Determination of association constants and FRET in hydrazide-based molecular duplex strands

2020 ◽  
Vol 7 (17) ◽  
pp. 2419-2425
Author(s):  
Shi-Chang Gao ◽  
Kang Wan ◽  
Xu Fang ◽  
Yong-Xue Li ◽  
Min Xue ◽  
...  
Keyword(s):  
Association Constants ◽  
Substitution Reactions ◽  
Excimer Emission

The association constants for the hydrazide-based molecular duplex strands can be determined via monitoring the pyrene excimer emission. By mixing pyrene and perylene labelled oligomers, supramolecular substitution reactions induced efficient FRET.

Determination of association constants at moderately fast chemical exchange: Complexation of camphor enantiomers by α-cyclodextrin

2006 ◽  
Vol 181 (2) ◽  
pp. 304-309 ◽  
Author(s):  
Piotr Bernatowicz ◽  
Michał Nowakowski ◽  
Helena Dodziuk ◽  
Andrzej Ejchart
Keyword(s):  
Chemical Exchange ◽  
Association Constants ◽  
Fast Chemical

scholarly journals Interaction of rice (Oryza sativa) lectin with N-acetylglucosaminides. Fluorescence studies

1985 ◽  
Vol 229 (3) ◽  
pp. 687-692 ◽  
Author(s):  
F Tabary ◽  
J P Frénoy
Keyword(s):  
Oryza Sativa ◽  
Binding Sites ◽  
Wheat Germ ◽  
Equilibrium Dialysis ◽  
Blue Shift ◽  
Association Constants ◽  
Fluorescence Studies ◽  
Saccharide Binding ◽  
Binding Enthalpy

The interaction of lectin isolated from rice (Oryza sativa) embryos with N-acetylglucosaminides was studied by equilibrium dialysis and fluorescence. Equilibrium dialysis with 4-methylumbelliferyl-(GlcNac)2 showed that rice lectin (Mr 38000) contains four equivalent saccharide-binding sites. Addition of the N-acetylglucosaminides GlcNac, (GlcNac)2 and (GlcNac)3 enhanced the intrinsic fluorescence of rice lectin and this was accompanied by a 10nm blue-shift of its maximum fluorescence with (GlcNac)2 and (GlcNac)3. These changes in intensity allowed determination of the association constants, which increased with the number of saccharide units: at 20 degrees C, Ka = (1.3 +/- 0.1) X 10(3), (5.1 +/- 0.4) X 10(4) and (2.6 +/- 0.1) X 10(5) M−1 for GlcNac, (GlcNac)2 and (GlcNac)3 respectively. The binding enthalpy, delta H0, for the three glucosaminides were very low and ranged from −12.1 to −20.6 kJ X mol-1. The results are compared with those obtained with wheat-germ agglutinin, another GlcNac-specific gramineaous lectin.

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