Chromogenic and fluorogenic “off–on–off” chemosensor for selective and sensitive detection of aluminum (Al3+) and bifluoride (HF2−) ions in solution and in living Hep G2 cells: synthesis, experimental and theoretical studies

2020 ◽  
Vol 44 (31) ◽  
pp. 13259-13265 ◽  
Author(s):  
Samit Pramanik ◽  
Saikat Kumar Manna ◽  
Sudipta Pathak ◽  
Debasish Mondal ◽  
Kunal Pal ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Sensitive Detection ◽  
Theoretical Studies ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Ion Sensing ◽  
G2 Cells ◽  
Experimental And Theoretical Studies

A simple pyridine-dicarbohydrazide based colorimetric and fluorometric chemosensor L was designed and synthesized for Al3+ ion sensing in organo aqueous solution.

Glutamic Acid Uptake Inhibition Assay in Cultured Hep G2 Cells as an Alternative Method for Evaluating Potential In Vivo Eye Irritation

1989 ◽  
Vol 16 (4) ◽  
pp. 344-352
Author(s):  
Paul J. Dierickx
Keyword(s):  
Glutamic Acid ◽  
Corneal Opacity ◽  
Acid Uptake ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Eye Irritation ◽  
Uptake Inhibition ◽  
Range Finding ◽  
G2 Cells

Glutamic acid (GA) content was measured in cultured Hep G2 cells, after treatment of the cells with test compounds. The results with 37 chemicals were compared with their respective rabbit eye irritation data, of which 17 were determined according to the OECD test, and the other 20 in range-finding studies. The chemicals were mainly organic solvents (alcohols, esters, amines, acids and others). The xenobiotics were applied to the cells for 4 hours at 5 different concentrations. The cells were then incubated for 15 minutes with tritiated GA. GA uptake inhibition was measured by liquid scintillation counting, and the results were expressed as the GI50 value, which is the concentration of test compound required to induce a 50% reduction in GA uptake. A linear correlation coefficient r = 0.66 was found between the log GI50 and the mean corneal opacity scores. This value is comparable to that obtained in total protein and uridine uptake inhibition studies. However, r = 0.81 was found when the log GI50 was compared with range-finding scores, indicating that a closer relationship exists between cytotoxicity and the latter.

scholarly journals Human proapolipoprotein A-II is cleaved following secretion from Hep G2 cells by a thiol protease.

1984 ◽  
Vol 259 (24) ◽  
pp. 15556-15563 ◽  
Author(s):  
J I Gordon ◽  
H F Sims ◽  
C Edelstein ◽  
A M Scanu ◽  
A W Strauss
Keyword(s):  
Hep G2 ◽  
Hep G2 Cells ◽  
Thiol Protease ◽  
G2 Cells

The Hep G2 Cell Line as a Possible Alternative to Isolated Hepatocytes in Cytotoxicity Studies

1988 ◽  
Vol 16 (1) ◽  
pp. 16-22
Author(s):  
Marina Marinovich ◽  
Jose L. Lorenzo ◽  
Liliana M. Flaminio ◽  
Agnese Granata ◽  
Corrado L. Galli
Keyword(s):  
Cell Line ◽  
Rat Hepatocytes ◽  
Prostaglandin E ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Isolated Rat Hepatocytes ◽  
G2 Cells ◽  
Isolated Rat

The hepatotoxicity of carbon tetrachloride (CC14) was evaluated in vitro in freshly isolated rat hepatocytes and in the human hepatoma cell line, Hep G2. Toxicity was assessed by the leakage of cytosolic enzymes (lactate dehydrogenase and aspartate aminotransferase) and cell viability (trypan blue exclusion). The established human cells were less sensitive to CCl4-induced injury; higher doses of the toxic agent and longer incubation times were necessary to elicit cell damage. Micromolar concentrations of prostaglandin E2 significantly decreased enzyme leakage in both Hep G2 cells and rat hepatocytes challenged with CC14; a stable derivative of prostacyclin (ZK 36374) was ineffective. These results suggest that human hepatoma Hep G2 cells may represent a valid alternative to isolated rat hepatocytes for an initial approach to the in vitro evaluation of cell toxicity.

scholarly journals Studies of the sites of intracellular degradation of apolipoprotein B in Hep G2 cells.

1992 ◽  
Vol 267 (31) ◽  
pp. 22630-22638 ◽  
Author(s):  
S Furukawa ◽  
N Sakata ◽  
H.N. Ginsberg ◽  
J.L. Dixon
Keyword(s):  
Apolipoprotein B ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Intracellular Degradation ◽  
G2 Cells

Ankaflavin fromMonascus-Fermented Red Rice Exhibits Selective Cytotoxic Effect and Induces Cell Death on Hep G2 Cells

2005 ◽  
Vol 53 (6) ◽  
pp. 1949-1954 ◽  
Author(s):  
Nan-Wei Su ◽  
Yii-Lih Lin ◽  
Min-Hsiung Lee ◽  
Chen-Ying Ho
Keyword(s):  
Cell Death ◽  
Cytotoxic Effect ◽  
Red Rice ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells

Plasmodium falciparum : binding studies of peptide derived from the sporozoite surface protein 2 to Hep G2 cells

2001 ◽  
Vol 58 (4) ◽  
pp. 285-292 ◽  
Author(s):  
R. López ◽  
H. Curtidor ◽  
M. Urquiza ◽  
J. Garcia ◽  
A. Puentes ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Surface Protein ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Binding Studies ◽  
G2 Cells

Hepatoprotective constituents of the edible brown algaEcklonia stolonifera on tacrine-induced cytotoxicity in hep G2 cells

2005 ◽  
Vol 28 (12) ◽  
pp. 1376-1380 ◽  
Author(s):  
Youn Chul Kim ◽  
Ren Bo An ◽  
Na Young Yoon ◽  
Taek Jeong Nam ◽  
Jae Sue Choi
Keyword(s):  
Hep G2 ◽  
Hep G2 Cells ◽  
G2 Cells
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