scholarly journals LC-MS/MS determination of avanafil and its metabolites in rat plasma and brain: pharmacokinetic study after oral administration and transdermal film application

RSC Advances ◽  
2020 ◽  
Vol 10 (16) ◽  
pp. 9407-9413
Author(s):  
Ahmed K. Kammoun ◽  
Alaa Khedr ◽  
Osama A. A. Ahmed
Keyword(s):  
Oral Administration ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Transdermal Application ◽  
Transdermal Film

A pioneering bioanalytical LC-MS/MS method was developed for monitoring avanafil and its metabolites in rat plasma and brain after oral administration and transdermal application.

scholarly journals Simultaneous Determination and Pharmacokinetic Comparisons of Multi-Ingredients after Oral Administration ofRadix Salviae MiltiorrhizaeExtract, Hawthorn Extract, and a Combination of Both Extracts to Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Yu-Qiang Liu ◽  
Qian Cai ◽  
Chang Liu ◽  
Feng-Wei Bao ◽  
Zhen-Qiu Zhang
Keyword(s):  
Oral Administration ◽  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Rosmarinic Acid ◽  
Rat Plasma ◽  
Hplc Method ◽  
Radix Salviae Miltiorrhizae ◽  
Salviae Miltiorrhizae ◽  
Hawthorn Extract

A simple and sensitive HPLC method was developed for simultaneous determination of danshensu (DSS), rosmarinic acid (RA), lithospermic acid (LA), salvianolic acid B (SAB), and hyperoside (HP) in rat plasma. This method validated was successfully applied to the pharmacokinetic study of the main active ingredients after oral administration ofRadix Salviae Miltiorrhizaeextract (SME), hawthorn extract (HTE), and a combination of both extracts (2.5 : 1) to rats. The results indicated that there have been great differences in pharmacokinetics between a single extract and a combination of both extracts. A combination of both extracts can enhance their bioavailabilities and delay the elimination of SAB and DSS in rats.

scholarly journals The Determination of 10 Compounds of GuiZhi Decoction in Rat Plasma after Oral Administration by HPLC-MS/MS and its Application to a Pharmacokinetic Study

2021 ◽  
Author(s):  
Huan Gao ◽  
Qin Guo ◽  
Lishi Zhang ◽  
Jiannan Song ◽  
Dong Bai ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Oral Administration ◽  
Lower Limit ◽  
Intravenous Injection ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Glycyrrhetinic Acid ◽  
Concentration Time ◽  
Guizhi Decoction

Abstract Background: Guizhi Decoction (GZD), a traditional Chinese medical formula, has been commonly used to treat fever, sweating, and cold in China. Methods: The high performance liquid chromatography -tandem mass spectrometry ( HPLC-MS/MS ) method was established for the determination of 10 compounds, including cinnamic acid , paeoniflorin, albiflorin, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, glycyrrhetinic acid, and 6-gingerol. And the specificity, linearity, lower limit of quantification (LLOQ), lower limit of detection (LLOD), precision and accuracy, recovery, matrix effect, and stability were used to verify the HPLC-MS/MS method. This validated method was successfully applied for pharmacokinetic study of the 10 compounds in rat plasma after oral administration of GZD in three doses (40 g crude drug·kg −1 , 20 g crude drug·kg −1 , 10 g crude drug·kg −1 ) and intravenous injection of GZD extraction at a dose of 2.0 g crude drug·kg −1 .The measurements of pharmacokinetic parameters including AUC 0–∞ , T 1/2 , T max , C max , Vz_F, Cl_F, and MRT, were performed using a non-compartmental model with Winnonlin 8.1 software. Results: The results showed that 10 compounds were detected in plasma after oral administration of GZD. the compounds (except for glycyrrhetinic acid) reached the maximum blood concentration quickly, whose Tmax was about 0.1-0.2 min. And a total of 9 compounds were detected after intravenous injection of GZD. The plasma concentration-time curve of these compounds declines rapidly at the beginning, and then decreased slowly, indicating that the plasma concentration-time curves were double exponential function curves. Conclusions: In this study, the developed method was suitable for pharmacokinetic analysis of the main compounds of GZD in rat plasma, and may reveal the pharmacodynamic material basis of GZD and provide a reference for the rational use of GZD in the clinic.

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