◽  
Vol 62 ◽  
pp. 91-101 ◽  
Author(s):  
Anne Géraldine Guex ◽  
Jennifer L. Puetzer ◽  
Astrid Armgarth ◽  
Elena Littmann ◽  
Eleni Stavrinidou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffolds ◽  
Highly Porous
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