In vivo photothermal inhibition of methicillin-resistant Staphylococcus aureus infection by in situ templated formulation of pathogen-targeting phototheranostics

Nanoscale ◽  
2020 ◽  
Vol 12 (14) ◽  
pp. 7651-7659 ◽  
Author(s):  
Xujuan Guo ◽  
Bing Cao ◽  
Congyu Wang ◽  
Siyu Lu ◽  
Xianglong Hu
Keyword(s):  
Staphylococcus Aureus ◽  
Magnetic Resonance ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Photoacoustic Imaging ◽  
Methicillin Resistant ◽  
Aureus Infection ◽  
Mrsa Infection ◽  
Efficient Elimination

Herein, pathogen-targeting phototheranostic nanoparticles, Van-OA@PPy, are in situ developed for efficient elimination of MRSA infection, which is reflected by dual-modality magnetic resonance and photoacoustic imaging.

scholarly journals Methicillin-Resistant Staphylococcus Aureus Infection Following Arthroscopy of the Knee Joint

2006 ◽  
Vol 88 (7) ◽  
pp. 675-676 ◽  
Author(s):  
D Raj ◽  
S Iyer ◽  
CM Fergusson
Keyword(s):  
Staphylococcus Aureus ◽  
Knee Joint ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Arthroscopic Surgery ◽  
Safe Procedure ◽  
Methicillin Resistant ◽  
Aureus Infection ◽  
Mrsa Infection

Arthroscopic surgery of the knee is considered to be a safe procedure. We had a microbiologically confirmed infection of methicillin-resistant Staphylococcus aureus (MRSA). Although various rare infective cases are reported following arthroscopy of the knee joint, to the best of our knowledge there is no previous report of MRSA infection following arthroscopy of the knee joint.

scholarly journals Antibacterial Properties of MagnesiumIn Vitroand in anIn VivoModel of Implant-Associated Methicillin-Resistant Staphylococcus aureus Infection

2014 ◽  
Vol 58 (12) ◽  
pp. 7586-7591 ◽  
Author(s):  
Yang Li ◽  
Guangwang Liu ◽  
Zanjing Zhai ◽  
Lina Liu ◽  
Haowei Li ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bone Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Periprosthetic Infection ◽  
Antibacterial Properties ◽  
Methicillin Resistant ◽  
Content Type ◽  
Aureus Infection

ABSTRACTPeriprosthetic infection remains a challenging clinical complication. We investigated the antibacterial properties of pure (99.9%) magnesium (Mg)in vitroand in anin vivorat model of implant-related infection. Mg was highly effective against methicillin-resistantStaphylococcus aureus-induced osteomyelitis and improved new peri-implant bone formation. BacterialicaAandagrRNAIII transcription levels were also assessed to characterize the mechanism underlying the antibacterial properties of the Mg implant.

scholarly journals Epigallocatechin Gallate Synergistically Enhances the Activity of Carbapenems against Methicillin-Resistant Staphylococcus aureus

2002 ◽  
Vol 46 (2) ◽  
pp. 558-560 ◽  
Author(s):  
Zhi-Qing Hu ◽  
Wei-Hua Zhao ◽  
Nozomi Asano ◽  
Yoshiyuki Yoda ◽  
Yukihiko Hara ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Epigallocatechin Gallate ◽  
Clinical Isolates ◽  
Main Constituent ◽  
Tea Catechins ◽  
Methicillin Resistant ◽  
Mrsa Infection

ABSTRACT Combinations of carbapenems and epigallocatechin gallate (EGCg; a main constituent of tea catechins) showed potent synergy against 24 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). MICs of imipenem in the presence of EGCg at 3.125, 6.25, 12.5, and 25 μg/ml, were restored to the susceptible breakpoint (≤4 μg/ml) for 8, 38, 46, and 75% of the MRSA isolates, respectively. Similar results were also observed for combinations of panipenem or meropenem and EGCg. Therefore, the combinations may be worthy of further evaluation in vivo against MRSA infection.

scholarly journals 1218. Retapamulin as a Potential Decolonizing Agent: Activity against Mupirocin-Resistant Strains From Pediatric Patients With Methicillin-Resistant Staphylococcus aureus Infection

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S369-S370
Author(s):  
Ami Patel ◽  
Jennifer Lighter-Fisher ◽  
Yi Fulmer ◽  
Richard Copin ◽  
Adam Ratner ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pediatric Patients ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Promising Alternative ◽  
Methicillin Resistant ◽  
Mrsa Infection ◽  
Mupirocin Resistance ◽  
Research Grant

Abstract Background Controlling methicillin-resistant Staphylococcus aureus (MRSA) colonization is a common strategy to prevent transmission and recurrent infection. Standard decolonization regimens include nasal application of mupirocin ointment; however, increasing rates of mupirocin-resistance (Mup-R) have been noted globally. At our institution there has been an increase in community-acquired MRSA (CA-MRSA) infections among children living in Brooklyn, New York. A genotypic geographic cluster of an outbreak clone of the CA-MRSA strain USA 300 with a high rate (>85%) of mupirocin resistance, mediated by the plasmid borne mupA gene, was identified prompting investigation into an alternative decolonizing agent. We sought to investigate retapamulin, a topical pleuromutilin antibiotic, which has been shown to be effective against S. aureus with in vitro and in vivo activity against MRSA and a low propensity to develop resistance. Methods Broth microdilution was used to determine the minimum inhibitory concentrations (MIC) of retapamulin against 53 Mup-R MRSA isolates collected from pediatric patients (aged 9 months–17 years) presenting to our institution over an 18 month period with clinical MRSA infection. Susceptibility defined as ≤0.5 mg/L susceptible (EUCAST). Whole genome sequence data were analyzed for the presence of rplC and cfr gene mutations known to confer resistance to retapamulin. Results All 53 isolates were susceptible to retapamulin. 49/53 (92%) strains were inhibited at MIC 0.25 mg/L, 2/53 (4%) at MIC 0.125 mg/L, and 2/53 (4%) at MIC 0.5 mg/L. DNA sequence analysis showed that one isolate had a first-step mutation in the rplC gene, but it was not associated with reduced phenotypic susceptibility to retapamulin, as the MIC of that isolate was 0.25 mg/L. Conclusion Retapamulin demonstrated excellent in vitro activity against a genotypic cluster of Mup-R isolates from pediatric patients presenting to our institution with MRSA infection. These data suggest that retapamulin may be a promising alternative decolonization therapy for MRSA and a viable option to prevent the spread of mupirocin-resistant MRSA clones. Further research includes an ongoing randomized, placebo-controlled trial testing the in vivo efficacy of retapamulin as a nasal and perirectal decolonizing agent in children. Disclosures A. Patel, Aqua Pharmaceuticals: Investigator inititiated grant, Research grant. J. Lighter-Fisher, Aqua Pharmaceuticals: Investigator Initiated Grant, Research grant.

scholarly journals In Vitro and In Vivo Activity of 14-O-[(4,6-Diamino-pyrimidine-2-yl) thioacetyl] Mutilin against Methicillin-Resistant Staphylococcus aureus

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3277
Author(s):  
Yunxing Fu ◽  
Chunqing Leng ◽  
Yuan Fan ◽  
Xia Ma ◽  
Xianghui Li ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Agent ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Skin Wound ◽  
Methicillin Resistant ◽  
Wound Model ◽  
New Antibiotics ◽  
Mrsa Infection

Staphylococcus aureus (S. aureus) is a major human pathogen that requires new antibiotics with unique mechanism. A new pleuromutilin derivative, 14-O-[(4,6-Diamino-pyrimidine-2-yl) thioacetyl] mutilin (DPTM), has been synthesized and proved as a potent antibacterial agent using in vitro and in vivo assays. In the present study, DPTM was further in vitro evaluated against methicillin-resistant Staphylococcus aureus (MRSA) isolated from dairy farms and outperformed tiamulin fumarate, a pleuromutilin drug used for veterinary. Moreover, a murine skin wound model caused by MRSA infection was established, and the healing effect of DPTM was investigated. The results showed that DPTM could promote the healing of MRSA skin infection, reduce the bacterial burden of infected skin MRSA and decrease the secretion of IL-6 and TNF-α inflammatory cytokines in plasma. These results provided the basis for further in-depth drug targeted studies of DPTM as a novel antibacterial agent.

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