Structural and Mechanical Characteristics of Exosomes from Osteosarcoma Cells Explored by 3D-Atomic Force Microscopy

Nanoscale ◽  
2021 ◽  
Author(s):  
Ayhan Yurtsever ◽  
Takeshi Yoshida ◽  
Arash Badami Behjat ◽  
Yoshinori Araki ◽  
Rikinari Hanayama ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Atomic Force Microscopy ◽  
Drug Delivery Systems ◽  
Mechanical Characteristics ◽  
Cell Communication ◽  
Delivery Systems ◽  
Osteosarcoma Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Potential Biomarkers

Exosomes have recently gained interest as mediators of cell-to-cell communication and as potential biomarkers for cancer and other diseases. They also have potential as nanocarriers for drug delivery systems. Therefore,...

scholarly journals Polymeric Nanostructures for Drug Delivery: Characterization by Atomic Force Microscopy

2005 ◽  
Vol 11 (S03) ◽  
pp. 36-39 ◽  
Author(s):  
V. C. F. Mosqueira ◽  
E. A. Leite ◽  
C. M. Barros ◽  
J. M. C. Vilela ◽  
M. S. Andrade
Keyword(s):  
Drug Delivery ◽  
Atomic Force Microscopy ◽  
One Dimension ◽  
Free Base ◽  
Lipophilic Drugs ◽  
Force Microscopy ◽  
Atomic Force ◽  
Essential Components ◽  
Formal Definitions ◽  
Lipophilic Character

Formal definitions of nanotechnological devices for drug delivery typically feature the requirements that the device itself or its essential components be man-made, and in the 1-1000 nm range in at least one dimension [1]. The known nanovectors or nanostructures can be filled with drugs for different therapies and for diagnostical aims. Targeting moieties can also be attached to their surface. Polymeric nanovectors are generally made from biodegradable polymers such as polyesters, for example, poly-e-caprolactone (PCL). The drug delivery system known as nanocapsules (NCs) can be defined as a complex nanovector that is composed by a polymeric wall surrounding an oil core, where lipophilic drugs can be encapsulated. The advantages of NCs compared to other nanovectors are the high entrapment efficiencies of lipophilic drugs, low polymer content and low inherent toxicity. On the other hand, because of its complex blend of components NCs suspension allow several forms of nanovectors to be present at the same time, such as nanospheres, liposomes and nanoemulsions [2]. These ‘contaminants’ would be present in accordance with the type of formulation and method of preparation. Atomic force microscopy (AFM) has been used as a method for imaging the surfaces of liposomes [3] and nanospheres [4] allowing information in nanoscaled dimensions. In the present work, the NCs were prepared loading two different drugs, the antifungal albaconazole (ABZ), showing a crystalline drug structure and the antimalarial halofantrine (Hf) free base, having an amorphous form. These drugs possess high lipophilic character, which favours the association of the drug with the oily core, with drug loadings above 94%. Herein we studied the behavior of ABZ-loaded and Hf-loaded NCs through the AFM technique, searching to analyze and understand possible alterations induced by the drug inclusion in these nanostructures.

Probing Mechanical Characteristics of Living Fibroblasts via Atomic Force Microscopy

2019 ◽  
Vol 45 (9) ◽  
pp. 947-950 ◽  
Author(s):  
K. I. Timoshchuk ◽  
M. M. Khalisov ◽  
V. A. Penniyaynen ◽  
B. V. Krylov ◽  
A. V. Ankudinov
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Characteristics ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals О молекулярной природе различий в реакции сенсорных нейронов и фибробластов на уабаин

2021 ◽  
Vol 91 (5) ◽  
pp. 882
Author(s):  
М.М. Халисов ◽  
В.А. Пеннияйнен ◽  
С.А. Подзорова ◽  
А.В. Анкудинов ◽  
К.И. Тимощук ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Sensory Neurons ◽  
Intracellular Signaling ◽  
Mechanical Characteristics ◽  
Signaling Cascades ◽  
Force Microscopy ◽  
Atomic Force ◽  
Endogenous Ouabain ◽  
Membrane Rigidity ◽  
Pumping Function

Atomic force microscopy was used to study under physiologically adequate conditions the effect of ouabain on the mechanical characteristics of sensory neurons and fibroblasts of 10–12-day old chick embryos. Fibroblasts express only the α1-isoform of Na,K-ATPase, and sensory neurons the α1- and α3-isoforms. It was found that the action of ouabain at a concentration corresponding to the endogenous value leads to an increase in the membrane rigidity of sensory neurons, which is apparently due to the activation of the transducer function of Na,K-ATPase, rather than the pumping function. The endogenous concentration of ouabain did not change the mechanical characteristics of fibroblasts. The results obtained suggest that endogenous ouabain modulates the transducer function of the α3-isoform of Na,K-ATPase of the sensory neuron membrane. Thus, the method of atomic force microscopy allows a comparative study of intracellular signaling cascades in living cells.

Steel Pile-Up Characterization by Atomic Force Microscopy

2017 ◽  
Vol 891 ◽  
pp. 78-82 ◽  
Author(s):  
Peter Burik ◽  
Pavol Zubko ◽  
Ladislav Pešek ◽  
Lukáš Voleský
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Young’S Modulus ◽  
Mechanical Characteristics ◽  
The Real ◽  
Force Microscopy ◽  
Contact Depth ◽  
Steel Sheets ◽  
Atomic Force ◽  
Pile Up

The Oliver–Pharr method has extensively been adopted for measuring hardness and Young’s modulus by indentation techniques. However, the method assumes that the contact periphery sinks in, which limits the applicability to the materials pile-up [1]. In this work, we characterize the pile-up (shape and height) in steel sheets with different mechanical properties and propose an improved methodology to calculate the real mechanical characteristics of steel sheets with significant pile-up. Pile-up correction of mechanical characteristics is based on ratio of pile-up height and contact depth. Pile-up height was measured by atomic force microscopy (AFM).

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