scholarly journals Nanoscale flow cytometry for immunophenotyping and quantitating extracellular vesicles in blood plasma

Nanoscale ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 2012-2025 ◽  
Author(s):  
Nikki Salmond ◽  
Karan Khanna ◽  
Gethin R. Owen ◽  
Karla C. Williams
Keyword(s):  
Flow Cytometry ◽  
Blood Plasma ◽  
Extracellular Vesicles ◽  
Small Particles ◽  
In Blood Plasma

Nanoscale flow cytometry permits analysis of small particles in unpurified plasma and supports identification and quantitation of different particle populations.

scholarly journals Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering

Cells ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 1046 ◽  
Author(s):  
Božič ◽  
Sitar ◽  
Junkar ◽  
Štukelj ◽  
Pajnič ◽  
...  
Keyword(s):  
Light Scattering ◽  
Blood Plasma ◽  
Extracellular Vesicles ◽  
Viscosity Coefficient ◽  
Plausible Model ◽  
Average Shape ◽  
Key Factor ◽  
Spherical Vesicles ◽  
In Blood Plasma

Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be ρ ~ 1 (0.94–1.1 in exosome standards and 0.7–1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity.

scholarly journals Formation of a protein corona on the surface of extracellular vesicles in blood plasma

2021 ◽  
Vol 10 (11) ◽  
Author(s):  
Eszter Á. Tóth ◽  
Lilla Turiák ◽  
Tamás Visnovitz ◽  
Csaba Cserép ◽  
Anett Mázló ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Extracellular Vesicles ◽  
Protein Corona ◽  
In Blood Plasma

State of hormonal regulation in experimental animals exposed to noise and vibration

2020 ◽  
pp. 680-681
Author(s):  
A. V. Lizarev ◽  
V. A. Pankov
Keyword(s):  
Blood Plasma ◽  
Hormonal Regulation ◽  
Adrenocorticotropic Hormone ◽  
Vibration Exposure ◽  
Noise And Vibration ◽  
Experimental Animals ◽  
In Blood Plasma

When exposed to noise and vibration in experimental animals there was a decrease in the content of threeiodinethyronine, thyroxin and adrenocorticotropic hormone in blood plasma after 15 and 30 days of experience. An increase in loads led to an increase in the level of threeiodinethyronine and thyroxin under vibration exposure and was normalized with noise. The content of adrenocorticotropic hormone leveled in both cases.

EFFECT OF DIETARY LEVELS OF SODIUM AND POTASSIUM ON GROWTH AND ON CONCENTRATIONS IN BLOOD PLASMA AND TISSUES OF WHITE RAT

1950 ◽  
Vol 162 (1) ◽  
pp. 182-188 ◽  
Author(s):  
J. H. Meyer ◽  
R. R. Grunert ◽  
Marie T. Zepplin ◽  
R. H. Grummer ◽  
G. Bohstedt ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Sodium And Potassium ◽  
In Blood Plasma

Two SPRi biosensors for the determination of cathepsin S in blood plasma

Talanta ◽  
2021 ◽  
Vol 225 ◽  
pp. 121900
Author(s):  
Lukasz Oldak ◽  
Anna Sankiewicz ◽  
Beata Żelazowska-Rutkowska ◽  
Bogdan Cylwik ◽  
Zenon Lukaszewski ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Cathepsin S ◽  
In Blood Plasma

scholarly journals DETERMINATION OF TRYPSIN INHIBITOR IN BLOOD PLASMA

1953 ◽  
Vol 204 (1) ◽  
pp. 147-152 ◽  
Author(s):  
Shirley F. McCann ◽  
M. Laskowski
Keyword(s):  
Blood Plasma ◽  
Trypsin Inhibitor ◽  
In Blood Plasma

scholarly journals The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows

2021 ◽  
Author(s):  
Jacek Wawrzykowski ◽  
Monika Jamioł ◽  
Wioleta Mojsym ◽  
Marta Kankofer
Keyword(s):  
Blood Plasma ◽  
Antioxidative Enzymes ◽  
Placental Development ◽  
Blood Samples ◽  
Bovine Placenta ◽  
Total Antioxidant ◽  
Tissue Homogenates ◽  
Further Development ◽  
General Profile ◽  
In Blood Plasma

AbstractPhysiological balance between pro- and antioxidative processes is crucial for placentation and further development of fetus and placenta. Parameters of pro- and antioxidative profile may serve as markers of proper course of pregnancy. The aim of study was to assess whether the balance between pro- and antioxidative parameters during placentation phase in bovine placenta is maintained. Placental and blood samples were collected from healthy, HF, pregnant (2nd-3rd month) cows (n = 8) in slaughterhouse and in farm, respectively. Formylokinurenine and bityrosine content were measured spectrofluorimetrically in blood plasma and tissue homogenates while metabolites of lipid peroxidation, total antioxidant capacity, SH groups and activity of antioxidative enzymes (glutathione peroxidase and superoxide dismutase) were determined in examined tissues by spectrophotometry. Western blotting was used to confirm the presence of enzymatic proteins in placenta. Results: Local profile in tissues was more pronounced than general profile in blood plasma. Activities of antioxidative enzymes were significantly (p < 0.05) higher in 2nd compared to 3rd month of pregnancy in maternal part of placenta while prooxidant parameters showed opposite relationship. Obtained results showed significant differences when compared to data from non-pregnant animals or time of parturition. Further studies are necessary for elucidation of placentation phase in cows.

Sign in / Sign up

Export Citation Format

Share Document