Active-modulated, random-illumination, super-resolution optical fluctuation imaging

Nanoscale ◽  
2020 ◽  
Vol 12 (32) ◽  
pp. 16864-16874
Author(s):  
Baoju Wang ◽  
Zhijia Liu ◽  
Li Zhou ◽  
Yiyan Fei ◽  
Chengliang Yang ◽  
...  
Keyword(s):  
Super Resolution ◽  
Stochastic Intensity ◽  
Intensity Fluctuations ◽  
Random Illumination

Super-resolution optical fluctuation imaging (SOFI) provides subdiffraction resolution based on the analysis of temporal stochastic intensity fluctuations.

scholarly journals Super-resolved live-cell imaging using Random Illumination Microscopy

2020 ◽  
Author(s):  
Thomas Mangeat ◽  
Simon Labouesse ◽  
Marc Allain ◽  
Emmanuel Martin ◽  
Renaud Poincloux ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
New Technology ◽  
Super Resolution ◽  
Ease Of Use ◽  
Live Cell ◽  
Three Dimensions ◽  
Tissue Imaging ◽  
Wide Range ◽  
Random Illumination

SummarySuper-resolution fluorescence microscopy has been instrumental to progress in biology. Yet, the photo-induced toxicity, the loss of resolution into scattering samples or the complexity of the experimental setups curtail its general use for functional cell imaging. Here, we describe a new technology for tissue imaging reaching a 114nm/8Hz resolution at 30 µm depth. Random Illumination Microscopy (RIM) consists in shining the sample with uncontrolled speckles and extracting a high-fidelity super-resolved image from the variance of the data using a reconstruction scheme accounting for the spatial correlation of the illuminations. Super-resolution unaffected by optical aberrations, undetectable phototoxicity, fast image acquisition rate and ease of use, altogether, make RIM ideally suited for functional live cell imaging in situ. RIM ability to image molecular and cellular processes in three dimensions and at high resolution is demonstrated in a wide range of biological situations such as the motion of Myosin II minifilaments in Drosophila.

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 42-51
Author(s):  
S. S. Ryabichko ◽  
◽  
A. N. Ibragimov ◽  
L. A. Lebedeva ◽  
E. N. Kozlov ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Super Resolution ◽  
Structure And Function ◽  
And Function ◽  
Super Resolution Microscopy

Facial Image Super-Resolution Reconstruction Based on Separated Frequency Components

2013 ◽  
Vol E96.A (6) ◽  
pp. 1315-1322 ◽  
Author(s):  
Hyunduk KIM ◽  
Sang-Heon LEE ◽  
Myoung-Kyu SOHN ◽  
Dong-Ju KIM ◽  
Byungmin KIM
Keyword(s):  
Super Resolution ◽  
Facial Image ◽  
Frequency Components ◽  
Image Super Resolution
Sign in / Sign up

Export Citation Format

Share Document