scholarly journals Isolation of DNA aptamers targeting N-cadherin and high-efficiency capture of circulating tumor cells by using dual aptamers

Nanoscale ◽  
2020 ◽  
Vol 12 (44) ◽  
pp. 22574-22585
Author(s):  
Tian Gao ◽  
Pi Ding ◽  
Wenjing Li ◽  
Zhili Wang ◽  
Qiao Lin ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Efficiency ◽  
Dna Aptamers

Isolation of anti-N-cadherin DNA aptamers and high-efficiency capture of circulating tumor cells using dual aptamers targeting EpCAM and N-cadherin.

Peptide-based isolation of circulating tumor cells by magnetic nanoparticles

2014 ◽  
Vol 2 (26) ◽  
pp. 4080-4088 ◽  
Author(s):  
Linling Bai ◽  
Yimeng Du ◽  
Jiaxi Peng ◽  
Yi Liu ◽  
Yanmei Wang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Efficiency ◽  
Isolation Method ◽  
Functionalized Magnetic Nanoparticles

A new CTC isolation method with high efficiency by using EpCAM recognition peptide functionalized magnetic nanoparticles was developed.

scholarly journals High efficiency vortex trapping of circulating tumor cells

2015 ◽  
Vol 9 (6) ◽  
pp. 064116 ◽  
Author(s):  
Manjima Dhar ◽  
Jessica Wong ◽  
Armin Karimi ◽  
James Che ◽  
Corinne Renier ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Efficiency

High-Efficiency Light-Induced Circulating Tumor Cells Screening Biochip Prepared by 40.68-MHz VHFPECVD

2020 ◽  
Vol 20 (16) ◽  
pp. 8938-8946
Author(s):  
Jia-Hao Lin ◽  
Hung-Wei Wu ◽  
Wei-Chen Tien ◽  
Cheng-Yuan Hung ◽  
Hong-Yue Lai ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Efficiency

Isolation of Circulating Tumor Cells Using Stiffness-Based Filtration Platform

2015 ◽  
Author(s):  
Emrah Celik ◽  
Nicolas Rongione ◽  
Amelia Bahamonde ◽  
Zheng Ao ◽  
Ram Datar
Keyword(s):  
Tumor Cell ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Blood Cells ◽  
High Efficiency ◽  
White Blood Cells ◽  
Cell Isolation ◽  
Element Analysis ◽  
Cancer Disease

Analysis of isolated cancer cells in circulation is proven to help determine the success of the cancer treatment and understand the genetic signature of cancer disease. Scarcity of these cells in blood circulation (1–10 CTC in 1ml blood) however, makes the isolation process extremely challenging. Ever improving CTC isolation methods fall into two main categories: 1.Immunomagnetic separation based on antibody binding to tumor specific biomarkers expressed on the cell 2. Physical separation based on the size of the CTCs. Efficiency in cell isolation is still low in these techniques due to the variation in expression level of tumor specific antigens and tumor cell size. Therefore, tumor cell isolation strategies using new CTC biomarkers must be explored. In this study, we investigated the feasibility of using mechanical stiffness difference in order to detect and isolate the circulating tumor cells from the blood cells. AFM nanindentation experiments revealed that cancer cells are significantly softer than the surrounding white blood cells and therefore, stiffness can be used as a biomarker for CTC isolation. In addition, finite element analysis simulations have shown that CTC isolation can be performed at high efficiency using stiffness-based isolation. Therefore, stiffness based isolation has a potential to achieve fast, label-free isolation of CTCs at high efficiency for clinical applications.

scholarly journals Design and Clinical Application of an Integrated Microfluidic Device for Circulating Tumor Cells Isolation and Single-Cell Analysis

Micromachines ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 49
Author(s):  
Mingxin Xu ◽  
Wenwen Liu ◽  
Kun Zou ◽  
Song Wei ◽  
Xinri Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Efficiency ◽  
Single Cell Analysis ◽  
Cell Damage ◽  
Capture Rate ◽  
Advanced Lung Cancer ◽  
Cell Analysis

Circulating tumor cells (CTCs) have been considered as an alternative to tissue biopsy for providing both germline-specific and tumor-derived genetic variations. Single-cell analysis of CTCs enables in-depth investigation of tumor heterogeneity and individualized clinical assessment. However, common CTC enrichment techniques generally have limitations of low throughput and cell damage. Herein, based on micropore-arrayed filtration membrane and microfluidic chip, we established an integrated CTC isolation platform with high-throughput, high-efficiency, and less cell damage. We observed a capture rate of around 85% and a purity of 60.4% by spiking tumor cells (PC-9) into healthy blood samples. Detection of CTCs from lung cancer patients demonstrated a positive detectable rate of 87.5%. Additionally, single CTCs, ctDNA and liver biopsy tissue of a representative advanced lung cancer patient were collected and sequenced, which revealed comprehensive genetic information of CTCs while reflected the differences in genetic profiles between different biological samples. This work provides a promising tool for CTCs isolation and further analysis at single-cell resolution with potential clinical value.

scholarly journals Molecular profiling of single circulating tumor cells from lung cancer patients

2016 ◽  
Vol 113 (52) ◽  
pp. E8379-E8386 ◽  
Author(s):  
Seung-min Park ◽  
Dawson J. Wong ◽  
Chin Chun Ooi ◽  
David M. Kurtz ◽  
Ophir Vermesh ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Efficiency ◽  
Tumor Biology ◽  
Metastatic Tumor ◽  
Lung Cancer Patients ◽  
Single Ctcs ◽  
Mutation Profiling

Circulating tumor cells (CTCs) are established cancer biomarkers for the “liquid biopsy” of tumors. Molecular analysis of single CTCs, which recapitulate primary and metastatic tumor biology, remains challenging because current platforms have limited throughput, are expensive, and are not easily translatable to the clinic. Here, we report a massively parallel, multigene-profiling nanoplatform to compartmentalize and analyze hundreds of single CTCs. After high-efficiency magnetic collection of CTC from blood, a single-cell nanowell array performs CTC mutation profiling using modular gene panels. Using this approach, we demonstrated multigene expression profiling of individual CTCs from non–small-cell lung cancer (NSCLC) patients with remarkable sensitivity. Thus, we report a high-throughput, multiplexed strategy for single-cell mutation profiling of individual lung cancer CTCs toward minimally invasive cancer therapy prediction and disease monitoring.

scholarly journals Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood

Cancers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 351 ◽  
Author(s):  
Galina Zamay ◽  
Olga Kolovskaya ◽  
Tatiana Ivanchenko ◽  
Tatiana Zamay ◽  
Dmitry Veprintsev ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells ◽  
Clinical Samples ◽  
Dna Aptamers ◽  
Microscopy Imaging ◽  
Lung Cancer Patients

We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients.

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