scholarly journals Tuning DNA–nanoparticle conjugate properties allows modulation of nuclease activity

Nanoscale ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 4956-4970
Author(s):  
Jeff C. Hsiao ◽  
Tomas Buryska ◽  
Eunjung Kim ◽  
Philip D. Howes ◽  
Andrew J. deMello
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acids ◽  
Systematic Study ◽  
Nuclease Activity ◽  
Modular Units ◽  
Spherical Nucleic Acids

A systematic study of the interactions between nucleases and oligonucleotide-coated gold nanoparticles (spherical nucleic acids, SNAs) demonstrates that the modular units of SNAs can be leveraged to either accelerate or suppress nuclease kinetics.

scholarly journals Spherical Nucleic Acids for Topical Treatment of Hyperpigmentation

2021 ◽  
Vol 143 (3) ◽  
pp. 1296-1300
Author(s):  
Yang Fang ◽  
Xueguang Lu ◽  
Dali Wang ◽  
Jiansong Cai ◽  
Yuyan Wang ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Topical Treatment ◽  
Spherical Nucleic Acids

Synergistic Immunostimulation through the Dual Activation of Toll-like Receptor 3/9 with Spherical Nucleic Acids

ACS Nano ◽  
2021 ◽  
Author(s):  
Ziyin N. Huang ◽  
Cassandra E. Callmann ◽  
Lisa E. Cole ◽  
Shuya Wang ◽  
Chad A. Mirkin
Keyword(s):  
Nucleic Acids ◽  
Toll Like Receptor ◽  
Dual Activation ◽  
Spherical Nucleic Acids

scholarly journals Controlled Monofunctionalization of Molecular Spherical Nucleic Acids on a Buckminster Fullerene Core

2021 ◽  
Author(s):  
Vijay Gulumkar ◽  
Antti Äärelä ◽  
Olli Moisio ◽  
Jani Rahkila ◽  
Ville Tähtinen ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Buckminster Fullerene ◽  
Spherical Nucleic Acids

scholarly journals Gene Regulation Using Spherical Nucleic Acids to Treat Skin Disorders

2020 ◽  
Vol 13 (11) ◽  
pp. 360
Author(s):  
Thomas R. Holmes ◽  
Amy S. Paller
Keyword(s):  
Nucleic Acids ◽  
Mouse Models ◽  
Phase 1 ◽  
Diabetic Mouse ◽  
Intratumoral Injection ◽  
Skin Disorders ◽  
Ganglioside Gm3 ◽  
Human Trials ◽  
Accelerate Wound Healing ◽  
Spherical Nucleic Acids

Spherical nucleic acids (SNAs) are nanostructures consisting of nucleic acids in a spherical configuration, often around a nanoparticle core. SNAs are advantageous as gene-regulating agents compared to conventional gene therapy owing to their low toxicity, enhanced stability, uptake by virtually any cell, and ability to penetrate the epidermal barrier. In this review we: (i) describe the production, structure and properties of SNAs; (ii) detail the mechanism of SNA uptake in keratinocytes, regulated by scavenger receptors; and (iii) report how SNAs have been topically applied and intralesionally injected for skin disorders. Specialized SNAs called nanoflares can be topically applied for gene-based diagnosis (scar vs. normal tissue). Topical SNAs directed against TNFα and interleukin-17A receptor reversed psoriasis-like disease in mouse models and have been tested in Phase 1 human trials. Furthermore, SNAs targeting ganglioside GM3 synthase accelerate wound healing in diabetic mouse models. Most recently, SNAs targeting toll-like receptor 9 are being used in Phase 2 human trials via intratumoral injection to induce immune responses in Merkel cell and cutaneous squamous cell carcinoma. Overall, SNAs are a valuable tool in bench-top and clinical research, and their advantageous properties, including penetration into the epidermis after topical delivery, provide new opportunities for targeted therapies.

Spherical nucleic acids start rolling

Science ◽  
2015 ◽  
Vol 349 (6253) ◽  
pp. 1150-1151 ◽  
Author(s):  
Robert F. Service
Keyword(s):  
Nucleic Acids ◽  
Spherical Nucleic Acids

Poly-adenine-mediated spherical nucleic acids for strand displacement-based DNA/RNA detection

2019 ◽  
Vol 127 ◽  
pp. 85-91 ◽  
Author(s):  
Lu Wang ◽  
Huan Zhang ◽  
Chenguang Wang ◽  
Yi Xu ◽  
Jing Su ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Strand Displacement ◽  
Rna Detection ◽  
Displacement Based ◽  
Spherical Nucleic Acids

scholarly journals The Spherical Nucleic Acids mRNA Detection Paradox

2016 ◽  
Author(s):  
Marie Held ◽  
Raphael Levy
Keyword(s):  
Gold Nanoparticles ◽  
Mrna Level ◽  
Membrane Vesicles ◽  
Apparent Paradox ◽  
Intracellular Vesicle ◽  
Intracellular Sensing ◽  
The 1950S ◽  
Spherical Nucleic Acids ◽  
New Applications ◽  
Endocytic Compartments

<p>From the 1950s onwards, our understanding of the formation and intracellular trafficking of membrane vesicles was informed by experiments in which cells were exposed to gold nanoparticles and their uptake and localisation, studied by electron microscopy.&nbsp; In the last decade, building on progress in the synthesis of gold nanoparticles and their controlled functionalisation with a large variety of biomolecules (DNA, peptides, polysaccharides), new applications have been proposed, including the imaging and sensing of intracellular events. Yet, as already demonstrated in the 1950s, uptake of nanoparticles results in confinement within an intracellular vesicle which in principle should preclude sensing of cytosolic events. To study this apparent paradox, we focus on a commercially available nanoparticle probe that detects mRNA through the release of a fluorescently-labelled oligonucleotide (unquenching the fluorescence) in the presence of the target mRNA. Using electron, fluorescence and photothermal microscopy, we show that the probes remain in endocytic compartments and that they do not report on mRNA level. We suggest that the validation of any nanoparticle-based probes for intracellular sensing should include a quantitative and thorough demonstration that the probes can reach the cytosolic compartment.</p>

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