scholarly journals Single molecule distribution of RhD binding epitopes on ultraflat erythrocyte ghosts

Nanoscale ◽  
2020 ◽  
Vol 12 (43) ◽  
pp. 22097-22106
Author(s):  
Sarah Stainer ◽  
Sara Reisetbauer ◽  
Joan E. A. Ahiable ◽  
Leon Ebner ◽  
Rong Zhu ◽  
...  
Keyword(s):  
Blood Group ◽  
Single Molecule ◽  
Organ Transplant ◽  
Erythrocyte Ghost ◽  
Blood Group System ◽  
Erythrocyte Ghosts ◽  
Group System ◽  
Transplant Medicine ◽  
Recognition Imaging ◽  
Rh Blood Group

The Rh blood group system plays a key role in transfusion and organ transplant medicine. AFM-based recognition imaging on ulraflat erythrocyte ghost makes it possible to determine the molecular distribution of Rh epitopes over the entire erythrocyte.

scholarly journals The Rh blood group system: the first 60 years of discovery

2020 ◽  
Vol 16 (1) ◽  
pp. 7-17
Author(s):  
Christine Lomas-Francis ◽  
Marion E. Reid
Keyword(s):  
Blood Group ◽  
Blood Group System ◽  
Group System ◽  
Rh Blood Group

scholarly journals Rapid Rh D genotyping by polymerase chain reaction-based amplification of DNA

Blood ◽  
1995 ◽  
Vol 85 (10) ◽  
pp. 2975-2980 ◽  
Author(s):  
S Simsek ◽  
BH Faas ◽  
PM Bleeker ◽  
MA Overbeeke ◽  
HT Cuijpers ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Group ◽  
False Positive ◽  
Complete Agreement ◽  
Blood Group System ◽  
Chain Reaction ◽  
Group System ◽  
Allele Specific ◽  
Polymerase Chain ◽  
Rh Blood Group

Rh (rhesus) D is the dominant antigen of the Rh blood group system. Recent advances in characterization of the nucleotide sequence of the cDNA(s) encoding the Rh D polypeptide allow the determination of the Rh D genotype at the DNA level. This can be of help in cases in which red blood cells are not available for phenotyping, eg, when in concerns a fetus. We have tested three independent DNA typing methods based on the polymerase chain reaction (PCR) for their suitability to determine the Rh D genotype. DNA derived from peripheral blood mononuclear cells from 234 Rh-phenotyped healthy donors (178 Rh D positive and 56 Rh D negative) was used in the PCR. The Rh D genotypes, as determined with a method based on the allele-specific amplification of the 3′ noncoding region of the Rh D gene described by Bennett et al (N Engl J Med 329:607, 1993), were not concordant with the serologically established phenotypes in all cases. We have encountered 5 discrepant results, ie, 3 false-positive and 2 false-negative (a father and child). Rh D genotyping with the second method was performed by PCR amplification of exon 7 of the D gene with allele-specific primers. In all donors phenotyped as D positive tested so far (n = 178), the results of molecular genotyping with this method were concordant with the serologic results, whereas a false-positive result was obtained in one of the D-negative donors (also false-positive in the first method). Complete agreement was found between genotypes determined in the third method, based on a 600-bp deletion in intron 4 of the Rh D gene described by Arce et al (Blood 82:651, 1993), and serologically determined phenotypes. The Rh blood group system is complex, and unknown polymorphisms at the DNA level are expected to exist. Therefore, although genotypes determined by the method of Arce et al were in agreement with serotypes, it cannot yet be regarded as the golden standard. More experience with this or other methods is still needed.

Rh Blood Group System

2012 ◽  
pp. 147-262 ◽  
Author(s):  
Marion E. Reid ◽  
Christine Lomas-Francis ◽  
Martin L. Olsson
Keyword(s):  
Blood Group ◽  
Blood Group System ◽  
Group System ◽  
Rh Blood Group

ABO and Rh Blood Group System and Periodontal Disease - A Prevalence Study

2016 ◽  
Vol 16 (5) ◽  
pp. 1-6
Author(s):  
Patil Anup ◽  
Varma Siddhartha ◽  
Suragimath Girish ◽  
Abbayya Keshava ◽  
Zope Sameer ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Blood Group ◽  
Prevalence Study ◽  
Blood Group System ◽  
Group System ◽  
Rh Blood Group
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