A novel fluorescent assay for uracil DNA glycosylase activity built on the 3′–5′ exonuclease activity-based endonuclease IV cyclic signal amplification strategy

2020 ◽  
Vol 44 (48) ◽  
pp. 21211-21217
Author(s):  
Jing-Xuan Tian ◽  
Yan-Zhao Fang ◽  
Yi-Xuan Yang ◽  
Shuang Wu ◽  
Qiang Xiao ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Glycosylase ◽  
Exonuclease Activity ◽  
Sensitive Assay ◽  
Fluorescent Assay ◽  
Uracil Dna Glycosylase ◽  
Highly Sensitive ◽  
Dna Strands ◽  
Amplification Strategy

The unique 3′-5′ exonuclease activity of endonuclease IV to DNA strands has been demonstrated, which enables the development of a novel highly sensitive assay for UDG activity.

A highly sensitive assay for matrix metalloproteinase 2 via signal amplification strategy of eATRP

2021 ◽  
Vol 164 ◽  
pp. 106015
Author(s):  
Qingyu Wang ◽  
Jingliang Liu ◽  
Shuaibing Yu ◽  
Haobo Sun ◽  
Lei Wang ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Signal Amplification ◽  
Matrix Metalloproteinase 2 ◽  
Sensitive Assay ◽  
Highly Sensitive ◽  
Amplification Strategy

A label free fluorescent assay for uracil-DNA glycosylase activity based on the signal amplification of exonuclease I

RSC Advances ◽  
2015 ◽  
Vol 5 (98) ◽  
pp. 80871-80874 ◽  
Author(s):  
Yefei Ma ◽  
Jingjin Zhao ◽  
Xuejun Li ◽  
Liangliang Zhang ◽  
Shulin Zhao
Keyword(s):  
Signal Amplification ◽  
Dna Glycosylase ◽  
Label Free ◽  
Fluorescent Assay ◽  
Uracil Dna Glycosylase ◽  
Exonuclease I

A label free fluorescent assay for uracil-DNA glycosylase activity was developed based on the signal amplification of exonuclease I.

A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite

The Analyst ◽  
2021 ◽  
Vol 146 (8) ◽  
pp. 2679-2688
Author(s):  
Chammari Pothipor ◽  
Noppadol Aroonyadet ◽  
Suwussa Bamrungsap ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Highly Sensitive ◽  
Highly Dispersed ◽  
Polypyrrole Composite ◽  
Amplification Strategy ◽  
Dispersed Gold ◽  
Potential Tool

An ultrasensitive electrochemical biosensor based on a gold nanoparticles/graphene/polypyrrole composite modified electrode and a signal amplification strategy employing methylene blue is developed as a potential tool for the detection of miRNA-21.

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