scholarly journals The development of peptide–boron difluoride formazanate conjugates as fluorescence imaging agents

RSC Advances ◽  
2020 ◽  
Vol 10 (32) ◽  
pp. 18970-18977
Author(s):  
Neha Sharma ◽  
Stephanie M. Barbon ◽  
Tyler Lalonde ◽  
Ryan R. Maar ◽  
Mark Milne ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Cancer Imaging ◽  
Imaging Agents ◽  
Receptor Targeting ◽  
Boron Difluoride

Conjugation of a boron difluoride formazanate dye to receptor targeting peptides provides cancer imaging agents for fluorescence microscopy.

scholarly journals Low-Light Photodetectors for Fluorescence Microscopy

2021 ◽  
Vol 11 (6) ◽  
pp. 2773
Author(s):  
Hiroaki Yokota ◽  
Atsuhito Fukasawa ◽  
Minako Hirano ◽  
Toru Ide
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Single Molecule ◽  
Science Studies ◽  
Single Photon ◽  
Laser Scanning Microscopy ◽  
Oxide Semiconductor ◽  
Wide Field ◽  
Single Molecule Fluorescence ◽  
Low Light

Over the years, fluorescence microscopy has evolved and has become a necessary element of life science studies. Microscopy has elucidated biological processes in live cells and organisms, and also enabled tracking of biomolecules in real time. Development of highly sensitive photodetectors and light sources, in addition to the evolution of various illumination methods and fluorophores, has helped microscopy acquire single-molecule fluorescence sensitivity, enabling single-molecule fluorescence imaging and detection. Low-light photodetectors used in microscopy are classified into two categories: point photodetectors and wide-field photodetectors. Although point photodetectors, notably photomultiplier tubes (PMTs), have been commonly used in laser scanning microscopy (LSM) with a confocal illumination setup, wide-field photodetectors, such as electron-multiplying charge-coupled devices (EMCCDs) and scientific complementary metal-oxide-semiconductor (sCMOS) cameras have been used in fluorescence imaging. This review focuses on the former low-light point photodetectors and presents their fluorescence microscopy applications and recent progress. These photodetectors include conventional PMTs, single photon avalanche diodes (SPADs), hybrid photodetectors (HPDs), in addition to newly emerging photodetectors, such as silicon photomultipliers (SiPMs) (also known as multi-pixel photon counters (MPPCs)) and superconducting nanowire single photon detectors (SSPDs). In particular, this review shows distinctive features of HPD and application of HPD to wide-field single-molecule fluorescence detection.

Synthesis and evaluation of new salicylaldehyde-2-picolinylhydrazone Schiff base compounds of Ru(II), Rh(III) and Ir(III) as in vitro antitumor, antibacterial and fluorescence imaging agents

2015 ◽  
Vol 20 (4) ◽  
pp. 619-638 ◽  
Author(s):  
Narasinga Rao Palepu ◽  
S. L. Nongbri ◽  
J. Richard Premkumar ◽  
Akalesh Kumar Verma ◽  
Kaushik Bhattacharjee ◽  
...  
Keyword(s):  
Schiff Base ◽  
Fluorescence Imaging ◽  
Imaging Agents

Synthesis of Carbon-11 Labeled Triphenylacetamides as Novel Potential PET Melanoma Cancer Imaging Agents

Synthesis ◽  
2006 ◽  
Vol 2006 (14) ◽  
pp. 2301-2304
Author(s):  
Qi-Huang Zheng ◽  
Mingzhang Gao ◽  
Deyuan Kong ◽  
Abraham Clearfield ◽  
Kathy Miller ◽  
...  
Keyword(s):  
Cancer Imaging ◽  
Imaging Agents ◽  
Melanoma Cancer

scholarly journals Evaluation of an Integrin αvβ3 and Aminopeptidase N Dual-Receptor Targeting Tracer for Breast Cancer Imaging

2019 ◽  
Vol 17 (1) ◽  
pp. 349-358 ◽  
Author(s):  
Yongkang Gai ◽  
Yaqun Jiang ◽  
Yu Long ◽  
Lingyi Sun ◽  
Qingyao Liu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Imaging ◽  
Integrin Αvβ3 ◽  
Aminopeptidase N ◽  
Breast Cancer Imaging ◽  
Receptor Targeting

scholarly journals Fluorescence Imaging/Agents in Tumor Resection

2017 ◽  
Vol 28 (4) ◽  
pp. 569-583 ◽  
Author(s):  
Walter Stummer ◽  
Eric Suero Molina
Keyword(s):  
Fluorescence Imaging ◽  
Tumor Resection ◽  
Imaging Agents

Quantitative imaging of lymph function

2007 ◽  
Vol 292 (6) ◽  
pp. H3109-H3118 ◽  
Author(s):  
Ruchi Sharma ◽  
Wei Wang ◽  
John C. Rasmussen ◽  
Amit Joshi ◽  
Jessica P. Houston ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Quantitative Imaging ◽  
Imaging Agents ◽  
Excitation Light ◽  
Lymph Vessels ◽  
Intradermal Administration ◽  
Nir Fluorescence ◽  
Lymph Endothelium ◽  
Lymphatic Imaging

Functional lymphatic imaging was demonstrated in the abdomen and anterior hindlimb of anesthetized, intact Yorkshire swine by using near-infrared (NIR) fluorescence imaging following intradermal administration of 100–200 μl of 32 μM indocyanine green (ICG) and 64 μM hyaluronan NIR imaging conjugate to target the lymph vacular endothelial receptor (LYVE)-1 on the lymph endothelium. NIR fluorescence imaging employed illumination of 780 nm excitation light (∼2 mW/cm2) and collection of 830 nm fluorescence generated from the imaging agents. Our results show the ability to image the immediate trafficking of ICG from the plexus, through the vessels and lymphangions, and to the superficial mammary, subiliac, and middle iliac lymph nodes, which were located as deep as 3 cm beneath the tissue surface. “Packets” of ICG-transited lymph vessels of 2–16 cm length propelled at frequencies of 0.5–3.3 pulses/min and velocities of 0.23–0.75 cm/s. Lymph propulsion was independent of respiration rate. In the case of the hyaluronan imaging agent, lymph propulsion was absent as the dye progressed immediately through the plexus and stained the lymph vessels and nodes. Lymph imaging required 5.0 and 11.9 μg of ICG and hyaluronan conjugate, respectively. Our results suggest that microgram quantities of NIR optical imaging agents and their conjugates have a potential to image lymph function in patients suffering from lymph-related disorders.

scholarly journals Receptor-targeting fluorescence imaging and theranostics using a graphene oxide based supramolecular glycocomposite

2015 ◽  
Vol 3 (47) ◽  
pp. 9182-9185 ◽  
Author(s):  
Ding-Kun Ji ◽  
Yue Zhang ◽  
Yi Zang ◽  
Wang Liu ◽  
Xiongwen Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Imaging ◽  
Receptor Targeting

We describe the construction of a supramolecular, graphene oxide (GO)-based glycocomposite for receptor-targeting theranostics.

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