Biodetection using a ZnO nanorod-based microfluidic device with a concentration gradient generator

2020 ◽  
Vol 44 (14) ◽  
pp. 5186-5189
Author(s):  
Yan Xie ◽  
Yuchen Shi ◽  
Wenhui Xie ◽  
Mengjie Chang ◽  
Zhenjie Zhao ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Concentration Gradient ◽  
Cancer Biomarker ◽  
Zno Nanorod ◽  
Biomarker Detection ◽  
Gradient Generator

An effective microfluidic device with a concentration gradient generator for cancer biomarker detection is developed by integrating ZnO NR arrays into microchannels.

Novel enzyme-free immunomagnetic microfluidic device based on Co0.25Zn0.75Fe2O4 for cancer biomarker detection

2019 ◽  
Vol 1071 ◽  
pp. 59-69 ◽  
Author(s):  
Camila A. Proença ◽  
Thaísa A. Baldo ◽  
Tayane A. Freitas ◽  
Elsa M. Materón ◽  
Ademar Wong ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Cancer Biomarker ◽  
Biomarker Detection

Enhanced immunofluorescence detection of a protein marker using a PAA modified ZnO nanorod array-based microfluidic device

Nanoscale ◽  
2018 ◽  
Vol 10 (37) ◽  
pp. 17663-17670 ◽  
Author(s):  
Zhihua Wu ◽  
De Zhao ◽  
Chengyi Hou ◽  
Lei Liu ◽  
Jianhua Chen ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Zno Nanorods ◽  
Nanorod Array ◽  
Zno Nanorod ◽  
Biomarker Detection ◽  
Protein Marker ◽  
Zno Nanorod Array

ZnO nanorods were obtained through a controllable microfluidic reaction in capillaries and used for biomarker detection after PAA modification.

scholarly journals Finger-Actuated Microfluidic Concentration Gradient Generator Compatible with a Microplate

Micromachines ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 174 ◽  
Author(s):  
Juhwan Park ◽  
Hyewon Roh ◽  
Je-Kyun Park
Keyword(s):  
Microfluidic Device ◽  
Concentration Gradient ◽  
Volume Of Fluid ◽  
Initial Reaction Rate ◽  
Initial Reaction ◽  
Concentration Gradients ◽  
Fluidic Channel ◽  
Push Button ◽  
Gradient Generator ◽  
Wide Range

The generation of concentration gradients is an essential part of a wide range of laboratory settings. However, the task usually requires tedious and repetitive steps and it is difficult to generate concentration gradients at once. Here, we present a microfluidic device that easily generates a concentration gradient by means of push-button actuated pumping units. The device is designed to generate six concentrations with a linear gradient between two different sample solutions. The microfluidic concentration gradient generator we report here does not require external pumps because changes in the pressure of the fluidic channel induced by finger actuation generate a constant volume of fluid, and the design of the generator is compatible with the commonly used 96-well microplate. Generation of a concentration gradient by the finger-actuated microfluidic device was consistent with that of the manual pipetting method. In addition, the amount of fluid dispensed from each outlet was constant when the button was pressed, and the volume of fluid increased linearly with respect to the number of pushing times. Coefficient of variation (CV) was between 0.796% and 13.539%, and the error was between 0.111% and 19.147%. The design of the microfluidic network, as well as the amount of fluid dispensed from each outlet at a single finger actuation, can be adjusted to the user’s demand. To prove the applicability of the concentration gradient generator, an enzyme assay was performed using alkaline phosphatase (ALP) and para-nitrophenyl phosphate (pNPP). We generated a linear concentration gradient of the pNPP substrate, and the enzyme kinetics of ALP was studied by examining the initial reaction rate between ALP and pNPP. Then, a Hanes–Woolf plot of the various concentration of ALP was drawn and the Vmax and Km value were calculated.

Complete experimental and theoretical characterization of nonlinear concentration gradient generator microfluidic device for analytical purposes

2021 ◽  
Vol 189 (1) ◽  
Author(s):  
Paulo Henrique Maciel Buzzetti ◽  
Maiara Mitiko Taniguchi ◽  
Nayara de Souza Mendes ◽  
Renata Corrêa Vicentino ◽  
Jean Halison de Oliveira ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Concentration Gradient ◽  
Gradient Generator ◽  
Theoretical Characterization

scholarly journals A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3355 ◽  
Author(s):  
Wanyoung Lim ◽  
Sungsu Park
Keyword(s):  
High Throughput ◽  
Concentration Gradient ◽  
High Throughput Screening ◽  
3D Cell Culture ◽  
Drug Efficacy ◽  
Cancer Drug ◽  
Spheroid Culture ◽  
Gradient Generator ◽  
Micro Channels

Three-dimensional (3D) cell culture is considered more clinically relevant in mimicking the structural and physiological conditions of tumors in vivo compared to two-dimensional cell cultures. In recent years, high-throughput screening (HTS) in 3D cell arrays has been extensively used for drug discovery because of its usability and applicability. Herein, we developed a microfluidic spheroid culture device (μFSCD) with a concentration gradient generator (CGG) that enabled cells to form spheroids and grow in the presence of cancer drug gradients. The device is composed of concave microwells with several serpentine micro-channels which generate a concentration gradient. Once the colon cancer cells (HCT116) formed a single spheroid (approximately 120 μm in diameter) in each microwell, spheroids were perfused in the presence of the cancer drug gradient irinotecan for three days. The number of spheroids, roundness, and cell viability, were inversely proportional to the drug concentration. These results suggest that the μFSCD with a CGG has the potential to become an HTS platform for screening the efficacy of cancer drugs.

A nanostructured microfluidic device for plasmonic-assisted electrochemical detection of hydrogen peroxide released from cancer cells

Nanoscale ◽  
2021 ◽  
Author(s):  
Carolina del Real Mata ◽  
Roozbeh Siavash Moakhar ◽  
Sayed Iman Isaac Hosseini ◽  
Mahsa Jalali ◽  
Sara Mahshid
Keyword(s):  
Hydrogen Peroxide ◽  
Cancer Cells ◽  
Real Time ◽  
Electrochemical Detection ◽  
Microfluidic Device ◽  
Cancer Biomarker ◽  
Cancer Diagnostics ◽  
Liquid Biopsies ◽  
Non Invasive

Non-invasive liquid biopsies offer hope for a rapid, risk-free, real-time glimpse into cancer diagnostics. Recently, hydrogen peroxide (H2O2) is identified as a cancer biomarker due to continued release from cancer...

A radial microfluidic concentration gradient generator with high-density channels for cell apoptosis assay

Lab on a Chip ◽  
2011 ◽  
Vol 11 (19) ◽  
pp. 3305 ◽  
Author(s):  
Chun-Guang Yang ◽  
Ying-Fan Wu ◽  
Zhang-Run Xu ◽  
Jian-Hua Wang
Keyword(s):  
Concentration Gradient ◽  
Cell Apoptosis ◽  
High Density ◽  
Gradient Generator ◽  
Apoptosis Assay
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