Salient features of medical nanoparticles in biological fluids from an analytical ultracentrifuge

Nanoscale ◽  
2020 ◽  
Vol 12 (44) ◽  
pp. 22462-22466
Author(s):  
Gizem Cinar ◽  
Christoph Englert ◽  
Ulrich S. Schubert ◽  
Ivo Nischang
Keyword(s):  
Biological Fluids ◽  
Analytical Ultracentrifuge ◽  
Stepping Stone ◽  
Salient Features

Deciphering the behavior of degradable nanoparticles in biological fluids is demonstrated by interference-free assessment. The resultant vastly different erosion characteristics provide a necessary stepping stone for their design toward translation.

Purification of Urokinase from Complex Mixtures Using Immobilized Monoclonal Antibody Against Urokinase Light Chain

1983 ◽  
Vol 49 (01) ◽  
pp. 024-027 ◽  
Author(s):  
David Vetterlein ◽  
Gary J Calton
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Culture Media ◽  
Biological Fluids ◽  
Single Step ◽  
High Yield ◽  
Step Method ◽  
Commercial Preparation ◽  
E Coli ◽  
Tissue Culture Media

SummaryThe preparation of a monoclonal antibody (MAB) against high molecular weight (HMW) urokinase light chain (20,000 Mr) is described. This MAB was immobilized and the resulting immunosorbent was used to isolate urokinase starting with an impure commercial preparation, fresh urine, spent tissue culture media, or E. coli broth without preliminary dialysis or concentration steps. Monospecific antibodies appear to provide a rapid single step method of purifying urokinase, in high yield, from a variety of biological fluids.

The Measurement of Heparin and Other Therapeutic Sufphated Polysaccharides in Plasma, Serum and Urine

1985 ◽  
Vol 54 (03) ◽  
pp. 630-634 ◽  
Author(s):  
J Dawes ◽  
C V Prowse ◽  
D D Pepper
Keyword(s):  
Biological Fluids ◽  
Anticoagulant Activity ◽  
Dose Range ◽  
Plasma Concentrations ◽  
Heparin Therapy ◽  
Competitive Binding Assay ◽  
First Order Kinetics ◽  
Activity Measurements ◽  
Heparin Activity ◽  
Dose Dependent

SummaryThe competitive binding assay described will specifically and accurately measure concentrations of administered heparin in biological fluids with a sensitivity of 60 ng ml-1. Neither endogenous glycosaminoglycans, nor plasma proteins such as ATIII and PF4 interfere in the assay. Semi-synthetic highly sulphated heparinoids and LMW heparin can also be measured. Using this assay heparin clearance followed simple first-order kinetics over the dose range 100-5,000 units, but the half-life was strongly dose-dependent. There was good correlation with heparin activity measurements by APTT and anti-Xa clotting assays. Plasma concentrations were measurable for at least 5 h following subcutaneous injection of 10,000 units of heparin. Excretion in the urine could be followed after all but the lowest intravenous dose. This assay, used in conjunction with measurements of heparin anticoagulant activity, will be valuable in the elucidation of mechanisms of action of heparin and the heparinoids, and in the assessment and management of problems related to heparin therapy.

The Japanese Factor in the Making of North Korean Socialism

2020 ◽  
pp. 225-251
Author(s):  
Ernest Ming-Tak Leung
Keyword(s):  
Communist Party ◽  
North Korea ◽  
Colonial Rule ◽  
Direct Experience ◽  
Colonial Era ◽  
Stepping Stone ◽  
The Creation ◽  
Juche Ideology

This article explores a commonly ignored aspect of Japan–North Korean relations: the Japanese factor in the making of Korean socialism. Korea was indirectly influenced by the Japanese Jiyuminken Movement, in the 1910s–1920s serving as a stepping-stone for the creation of a Japanese Communist Party. Wartime mobilization policies under Japanese rule were continued and expanded beyond the colonial era. The Juche ideology built on tendencies first exhibited in the 1942 Overcoming Modernity Conference in Japan, and in the 1970s some Japanese leftists viewed Juche as a humanist Marxism. Trade between Japan and North Korea expanded from 1961 onwards, culminating in North Korea’s default in 1976, from which point on relations soured between the two countries. Yet leaders with direct experience of colonial rule governed North Korea through to the late 1990s.

Inclusive Fitness in Finite Deme-structured and Stepping-stone Populations

Selection ◽  
2001 ◽  
Vol 1 (1-3) ◽  
pp. 153-164 ◽  
Author(s):  
P. D. Taylor ◽  
A. J. Irwin ◽  
T. Day
Keyword(s):  
Inclusive Fitness ◽  
Stepping Stone

Metastable Defects in the Amorphous Silicon-Germanium Alloys

2003 ◽  
Vol 762 ◽  
Author(s):  
J. David Cohen
Keyword(s):  
Amorphous Silicon ◽  
Silicon Germanium ◽  
Dangling Bonds ◽  
Annealing Behavior ◽  
Fundamental Properties ◽  
Silicon Materials ◽  
Salient Features ◽  
The Individual ◽  
Amorphous Silicon Germanium ◽  
Silicon Germanium Alloys

AbstractThis paper first briefly reviews a few of the early studies that established some of the salient features of light-induced degradation in a-Si,Ge:H. In particular, I discuss the fact that both Si and Ge metastable dangling bonds are involved. I then review some of the recent studies carried out by members of my laboratory concerning the details of degradation in the low Ge fraction alloys utilizing the modulated photocurrent method to monitor the individual changes in the Si and Ge deep defects. By relating the metastable creation and annealing behavior of these two types of defects, new insights into the fundamental properties of metastable defects have been obtained for amorphous silicon materials in general. I will conclude with a brief discussion of the microscopic mechanisms that may be responsible.

Role of exosomes in diagnosis and therapy of prostate cancer

2020 ◽  
Vol 28 (3) ◽  
pp. 399-405
Author(s):  
Fabrizio Fontana ◽  
Olga A. Babenko
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Biological Fluids ◽  
Diagnosis And Treatment ◽  
Diagnosis And Therapy ◽  
The Future ◽  
Important Direction ◽  
Potential Biomarkers

Aim of this letter is to attract the attention of journal readers to the study of exosomes as an important direction in the development of Oncology, in particular, in the diagnosis and treatment of prostate cancer. Exosomes are produced by tumor cells and regulate proliferation, metastasis, and the development of chemoresistance. Their extraction from biological fluids allows further use of these vesicles as potential biomarkers of prostate cancer. In the future, exosomes can be successfully used in the delivery of drugs and other anti-tumor substances to cancer cells.

Sign in / Sign up

Export Citation Format

Share Document