A novel bromelain-MnO2 biosensor for colorimetric determination of dopamine

2021 ◽  
Vol 45 (1) ◽  
pp. 92-97
Author(s):  
Shimeng Liu ◽  
Xiayu Zhou ◽  
Changyin Lv ◽  
Ran Liu ◽  
Shiya Li ◽  
...  
Keyword(s):  
Oxidase Activity ◽  
New Method ◽  
Colorimetric Determination ◽  
Colorimetric Sensing

We report a method for determining dopamine in serum via colorimetric sensing. This new method adopts bromelain-templated MnO2 nanosheets that possess high oxidase activity. 3,3′,5,5′-Tetramethylbenzidine (TMB) can be directly oxidized by bromelain-MnO2 nanosheets to oxTMB products.

A dynamic gas extraction-assisted paper-based method for colorimetric determination of bromides

2020 ◽  
Vol 12 (4) ◽  
pp. 587-594 ◽  
Author(s):  
Marina O. Gorbunova ◽  
Maria S. Garshina ◽  
Margarita S. Kulyaginova ◽  
Vladimir V. Apyari ◽  
Aleksei A. Furletov ◽  
...  
Keyword(s):  
Natural Waters ◽  
New Method ◽  
Colorimetric Determination ◽  
Gas Extraction ◽  
Selective Determination

A new method for selective determination of bromides in natural waters, foodstuffs and pharmaceuticals has been developed.

Measurement of Ceruloplasmin from Its Oxidase Activity in Serum by Use of o-Dianisidine Dihydrochloride

1974 ◽  
Vol 20 (12) ◽  
pp. 1556-1563 ◽  
Author(s):  
Karl H Schosinsky ◽  
H Peter Lehmann ◽  
Myrton F Beeler
Keyword(s):  
Coefficient Of Variation ◽  
Oxidase Activity ◽  
New Method ◽  
Radial Immunodiffusion ◽  
Normal Range ◽  
Serum Ceruloplasmin ◽  
Stable Product ◽  
Method R

Abstract We have developed a new method for determination of serum ceruloplasmin from its oxidase activity, in which o-dianisidine dihydrochloride is used as substrate. o-Dianisidine dihydrochloride is more stable than is the more widely used p-phenylenediamine substrate, and forms a stable product that is measured at 540 nm. Correlation was good between results of this method and those obtained with both a p-phenylenediamine substrate method (r = 0.98) and a radial immunodiffusion procedure (r = 0.97). There is little or no interference from reducing or colored components of serum. The coefficient of variation (day-to-day) for the method was 4.2%. A normal range of 62-140 U/ liter has been determined by the reported method.

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