scholarly journals Coumarin-based fluorescent probe for the rapid detection of peroxynitrite ‘AND’ biological thiols

RSC Advances ◽  
2020 ◽  
Vol 10 (23) ◽  
pp. 13496-13499 ◽  
Author(s):  
Luling Wu ◽  
Xue Tian ◽  
Robin R. Groleau ◽  
Jie Wang ◽  
Hai-Hao Han ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Rapid Detection ◽  
Simultaneous Detection

A coumarin-based novel ‘AND’ logic fluorescent probe ROS-AHC has been developed for the simultaneous detection of ONOO− and biological thiols.

The recent advance of organic fluorescent probe rapid detection for common substances in beverages

2021 ◽  
pp. 129839
Author(s):  
Ning Duan ◽  
Shaoxiang Yang ◽  
Hongyu Tian ◽  
Baoguo Sun
Keyword(s):  
Fluorescent Probe ◽  
Rapid Detection

scholarly journals MnO2@Reduced Graphene Oxide Nanocomposite-Based Electrochemical Sensor for the Simultaneous Determination of Trace Cd(II), Zn(II) and Cu(II) in Water Samples

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 517
Author(s):  
Siyamthanda Hope Mnyipika ◽  
Tshimangadzo Saddam Munonde ◽  
Philiswa Nosizo Nomngongo
Keyword(s):  
Heavy Metals ◽  
Electrochemical Sensor ◽  
Simultaneous Determination ◽  
Rapid Detection ◽  
Electrochemical Sensors ◽  
Simultaneous Detection ◽  
Cyclic Voltammograms ◽  
Current Response ◽  
Mno2 Nanoparticles

The rapid detection of trace metals is one of the most important aspect in achieving environmental monitoring and protection. Electrochemical sensors remain a key solution for rapid detection of heavy metals in environmental water matrices. This paper reports the fabrication of an electrochemical sensor obtained by the simultaneous electrodeposition of MnO2 nanoparticles and RGO nanosheets on the surface of a glassy carbon electrode. The successful electrodeposition was confirmed by the enhanced current response on the cyclic voltammograms. The XRD, HR-SEM/EDX, TEM, FTIR, and BET characterization confirmed the successful synthesis of MnO2 nanoparticles, RGO nanosheets, and MnO2@RGO nanocomposite. The electrochemical studies results revealed that MnO2@RGO@GCE nanocomposite considerably improved the current response on the detection of Zn(II), Cd(II) and Cu(II) ions in surface water. These remarkable improvements were due to the interaction between MnO2 nanomaterials and RGO nanosheets. Moreover, the modified sensor electrode portrayed high sensitivity, reproducibility, and stability on the simultaneous determination of Zn(II), Cd(II), and Cu(II) ions. The detection limits of (S/N = 3) ranged from 0.002–0.015 μg L−1 for the simultaneous detection of Zn(II), Cd(II), and Cu(II) ions. The results show that MnO2@RGO nanocomposite can be successfully used for the early detection of heavy metals with higher sensitivity in water sample analysis.

FLGS-01. Development of novel topical fluorescent probe for intraoperative rapid detection of glioma

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi225-vi226
Author(s):  
Shota Tanaka ◽  
Yosuke Kitagawa ◽  
Mako Kamiya ◽  
Takenori Shimizu ◽  
Yasuteru Urano ◽  
...  
Keyword(s):  
Malignant Glioma ◽  
Fluorescent Probe ◽  
Rapid Detection ◽  
Aminolevulinic Acid ◽  
Characteristic Curve ◽  
Screening Method ◽  
Tumor Resection ◽  
Phase Iii ◽  
Glioma Surgery ◽  
Two Dimensional Gel Electrophoresis

Abstract PURPOSE Fluorescence imaging is an important surgical adjunct in malignant glioma surgery. 5-aminolevulinic acid (5-ALA) has been proven effective for radical tumor resection and extended progression-free survival in a phase III randomized trial and therefore integrated into surgery for malignant glioma. Importantly, however, some limitations still exist in its use, which include false positivity and false negativity as well as inability of re-administration. In this study, we aimed to develop a novel, spray-type fluorescent probe using hydroxymethyl rhodamine green (HMRG) as a fluorescent scaffold. METHODS We have previously established a fluorescent probe library comprised of more than 320 kinds of HMRG probes. They have HMRG as a fluorescent scaffold with various types of dipeptides attached to it. Primary probe screening was performed using the homogenized tumor samples from patients with glioblastoma operated at our institution. Secondary screening followed using the selected probes and fresh tumor samples obtained from patients with glioblastoma operated from 2016 until 2018. Diced electrophoresis gel (DEG) assay, two-dimensional gel electrophoresis followed by a multi-well plate-based fluorometric assay, was performed to identify responsible enzymes for the selected probe. Further experiments with inhibitors, real-time PCR, immunohistochemistry, and western blotting were performed for confirmation. RESULTS Proline-arginine-HMRG (PR-HMRG) was selected as a candidate probe based upon the above two-step screenings. It achieved 79.4% accuracy in receiver operating characteristic curve analysis. Calpain-1 was found to be responsible to cleave PR-HMRG probe by DEG-proteome analysis. Calpain-1 protein was highly expressed in tumor tissues which reacted to PR-HMRG probe. CONCLUSIONS Our innovative screening method was able to find PR-HMRG as a novel fluorescent probe effective for rapid detection of glioblastoma. A preclinical study is planned to assess the efficacy and safety of the selected probe.

A dual-emission and mitochondria-targeted fluorescent probe for rapid detection of SO2 derivatives and its imaging in living cells

Talanta ◽  
2019 ◽  
Vol 191 ◽  
pp. 428-434 ◽  
Author(s):  
Guoxing Yin ◽  
Yabing Gan ◽  
Ting Yu ◽  
Tingting Niu ◽  
Peng Yin ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Rapid Detection ◽  
Living Cells ◽  
Dual Emission

A fluorescent probe for rapid detection of estrogen receptors

Cancer ◽  
1980 ◽  
Vol 46 (S12) ◽  
pp. 2902-2906 ◽  
Author(s):  
B. Ramanath Rao ◽  
C. Glenn Fry ◽  
Steven Hunt ◽  
Radka Kuhnel ◽  
Walter B. Dandliker
Keyword(s):  
Estrogen Receptors ◽  
Fluorescent Probe ◽  
Rapid Detection

BODIPY-based fluorescent probe for the simultaneous detection of glutathione and cysteine/homocysteine at different excitation wavelengths

RSC Advances ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 3959-3964 ◽  
Author(s):  
Li-Ya Niu ◽  
Qing-Qing Yang ◽  
Hai-Rong Zheng ◽  
Yu-Zhe Chen ◽  
Li-Zhu Wu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Simultaneous Detection

We reported a BODIPY-based fluorescent probe for the simultaneous detection of GSH and Cys/Hcy.

A Duplex SYBR Green I-based Real-time Polymerase Chain Reaction Assay for Rapid Detection of Canine Kobuvirus and Canine Circovirus

2021 ◽  
Author(s):  
Yang Pan ◽  
Jing Chen ◽  
Junhuang Wu ◽  
Yongxia Wang ◽  
Junwei Zou ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Rapid Detection ◽  
Simultaneous Detection ◽  
Sybr Green ◽  
Sybr Green I ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain ◽  
Canine Kobuvirus

Abstract Background: Canine Kobuvirus (CaKoV) and Canine Circovirus (CaCV) are viruses that infect dogs causing diarrheal symptoms that are very similar. However, there is no clinical method to detect a co-infection of these two viruses.Results: In this study, a duplex SYBR Green I-based quantitative real-time polymerase chain reaction (PCR) assay for the rapid and simultaneous detection of CaKoV and CaCV was established. CaKoV and CaCV were distinguished by their different melting temperature which was 86℃ for CaKoV and 78℃ for CaCV. The assay was highly specific, with no cross-reactivity with other common canine viruses and demonstrated high sensitivity. The detection limits of CaKoV and CaCV were 8.924 × 101 copies/μL and 3.841 × 101 copies/μL, respectively. The highest intra- and inter-assay Ct value variation coefficients (CV) of CaKoV were 0.40% and 0.96%, respectively. For CaCV, the highest intra- and inter-assay Ct value variation coefficients were 0.26% and 0.70%, respectively. In 57 clinical samples, positive detection rates of CaKoV and CaCV were 8.77% (7/57) and 15.79% (9/57), respectively. The co-infection rate was 7.02% (4/57). Conclusions: The duplex SYBR Green I-based real-time PCR assay established in this study is a fast, efficient, and sensitive method for the simultaneous detection of the two viruses and provides a powerful tool for the rapid detection of CaKoV and CaCV in clinical practice.

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