scholarly journals Interaction effects on a gold nanoparticle-based colorimetric assay for antioxidant capacity evaluation of polyphenols

RSC Advances ◽  
2020 ◽  
Vol 10 (25) ◽  
pp. 14705-14713
Author(s):  
Yanhui Wang ◽  
Shiqi Liu ◽  
Mengnan Yang ◽  
Ahmed Aboueloyoun Taha ◽  
Jing Wang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Gold Nanoparticle ◽  
Colorimetric Assay ◽  
Interaction Effects ◽  
Capacity Evaluation

Interaction effects of endogenous polyphenols remarkably influenced the performance of gold nanoparticle-based colorimetric assay for antioxidant capacity evaluation.

Gold nanoparticle-based colorimetric assay for cancer diagnosis

2010 ◽  
Vol 25 (8) ◽  
pp. 1869-1874 ◽  
Author(s):  
Jeong-Hun Kang ◽  
Yoji Asami ◽  
Masaharu Murata ◽  
Hirotaro Kitazaki ◽  
Noriaki Sadanaga ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Cancer Diagnosis ◽  
Colorimetric Assay

Total antioxidant capacity evaluation: Critical steps for assaying berry antioxidant features

BioFactors ◽  
2005 ◽  
Vol 23 (4) ◽  
pp. 221-227 ◽  
Author(s):  
Jessica Scalzo ◽  
Bruno Mezzetti ◽  
Maurizio Battino
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Capacity Evaluation ◽  
Total Antioxidant

Antioxidant Capacity Evaluation In Different Extravirgin Olive Oils

2010 ◽  
Vol 42 ◽  
pp. 793
Author(s):  
Giorgio Galanti ◽  
Matteo Becatti ◽  
Niccolò Taddei ◽  
Laura Stefani ◽  
Claudia Fiorillo
Keyword(s):  
Antioxidant Capacity ◽  
Olive Oils ◽  
Capacity Evaluation

PSXV-27 Late-Breaking: Evaluation of antioxidant capacity in receiving beef calves challenged with lipopolysaccharide (LPS)

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 381-382
Author(s):  
Samantha N Barker ◽  
Treylr Jackson ◽  
John Richeson ◽  
Jeff A Carroll ◽  
Nicole C Burdick-Sanchez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Repeated Measures ◽  
Colorimetric Assay ◽  
Post Challenge ◽  
Antioxidant Power ◽  
Blood Samples ◽  
Endotoxin Challenge ◽  
Beef Calves ◽  
Lps Challenge

Abstract The objective of this study was to evaluate antioxidant capacity in plasma of beef calves challenged with LPS. Following an initial feeding period of 40 d, steers (n = 32; 379 kg ± 30.7) were transported to the Livestock Issues Research Unit’s Bovine Immunology Research and Development Complex and challenged intravenously with LPS (0.25 µg/kg BW) on d 41. Blood samples were collected via jugular catheter at -2, 0, 2, 4, 6, 8, 10, 12, 18, 24, 36 and 48 h relative to the LPS challenge at 0 h. Blood samples were processed to isolate plasma for indicators of oxidative stress with a colorimetric assay to determine ferric reducing antioxidant power (FRAP) values via concentration of ferrous iron (µM). Data were analyzed as repeated measures using the GLIMMIX procedure of SAS. Antioxidant values did vary with time (P < 0.001) being greater (P < 0.05) at -2, 0, 2, 36, and 48 h. Antioxidant capacity was reduced at 6 and 8 h (P < 0.05), with the least FRAP value observed at 8 h post-challenge. Antioxidant capacity increased (P < 0.05) again at 10 h, showing similar (P > 0.05) concentrations to those observed at 4 h. By 24 h post-challenge, plasma FRAP values increased (P < 0.05) similar to initial values at -2, 0, and 2 h. It can be inferred that oxidative stress contributes to reduced antioxidant capacity, ultimately interfering with animal growth and productivity. While these values reflect the oxidative stress response to an acute endotoxin challenge, and a subsequent recovery returning to homeostasis within 24 to 48 h, they may also correlate with other physiological and immunological indicators associated with an acute endotoxin challenge.

Homogeneous, unmodified gold nanoparticle-based colorimetric assay of hydrogen peroxide

2007 ◽  
Vol 584 (1) ◽  
pp. 122-128 ◽  
Author(s):  
Zai-Sheng Wu ◽  
Song-Bai Zhang ◽  
Meng-Meng Guo ◽  
Chen-Rui Chen ◽  
Guo-Li Shen ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Gold Nanoparticle ◽  
Colorimetric Assay

Multiplex Detection of Viral DNAs in Blood by Colorimetrically Identifying Polymerase Chain Reaction Amplicons with Serial Invasive Reaction Assisted Gold Nanoparticle Probes Assembling

2020 ◽  
Vol 20 (10) ◽  
pp. 6140-6147
Author(s):  
Yi Ma ◽  
Chunyan Gong ◽  
Xiemin Qi ◽  
Bingjie Zou ◽  
Qinxin Song ◽  
...  
Keyword(s):  
Real Time ◽  
Gold Nanoparticle ◽  
Real Time Pcr ◽  
Pathogen Detection ◽  
Colorimetric Assay ◽  
Treponema Pallidum ◽  
Multiplex Detection ◽  
Clinical Practices ◽  
Resource Limited ◽  
Viral Dnas

Detection of blood-borne pathogenic viruses is essential for blood transfusion, and has great significance for epidemiology, as well as clinical practices. Common blood-borne viruses causing infectious diseases include Hepatitis B virus (HBV), Hepatitis C virus (HCV), Human immunodeficiency virus (HIV) and Treponema pallidum (TP). Therefore, multiplex detection of these viruses is more in the line with the needs of clinical testing. Although real-time PCR-based multiplex nucleic acid testing (NAT) was developed for pathogen detection, however, the requirement of multichannel realtime PCR machine increases the instrumental cost and is not suitable for use in resource-limited areas. In this study, we proposed a multiplex and colorimetric assay for detecting viral nucleic acids in blood by using serial invasive reaction assisted gold nanoparticle (AuNPs) probes assembling to identify multiplex PCR amplicons. As low as 2 copies per reaction of HIV and TP targets, and 20 copies per reaction of HBV and HCV targets can be detected. The results can be observed by naked eyes; thus, just a standard PCR machine is required. In addition, the hairpin probe and the AuNPs for signal read out are universal for all the targets, reducing the detection cost. About 20 DNA samples remaining after clinical HBV testing were successfully detected, and the results were consistent with that of commercially available real-time PCR based kit, indicating that this method has a potential for clinical applications.

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