scholarly journals Acrylate–gelatin–carbonated hydroxyapatite (cHAP) composites for dental bone-tissue applications

2020 ◽  
Vol 1 (6) ◽  
pp. 1675-1684 ◽  
Author(s):  
Ricardas Golubevas ◽  
Zivile Stankeviciute ◽  
Aleksej Zarkov ◽  
Raimundas Golubevas ◽  
Lars Hansson ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Carbonated Hydroxyapatite ◽  
Implant Integration ◽  
Copolymerization Reaction

The favourable properties of acrylate–gelatin–cHAP composites fabricated via copolymerization reaction may make possible porosity and hydrophilicity remodelling and thus promote scaffold-implant integration.

scholarly journals Nano-carbonated hydroxyapatite precipitation from abalone shell (Haliotis asinina) waste as the bioceramics candidate for bone tissue engineering

2021 ◽  
Vol 11 ◽  
pp. 184798042110328
Author(s):  
Hestining A Permatasari ◽  
Mona Sari ◽  
Aminatun ◽  
Tri Suciati ◽  
Kiagus Dahlan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Aging Time ◽  
Carbonate Content ◽  
P Value ◽  
Transmission Electron Microscopy Analysis ◽  
Crystallinity Degree ◽  
Carbonated Hydroxyapatite ◽  
Carbonate Substitution

In this study, nano-carbonated hydroxyapatite (n-CHAp) was successfully synthesized with abalone shells ( Halioitis asinina) as the calcium source using precipitation methods with aging time variations, namely, 0 (without the aging process), 24, and 48 h. Based on an analysis of X-ray diffraction characterization, the spectrum of the n-CHAp is shown for all sample variations in aging time. The results of the calculation of lattice parameter values confirm that the phase formed is the B-type CHAp phase with the increasing crystallinity degree, crystallite size, particle size, and polydispersity which is confirmed by the presence of the CO32- functional group at 1438 cm−1 and 878 cm−1, that is, the B-type carbonate substitution characteristic. The presence of the carbonate ions identified as smaller during the extension of aging time causes the decreasing value of the Ca/P mole ratio but still has a value greater than the HAp Ca/P value (1.67), which is 1.80–1.72. Based on the transmission electron microscopy analysis, the nanometer-size of B-type CHAp particles was successfully obtained. According to the criteria for nanostructures, crystallographic properties, carbonate content, and chemical processes, B-type CHAp samples based on abalone shells ( Halioitis asinina) are one of the candidates in bioceramics for bone tissue engineering applications.

scholarly journals Sustained Calcium(II)-Release to Impart Bioactivity in Hybrid Glass Scaffolds for Bone Tissue Engineering

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1192
Author(s):  
Dzmitry Kuzmenka ◽  
Claudia Sewohl ◽  
Andreas König ◽  
Tobias Flath ◽  
Sebastian Hahnel ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bone Tissue ◽  
Culture Medium ◽  
Calcium Release ◽  
Burst Release ◽  
Cell Culture Medium ◽  
Mineral Layer ◽  
Additional Increase ◽  
Implant Integration ◽  
Ph Increase

In this study, we integrated different calcium sources into sol-gel hybrid glass scaffolds with the aim of producing implants with long-lasting calcium release while maintaining mechanical strength of the implant. Calcium(II)-release was used to introduce bioactivity to the material and eventually support implant integration into a bone tissue defect. Tetraethyl orthosilicate (TEOS) derived silica sols were cross-linked with an ethoxysilylated 4-armed macromer, pentaerythritol ethoxylate and processed into macroporous scaffolds with defined pore structure by indirect rapid prototyping. Triethyl phosphate (TEP) was shown to function as silica sol solvent. In a first approach, we investigated the integration of 1 to 10% CaCl2 in order to test the hypothesis that small CaCl2 amounts can be physically entrapped and slowly released from hybrid glass scaffolds. With 5 and 10% CaCl2 we observed an extensive burst release, whereas slightly improved release profiles were found for lower Calcium(II) contents. In contrast, introduction of melt-derived bioactive 45S5 glass microparticles (BG-MP) into the hybrid glass scaffolds as another Calcium(II) source led to an approximately linear release of Calcium(II) in Tris(hydroxymethyl)aminomethane (TRIS) buffer over 12 weeks. pH increase caused by BG-MP could be controlled by their amount integrated into the scaffolds. Compression strength remained unchanged compared to scaffolds without BG-MP. In cell culture medium as well as in simulated body fluid, we observed a rapid formation of a carbonated hydroxyapatite layer on BG-MP containing scaffolds. However, this mineral layer consumed the released Calcium(II) ions and prevented an additional increase in Calcium(II) concentration in the cell culture medium. Cell culture studies on the different scaffolds with osteoblast-like SaOS-2 cells as well as bone marrow derived mesenchymal stem cells (hMSC) did not show any advantages concerning osteogenic differentiation due to the integration of BG-MP into the scaffolds. Nonetheless, via the formation of a hydroxyapatite layer and the ability to control the pH increase, we speculate that implant integration in vivo and bone regeneration may benefit from this concept.

scholarly journals Succinylated Bacterial Cellulose Induce Carbonated Hydroxyapatite Deposition in a Solution Mimicking Body Fluid

2019 ◽  
Vol 19 (4) ◽  
pp. 858
Author(s):  
Farah Nurlidar ◽  
Mime Kobayashi
Keyword(s):  
Bacterial Cellulose ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Body Fluid ◽  
Bone Cells ◽  
Bone Tissue Regeneration ◽  
3D Scaffold ◽  
Sem Images ◽  
Carbonated Hydroxyapatite

Incorporation of bone-like hydroxyapatite into bacterial cellulose (BC) is an attractive approach for the fabrication of a bioactive three-dimensional (3D) scaffold for bone tissue regeneration. This study investigates the influence of the succinylation of BC on its ability to incorporate bone-like hydroxyapatite. A biomimetic process using a 1.5 × Simulated Body Fluid (SBF) was used to deposit the hydroxyapatite into the succinylated-BC. After soaking the succinylated-BC in the 1.5 × SBF for six days, Scanning Electron Microscope (SEM) images were taken and the composition of the succinylated-BC was analyzed by energy dispersive X-ray spectrometry. The biocompatibility of the scaffolds was tested in vitro using rat Bone Marrow Stromal Cells (rBMSCs). The SEM images and Fourier Transform Infrared Spectroscopy (FTIR) spectra showed that carbonated hydroxyapatite was deposited on the succinylated-BC. In contrast, only a small amount of carbonated hydroxyapatite deposition was observed on unmodified BC, indicating that the succinyl group in the BC is effective for inducing hydroxyapatite deposition. In vitro studies using rBMSCs revealed the biocompatibility of the scaffold. Combining with the ability of the cells to differentiate into bone cells, the succinylated-BC scaffold is a promising 3D scaffold for bone tissue regeneration.

scholarly journals Porous Carbonated Hydroxyapatite-Based Paraffin Wax Nanocomposite Scaffold for Bone Tissue Engineering: A Physicochemical Properties and Cell Viability Assay Analysis

Coatings ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1189
Author(s):  
Mona Sari ◽  
Aminatun ◽  
Tri Suciati ◽  
Yessie Widya Sari ◽  
Yusril Yusuf
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Paraffin Wax ◽  
Cell Viability Assay ◽  
Carbonated Hydroxyapatite ◽  
Viability Assay ◽  
Nanocomposite Scaffold ◽  
Nanocomposite Scaffolds

Porosity is one of the parameters of scaffold pore structure that must be developed using paraffin wax as a synthetic polymer for making porous bioceramics carbonated hydroxyapatite (CHA). This study fabricated CHA based on abalone mussel shells (Halioitis asinina); CHA/paraffin wax nanocomposite scaffolds were synthesized using paraffin wax with concentration variations of 10, 20, and 30 wt.%. The energy-dispersive X-ray spectroscopy (EDS) results showed that the Ca/P molar ratio of CHA was 1.72, which approaches the natural bone. The addition of paraffin wax in all concentration variation treatments caused the crystallographic properties of the CHA/paraffin wax nanocomposite scaffolds to decrease. The results of pore analysis suggest that the high concentration of paraffin wax in the CHA suspension is involved in the formation of more pores on the surface of the scaffold, but only CHA/paraffin wax 30 wt.% had a scaffold with potential to be used in media with a cellular growth orientation. The micropore analysis was also supported by the cell viability assay results for CHA/paraffin wax 30 wt.% nanocomposite scaffold, where serial doses of scaffold concentrations to mouse osteoblast cells were secure. Overall, based on this analysis, the CHA/paraffin wax scaffold can be a candidate for bone tissue engineering.

scholarly journals Considerations regarding the osseointegration of endosseous dental implants

2021 ◽  
Vol 67 (1) ◽  
pp. 48-52
Author(s):  
Ion Pătrașcu ◽  
◽  
Bogdan Mihai Gălbinașu ◽  
Keyword(s):  
Bone Tissue ◽  
Host Tissue ◽  
Environmental Scanning ◽  
Clinical Report ◽  
Endosseous Implants ◽  
New Type ◽  
Tissue Interface ◽  
Implant Integration ◽  
Over Time ◽  
Scanning Electron

Implant osseointegration has not been accepted over time, considering that in fact the implant integration is performed only in the soft tissue of the host. For this reason, the implant has never been sufficiently integrated into the host tissue immediately after insertion. Experiments performed in Branemark laboratories in the early 1960s, with a new type of implant, which required a direct anchorage to bone tissue for clinical function, this anchorage was called osseointegration. It has been shown that it is possible to achieve direct osseointegration if the Branemark method is considered, which was published a few years later in the first clinical report. The authors of this article come up with new contributions that validate the implant osseointegration process. Inside this article we present our methodology for evaluating the osseointegration of endosseous implants: ESEM (environmental scanning electron microscope) studies of the implant-bone tissue interface.

Sternal augmentation plasty utilizing homologous bone tissue after Fontan operation

2010 ◽  
Vol 58 (S 01) ◽  
Author(s):  
W Kuroczynski ◽  
C Kampmann ◽  
R Huth ◽  
M Hartert ◽  
M Heinemann ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Fontan Operation
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