scholarly journals The effect of charge on the uptake and resistance to reduction of platinum(iv) complexes in human serum and whole blood models

Metallomics ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1599-1615
Author(s):  
Catherine K. J. Chen ◽  
Xiao Gui ◽  
Peter Kappen ◽  
Anna K. Renfrew ◽  
Trevor W. Hambley
Keyword(s):  
Human Serum ◽  
Cellular Uptake ◽  
Whole Blood ◽  
Charged Ligands

Platinum(iv) complexes can be modified by incorporating negatively charged ligands in order to inhibit cellular uptake and reduction in whole blood.

Phthalate Metabolites in Paired Human Serum and Whole Blood

2021 ◽  
Author(s):  
Weili Mao ◽  
Nan Zhao ◽  
Meirong Zhao ◽  
jin hangbiao
Keyword(s):  
Human Serum ◽  
Whole Blood ◽  
Phthalate Metabolites

scholarly journals Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

Metabolites ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 93 ◽  
Author(s):  
Cora McHugh ◽  
Thomas Flott ◽  
Casey Schooff ◽  
Zyad Smiley ◽  
Michael Puskarich ◽  
...  
Keyword(s):  
Human Serum ◽  
1H Nmr ◽  
Whole Blood ◽  
Nmr Spectra ◽  
High Quality ◽  
Healthy Human ◽  
Long Term Storage ◽  
The Impact ◽  
Term Storage

Background: Though blood is an excellent biofluid for metabolomics, proteins and lipids present in blood can interfere with 1d-1H NMR spectra and disrupt quantification of metabolites. Here, we present effective macromolecule removal strategies for serum and whole blood (WB) samples. Methods: A variety of macromolecule removal strategies were compared in both WB and serum, along with tests of ultrafiltration alone and in combination with precipitation methods. Results: In healthy human serum, methanol:chloroform:water extraction with ultrafiltration was compared to methanol precipitation with and without ultrafiltration. Methods were tested in healthy pooled human serum, and in serum from patients with sepsis. Effects of long-term storage at −80 °C were tested to explore the impact of macromolecule removal strategy on serum from different conditions. In WB a variety of extraction strategies were tested in two types of WB (from pigs and baboons) to examine the impact of macromolecule removal strategies on different samples. Conclusions: In healthy human serum methanol precipitation of serum with ultrafiltration was superior, but was similar in recovery and variance to methanol:chloroform:water extraction with ultrafiltration in pooled serum from patients with sepsis. In WB, high quality, quantifiable spectra were obtained with the use of a methanol: chloroform precipitation.

scholarly journals A Hypertransfused Mouse Assay for Thrombopoietic Factors

Blood ◽  
1973 ◽  
Vol 42 (3) ◽  
pp. 423-428 ◽  
Author(s):  
George W. Cooper ◽  
Barbara Cooper ◽  
A. Lawrence Ossias ◽  
Esmail D. Zanjani
Keyword(s):  
Human Serum ◽  
Whole Blood ◽  
Hodgkin's Disease ◽  
Healthy Donor ◽  
Hodgkin’S Disease ◽  
Normal Serum ◽  
Normal Platelet ◽  
Quantitative Separation ◽  
Thrombocytopenic Patient ◽  
Normal Human

Abstract A method was developed for the quantitative separation of platelets from CF1 mouse whole blood. This made it possible to determine the platelet incorporation of 35S-sulfate without the necessity of doing platelet counts. Daily hypertransfusions of the mice to three to four times normal platelet levels for 4-5 days significantly reduced platelet uptake of radiosulfate to an average of about 40% of the nontransfused controls. Mice rendered thrombocytopenic 48 hr earlier by antiplatelet serum, had 2-day 35S uptakes over 2 1/2 times the controls and 6 times the hypertransfused animals. The administration of a total of 2 ml of serum, given twice daily for 3 days from a thrombocytopenic patient with Hodgkin's disease caused a highly significant 103% rise in radiosulfate incorporation when compared with saline in the hypertransfused mouse. Normal human serum from a healthy donor caused a small and insignificant rise. The serum from a patient with Hodgkin's disease caused a highly significant 63% rise in 35S incorporation when compared to the normal serum.

Stability of some atypical antipsychotics in human plasma, haemolysed whole blood, oral fluid, human serum and calf serum

2013 ◽  
Vol 229 (1-3) ◽  
pp. 151-156 ◽  
Author(s):  
Danielle S. Fisher ◽  
Suzanne J. Partridge ◽  
Simon A. Handley ◽  
Robert J. Flanagan
Keyword(s):  
Human Serum ◽  
Human Plasma ◽  
Whole Blood ◽  
Oral Fluid ◽  
Atypical Antipsychotics ◽  
Calf Serum

scholarly journals Human Serum Modifies Aggregation Properties of Commonly Used Epidural Steroids

2015 ◽  
Vol 6;18 (6;11) ◽  
pp. E1131-E1138
Author(s):  
Sayed E Wahez
Keyword(s):  
Light Microscopy ◽  
Human Serum ◽  
Whole Blood ◽  
Ex Vivo ◽  
Case Reports ◽  
Care Center ◽  
Tertiary Care ◽  
Epidural Injection ◽  
Particle Aggregates ◽  
Number Of Particles

Background: Case reports of catastrophic neurological sequelae during ESIs have questioned the safety of this procedure. A proposed mechanism is particulate steroid embolization resulting in neuralischemia. Previous reports have described steroid clumping in common epidural injection mixtures. We demonstrate that physiologic medium can also modify aggregation. Objective: To inspect and compare aggregative properties of steroid preparations with and without human serum. Setting: Academic tertiary care center. Hypothesis: Particulate steroids display different aggregation characteristics in serum compared to non-physiologic solutions. Design: Solutions were inspected under light microscopy: betamethasone sodium phosphate/ betamethasone acetate, methylprednisolone, and dexamethasone were each mixed in lidocaine 1%, bupivacaine 0.5%, or sterile water in a 1:1 ratio. All preparations were inspected under light microscopy with 100x and 400x magnifications by a pathologist blinded to our expectations and hypothesis. Five random viewing fields were selected within each slide and the number of aggregates per field and the number of particles per aggregate was evaluated. Results: The addition of serum had a significant effect on steroid particle aggregation and number of particles per aggregate. Limitations: This study was limited by sample size as only 2 sets of human serum samples were tested with each preparation against one non-serum control. Additionally, as steroid preparations were evaluated under light microscopy, the ex vivo setting must be considered in the interpretation of results. Finally, mixing preparations with human serum as opposed to whole blood was necessary to allow for improved visibility on light microscopy despite the fact that whole blood may be necessary to more closely emulate in vivo coagulation setting. Conclusions: Overall, the presence of serum resulted in fewer large steroid particle aggregates when compared to non-serum control samples. Amongst particulate steroids, betamethasone with bupivacaine 0.5% demonstrated the fewest and smallest particle aggregates, suggesting that preparation may reduce the risk of embolic infarction. Methylprednisolone formed significantly larger particles in bupivicaine 0.5% with serum compared to non-serum controls. Key words: Corticosteroid, particulate aggregate, epidural injection, embolic infarction, serum

scholarly journals A high-throughput microfluidic nanoimmunoassay for detecting anti–SARS-CoV-2 antibodies in serum or ultralow-volume blood samples

2021 ◽  
Vol 118 (18) ◽  
pp. e2025289118
Author(s):  
Zoe Swank ◽  
Grégoire Michielin ◽  
Hon Ming Yip ◽  
Patrick Cohen ◽  
Diego O. Andrey ◽  
...  
Keyword(s):  
Human Serum ◽  
High Throughput ◽  
Whole Blood ◽  
Test Strip ◽  
Serum Samples ◽  
Blood Samples ◽  
Novel Technologies ◽  
Human Serum Samples ◽  
Biomarker Analysis ◽  
Glucose Test

Novel technologies are needed to facilitate large-scale detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) specific antibodies in human blood samples. Such technologies are essential to support seroprevalence studies and vaccine clinical trials, and to monitor quality and duration of immunity. We developed a microfluidic nanoimmunoassay (NIA) for the detection of anti–SARS-CoV-2 IgG antibodies in 1,024 samples per device. The method achieved a specificity of 100% and a sensitivity of 98% based on the analysis of 289 human serum samples. To eliminate the need for venipuncture, we developed low-cost, ultralow-volume whole blood sampling methods based on two commercial devices and repurposed a blood glucose test strip. The glucose test strip permits the collection, shipment, and analysis of 0.6 μL of whole blood easily obtainable from a simple finger prick. The NIA platform achieves high throughput, high sensitivity, and specificity based on the analysis of 289 human serum samples, and negligible reagent consumption. We furthermore demonstrate the possibility to combine NIA with decentralized and simple approaches to blood sample collection. We expect this technology to be applicable to current and future SARS-CoV-2 related serological studies and to protein biomarker analysis in general.

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