Study on the extraction, purification, partial chemical characterization and anti-alcohol liver injury activity of Mori Fructus polysaccharides

2020 ◽  
Vol 44 (46) ◽  
pp. 20060-20070
Author(s):  
Huaguo Chen ◽  
Ruixi Xiao ◽  
Xin Zhou
Keyword(s):  
Liver Injury ◽  
Chemical Characterization ◽  
Partial Chemical

Mori Fructus (MF) is a fruit rich in many nutrients.

PURIFICATION AND PARTIAL CHEMICAL CHARACTERIZATION OF SHEEP NEUROPHYSIN

1973 ◽  
Vol 74 (2) ◽  
pp. 226-236 ◽  
Author(s):  
Michel Chrétien ◽  
Claude Gilardeau
Keyword(s):  
Amino Acid ◽  
Polyacrylamide Gel Electrophoresis ◽  
Chemical Characterization ◽  
Terminal Amino Acid ◽  
Amino Acid Determination ◽  
Pituitary Glands ◽  
Terminal Amino ◽  
Partial Chemical ◽  
Acid Determination

ABSTRACT A protein isolated from ovine pituitary glands has been purified, and its homogeneity assessed by NH2- and COOH-terminal amino acid determination, ultracentrifugation studies, and polyacrylamide gel electrophoresis after carboxymethylation. Its chemical and immunochemical properties are closely similar to those of beef and pork neurophysins, less similar to those of human neurophysins. It contains no tryptophan (like other neurophysins) or histidine (like all except bovine neurophysin-I and human neurophysins). It has alanine at the NH2-terminus and valine at the COOH-terminus. Its amino acid composition is similar to, but not identical with those of porcine and bovine neurophysins.

scholarly journals Partial chemical characterization of antigenic preparations of chromoblastomycosis agents

1999 ◽  
Vol 41 (6) ◽  
pp. 343-350 ◽  
Author(s):  
Tania Fraga BARROS ◽  
Maria Aparecida de RESENDE
Keyword(s):  
Total Lipid ◽  
Uv Light ◽  
Chemical Characterization ◽  
Total Lipids ◽  
Lipid Contents ◽  
Carbohydrate Levels ◽  
Carbohydrate Contents ◽  
Partial Chemical

Antigenic preparations (saline, methylic, metabolic and exoantigens) of four agents of chromoblastomycosis, Fonsecaea pedrosoi, Phialophora verrucosa, Cladophialophora (Cladosporium) carrionii and Rhinocladiella aquaspersa were obtained. Partial chemical characterization of these antigenic preparations was obtained by determination of the levels of total lipids, protein, and carbohydrates, and identification of the main sterols and carbohydrates. Methylic antigens presented the highest lipid contents, whereas metabolic antigens showed the highest carbohydrate content. Total lipid, protein, and carbohydrate levels were in the range of 2.33 to 2.00mg/ml, 0.04 to 0.02 mg/ml and 0.10 to 0.02 mg/ml, respectively, in the methylic antigens and in the range of 0.53 to 0.18mg/ml, 0.44 to 0.26mg/ml, and 1.82 to 1.02 mg/ml, respectively, in saline antigens. Total lipid, protein, and carbohydrate contents were in the range of 0.55 to 0.20mg/ml, 0.69 to 0.57mg/ml and 10.73 to 5.93mg/ml, respectively, in the metabolic antigens, and in the range of 0.55 to 0.15mg/ml, 0.62 to 0.20mg/ml and 3.55 to 0.42mg/ml, respectively, in the exoantigens. Phospholipids were not detected in the preparations. Saline and metabolic antigens and exoantigens presented hexose and the methylic antigen revealed additional pentose units in their composition. The UV light absorption spectra of the sterols revealed squalene and an ergosterol fraction in the antigens. The characterization of these antigenic preparations may be useful for serological evaluation of patients of chromoblastomycosis.

Isolation and partial chemical characterization of a virus-like particle from a eukaryotic alga

Virology ◽  
1981 ◽  
Vol 114 (2) ◽  
pp. 357-369 ◽  
Author(s):  
Larry H. Stanker ◽  
Larry R. Hoffman ◽  
Roderick Mac Leod
Keyword(s):  
Chemical Characterization ◽  
Eukaryotic Alga ◽  
Virus Like Particle ◽  
Partial Chemical
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