scholarly journals Analytical and preparative separation and isolation of functionalized fullerenes by conventional HPLC stationary phases: method development and column screening

RSC Advances ◽  
2020 ◽  
Vol 10 (33) ◽  
pp. 19211-19218
Author(s):  
Merve Ergun Dönmez ◽  
Helena Grennberg
Keyword(s):  
Method Development ◽  
Stationary Phases ◽  
Hplc Method ◽  
Complex Reaction ◽  
Preparative Separation ◽  
Isolation And Purification ◽  
Functionalized Fullerenes

Isolation and purification of functionalized fullerenes from often complex reaction mixtures is challenging. Here, a simple and efficient HPLC method is presented.

scholarly journals Chromatographic Characterization and Method Development for Determination of Levetiracetam in Saliva: Application to Correlation with Plasma Levels

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Imad I. Hamdan ◽  
Mervat Alsous ◽  
Amira Taher Masri
Keyword(s):  
High Performance ◽  
Method Development ◽  
Stationary Phases ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Therapeutic Drug ◽  
Epileptic Children ◽  
Isocratic Hplc ◽  
Liquid Chromatographic

Levetiracetam (LVT) is a widely used antiepileptic drug (AED). A less invasive sampling method for therapeutic drug monitoring (TDM) would be very useful particularly for children. Saliva has been shown as an adequate sample for TDM of some AEDs. Due to the high hydrophilicity of LVT its separation on common stationary phases is quite a challenge so that previous methods for determination of LVT in saliva employed either gradient high performance liquid chromatographic (HPLC) system or mass spectrometer as a detector. In this study the retention behavior of LVT on some common stationary phases was examined, with C8 being the most retentive. A simple isocratic HPLC method that is based on simple protein precipitation was developed and validated for the determination of LVT in saliva. The method was applied to a sample group of epileptic children for the purpose of assessing potential correlation with plasma LVT levels and to investigate patient’s compliance. The results confirmed a reasonable correlation between plasma and salivary levels of LVT (R = 0.9) which supports the use of saliva for TDM of LVT. The study also revealed a significant percentage of epileptic patients having LVT levels below the estimated therapeutic range.

scholarly journals HPLC method development/validation and skin diffusion study of caffeine, methyl paraben and butyl paraben as skin–diffusing model drugs

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0247879
Author(s):  
Randa S. H. Mansour ◽  
Imad I. Hamdan ◽  
Mutaz S. H. Salem ◽  
Enam A. Khalil ◽  
ALSayed A. Sallam
Keyword(s):  
Method Development ◽  
Stationary Phases ◽  
Skin Permeation ◽  
Hplc Method ◽  
Gradient System ◽  
Diffusion Behavior ◽  
Methyl Paraben ◽  
Satisfactory Outcome ◽  
Peak Broadening ◽  
Wide Range

The focus of this research was to develop and validate a suitable HPLC method, which allows simultaneous determination of three proposed skin model penetrants to investigate the percutaneous diffusion behavior of their combination: caffeine, methyl paraben and butyl paraben. These penetrants were selected because they represent a wide range of lipophilicities. This model highlights the effect of combining penetrants of different molecular properties on their diffusion behavior through skin. The proposed method employed a gradient system that was systematically optimized for separation and quantification of the penetrants. The effect of the stationary phase (C18, C4 and cyano (CN)) was assessed with CN proven to be superior in terms of peak shape, retentivity and dynamic linear range. Significant differences in retention time, peak broadening, and quantifiability between different stationary phases could be demonstrated. The method was validated as per ICH guidelines Q2 (R1) with a satisfactory outcome. The method was successfully applied for real diffusion experiments, and revealed notable differences between the individual penetrants and their ternary mixture on transdermal permeation. The method could potentially be extended to determine these analytes in other related skin permeation investigations.

RP-HPLC method development for simultaneous determination of the drugs Ramipril and Amlodipine

2012 ◽  
Vol 2 (2) ◽  
pp. 364-367 ◽  
Author(s):  
Saida Naik Dheeravath ◽  
◽  
Kasani Ramadevi ◽  
Zilla Saraswathi ◽  
Dheeravath Maniklal ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Method Development ◽  
Hplc Method ◽  
Rp Hplc ◽  
Hplc Method Development
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