Enantioseparation and determination of orphenadrine in rat plasma and its application to a stereoselective pharmacokinetic study

2021 ◽  
Vol 45 (12) ◽  
pp. 5428-5436
Author(s):  
Yanru Liu ◽  
Yushan Ding ◽  
Yongbo Song ◽  
Xingjie Guo
Keyword(s):  
Chiral Separation ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Pharmacokinetics Study ◽  
Stereoselective Pharmacokinetics

Chiral separation and stereoselective pharmacokinetics study of orphenadrine enantiomers in rats by HPLC-MS/MS.

LC–MS/MS Determination of Apigenin in Rat Plasma and Application to Pharmacokinetic Study

2020 ◽  
Vol 21 ◽  
Author(s):  
Shixing Zhu ◽  
Jiayuan Zhang ◽  
Zhihua Lv ◽  
Mingming Yu
Keyword(s):  
Formic Acid ◽  
Pharmacokinetic Study ◽  
Ammonium Acetate ◽  
Rat Plasma ◽  
Biological Properties ◽  
Plasma Samples ◽  
Supply Rate ◽  
Natural Plant ◽  
Ammonium Acetate Buffer

Background: Apigenin, a natural plant flavone, has been shown to possess a variety of biological properties. Objective: In this report, a highly selective and sensitive LC-MS/MS method was developed and validated for the determination of apigenin in rat plasma. Methods: Analysts were separated on the HSS T3 column (1.8 μm 2.1×100 mm) using acetonitrile and 0.1% formic acid in 2 mM ammonium acetate buffer at a supply rate of 0.200 mL/min as eluent in gradient model. Results: Plasma samples were treated by protein precipitation using acetonitrile for the recovery ranging from 86.5% to 90.1% for apigenin. The calibration curves followed linearity in the concentration range of 0.50-500 ng/mL. The inter-day and intra-day precisions at different QC levels within 13.1% and the accuracies ranged from -10.6% to 8.6%. Conclusion: The assay has been successfully applied to the pharmacokinetic study of apigenin in rats.

Determination of nootkatone in rat plasma by LC‐MS/MS and its application in a pharmacokinetic study

2021 ◽  
Author(s):  
Zhen‐miao Qin ◽  
Yong‐hui Li ◽  
Yin‐feng Tan ◽  
Hai‐long Li
Keyword(s):  
Pharmacokinetic Study ◽  
Rat Plasma
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