Red emissive carbon dots obtained from direct calcination of 1,2,4-triaminobenzene for dual-mode pH sensing in living cells

2020 ◽  
Vol 44 (17) ◽  
pp. 7210-7217
Author(s):  
Yueyue Chen ◽  
Chan Wang ◽  
Yalan Xu ◽  
Guoxia Ran ◽  
Qijun Song
Keyword(s):  
Carbon Dots ◽  
Living Cells ◽  
Ph Sensing ◽  
Dual Mode ◽  
Ph Fluctuations

Red emissive carbon dots with colorimetric/fluorescent responses to pH fluctuations were prepared by direct calcination of 1,2,4-triaminobenzene.

Carbon dots doped by nitrogen and sulfur for dual-mode colorimetric and fluorometric determination of Fe3+ and histidine and intracellular imaging of Fe3+ in living cells

2020 ◽  
Vol 187 (10) ◽  
Author(s):  
Mojtaba Amiri ◽  
Ali Mohammad Haji Shabani ◽  
Shayessteh Dadfarnia ◽  
Nader Shokoufi ◽  
Behnam Hajipour-Verdom ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Living Cells ◽  
Dual Mode ◽  
Fluorometric Determination ◽  
Intracellular Imaging

scholarly journals Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 604 ◽  
Author(s):  
Maojia Huang ◽  
Xinyue Liang ◽  
Zixiao Zhang ◽  
Jing Wang ◽  
Yiyan Fei ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Fluorescence Lifetime ◽  
Fluorescence Intensity ◽  
Carbon Dots ◽  
Living Cells ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Gradual Change ◽  
Ph Sensing ◽  
Lifetime Imaging

The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study. In this research, pH-sensitive carbon dots (CDs) were employed for intracellular pH sensing with fluorescence lifetime imaging microscopy (FLIM) for the first time. FLIM is a highly sensitive method that is used to detect a microenvironment and it can overcome the limitations of biosensing methods based on fluorescence intensity. The different groups on the CDs surfaces changing with pH environments led to different fluorescence lifetime values. The CDs aqueous solution had a gradual change from 1.6 ns to 3.7 ns in the fluorescence lifetime with a pH range of 2.6–8.6. Similar fluorescence lifetime changes were found in pH buffer-treated living cells. The detection of lysosomes, cytoplasm, and nuclei in living cells was achieved by measuring the fluorescence lifetime of CDs. In particular, a phasor FLIM analysis was used to improve the pH imaging. Moreover, the effects of the coenzymes, amino acids, and proteins on the fluorescence lifetime of CDs were examined in order to mimic the complex microenvironment inside the cells.

Ratiometric pH Sensing in Living Cells Using Carbon Dots

2019 ◽  
Vol 37 (1) ◽  
pp. 1900430 ◽  
Author(s):  
Jun‐Ray Macairan ◽  
Issan Zhang ◽  
Adryanne Clermont‐Paquette ◽  
Rafik Naccache ◽  
Dusica Maysinger
Keyword(s):  
Carbon Dots ◽  
Living Cells ◽  
Ph Sensing ◽  
Ratiometric Ph Sensing

scholarly journals Optical Sensing: Ratiometric pH Sensing in Living Cells Using Carbon Dots (Part. Part. Syst. Charact. 1/2020)

2020 ◽  
Vol 37 (1) ◽  
pp. 2070002 ◽  
Author(s):  
Jun‐Ray Macairan ◽  
Issan Zhang ◽  
Adryanne Clermont‐Paquette ◽  
Rafik Naccache ◽  
Dusica Maysinger
Keyword(s):  
Carbon Dots ◽  
Optical Sensing ◽  
Living Cells ◽  
Ph Sensing ◽  
Ratiometric Ph Sensing

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

Facile synthesis of orange fluorescence carbon dots with excitation independent emission for pH sensing and cellular imaging

2018 ◽  
Vol 1042 ◽  
pp. 125-132 ◽  
Author(s):  
Yuan Jiao ◽  
Xiaojuan Gong ◽  
Hui Han ◽  
Yifang Gao ◽  
Wenjing Lu ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Cellular Imaging ◽  
Ph Sensing ◽  
Facile Synthesis ◽  
Orange Fluorescence
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