scholarly journals The use of principle component analysis and MALDI-TOF MS for the differentiation of mineral forming Virgibacillus and Bacillus species isolated from sabkhas

RSC Advances ◽  
2020 ◽  
Vol 10 (25) ◽  
pp. 14606-14616 ◽  
Author(s):  
Rim Abdel Samad ◽  
Zulfa Al Disi ◽  
Mohammad Yousaf Mohammad Ashfaq ◽  
Sara Mohiddin Wahib ◽  
Nabil Zouari
Keyword(s):  
Principle Component Analysis ◽  
Component Analysis ◽  
Bacillus Species ◽  
Maldi Tof Ms ◽  
Principle Component ◽  
Maldi Tof ◽  
Tof Ms

Occurrence of mineral forming and other bacteria in mats is well demonstrated.

scholarly journals The use of Principle Component Analysis and MALDI-TOF MS for the differentiation of mineral forming Virgibacillus and Bacillus species isolated from Sabkhas

2020 ◽  
Author(s):  
Rim Abdel Samad ◽  
Zulfa Al Disi ◽  
Mohammad Ashfaq ◽  
Nabil Zouari
Keyword(s):  
Clustering Analysis ◽  
Potential Role ◽  
Protein Profiling ◽  
Bacillus Species ◽  
Pca Analysis ◽  
Bacterial Strains ◽  
Protein Biomarkers ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Occurrence of mineral forming and other bacteria in mats is well demonstrated. However, their high diversity shown by ribotyping was not explained, although it could explain the diversity of formed minerals. Common biomarkers as well as phylogenic relationships are useful tools to clustering the isolates and predict their potential role in the natural niche. In this study, combination of MALDI-TOF MS with PCA was shown a powerful tool to categorize 35 mineral forming bacterial strains isolated from Dohat Fshaikh sabkha, at northwest of Qatar (23 from decaying mats and 12 from living ones). 23 strains from decaying mats belong to Virgibacillus genus as identified by ribotyping and are shown highly involved in formation of protodolomite and a diversity of minerals. They were used as internal references in categorization of sabkha bacteria. Combination of isolation of bacteria on selective mineral forming media, their MALDI TOF MS protein profiling and PCA analysis established their relationship in a phyloproteomic based on protein biomarkers including m/z 4905, 3265, 5240, 6430, 7765, and 9815. PCA analysis clustered the studied strains into 3 major clusters, showing strong correspondence to the 3 phyloproteiomic groups that were established by the dendrogram. Both clustering analysis means have evidently demonstrated a relationship between known Virgibacillus strains and other related bacteria based on profiling of their synthesized proteins. Thus, larger populations of bacteria in mats can be easily screened for their potential to exhibit certain activities, which is of ecological, environmental and biotechnological significance.

Principal component analysis of MALDI-TOF MS of whole-cell foodborne pathogenic bacteria

2020 ◽  
Vol 592 ◽  
pp. 113582 ◽  
Author(s):  
Wenjing Yan ◽  
Jing Qian ◽  
Yongjie Ge ◽  
Keping Ye ◽  
Cunshan Zhou ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Pathogenic Bacteria ◽  
Principal Component ◽  
Component Analysis ◽  
Whole Cell ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Foodborne Pathogenic Bacteria ◽  
Tof Ms

Characterization of paraffin oils and petrolatum using LDI-TOF MS and principle component analysis

2003 ◽  
Vol 105 (6) ◽  
pp. 275-280 ◽  
Author(s):  
Julia K. Prunsa ◽  
Claudius Rapp ◽  
Ulrich Hintze ◽  
Klaus-Peter Wittern ◽  
Wilfried A. König
Keyword(s):  
Principle Component Analysis ◽  
Component Analysis ◽  
Principle Component ◽  
Tof Ms

Collagen-based proteinaceous binder-pigment interaction study under UV ageing conditions by MALDI-TOF-MS and principal component analysis

2012 ◽  
Vol 47 (3) ◽  
pp. 322-330 ◽  
Author(s):  
Julia Romero-Pastor ◽  
Natalia Navas ◽  
Stepanka Kuckova ◽  
Alejandro Rodríguez-Navarro ◽  
Carolina Cardell
Keyword(s):  
Principal Component Analysis ◽  
Principal Component ◽  
Component Analysis ◽  
Interaction Study ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Uv Ageing ◽  
Proteinaceous Binder ◽  
Tof Ms

scholarly journals Principal component analysis of MALDI TOF MS mass spectra separates M. abscessus (sensu stricto) from M. massiliense isolates

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Jan Kehrmann ◽  
Sarah Wessel ◽  
Roshni Murali ◽  
Annegret Hampel ◽  
Franz-Christoph Bange ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Mass Spectra ◽  
Principal Component ◽  
Component Analysis ◽  
Sensu Stricto ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

HPTLC-MALDI/TOF/MS vs. HPLC-ESI/MS2 for identification to flavonoids in plant extracts

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
L Fougère ◽  
D Da Silva ◽  
E Destandau ◽  
C Elfakir
Keyword(s):  
Plant Extracts ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Infektionserreger beim Schwein – Analyse des kultivierbaren Mikrobioms mittels MALDI-TOF MS

2017 ◽  
Author(s):  
M Erhard ◽  
M Metzner ◽  
D Köhler-Repp ◽  
B Köhler ◽  
R Storandt
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
Sign in / Sign up

Export Citation Format

Share Document