scholarly journals Three-dimensional visualization and analysis of flowing droplets in microchannels using real-time quantitative phase microscopy

Lab on a Chip ◽  
2021 ◽  
Vol 21 (1) ◽  
pp. 75-82
Author(s):  
Yingdong Luo ◽  
Jinwu Yang ◽  
Xinqi Zheng ◽  
Jianjun Wang ◽  
Xin Tu ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Three Dimensional ◽  
Phase Variation ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy ◽  
On Chip

We present real-time quantitative phase microscopy (RT-QPM) that can be used for on-chip three-dimensional visualization of droplets and high-throughput quantitative molecular measurement via real-time extraction of sample-induced phase variation.

scholarly journals High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Azeem Ahmad ◽  
Vishesh Dubey ◽  
Nikhil Jayakumar ◽  
Anowarul Habib ◽  
Ankit Butola ◽  
...  
Keyword(s):  
Light Source ◽  
High Throughput ◽  
High Speed ◽  
Light Sources ◽  
Single Shot ◽  
Phase Sensitivity ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Spatial Phase ◽  
Quantitative Phase Microscopy

AbstractHigh space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative phase microscopy (QPM) system. It opens avenue for widespread applications of QPM in the field of biomedical imaging. Temporally low coherence light sources are implemented to achieve high spatial phase sensitivity in QPM at the cost of either reduced temporal resolution or smaller field of view (FOV). In addition, such light sources have low photon degeneracy. On the contrary, high temporal coherence light sources like lasers are capable of exploiting the full FOV of the QPM systems at the expense of less spatial phase sensitivity. In the present work, we demonstrated that use of narrowband partially spatially coherent light source also called pseudo-thermal light source (PTLS) in QPM overcomes the limitations of conventional light sources. The performance of PTLS is compared with conventional light sources in terms of space bandwidth product, phase sensitivity and optical imaging quality. The capabilities of PTLS are demonstrated on both amplitude (USAF resolution chart) and phase (thin optical waveguide, height ~ 8 nm) objects. The spatial phase sensitivity of QPM using PTLS is measured to be equivalent to that for white light source and supports the FOV (18 times more) equivalent to that of laser light source. The high-speed capabilities of PTLS based QPM is demonstrated by imaging live sperm cells that is limited by the camera speed and large FOV is demonstrated by imaging histopathology human placenta tissue samples. Minimal invasive, high-throughput, spatially sensitive and single-shot QPM based on PTLS will enable wider penetration of QPM in life sciences and clinical applications.

Real time quantitative phase microscopy based on single-shot transport of intensity equation (ssTIE) method

2016 ◽  
Vol 109 (7) ◽  
pp. 071112 ◽  
Author(s):  
Wei Yu ◽  
Xiaolin Tian ◽  
Xiaoliang He ◽  
Xiaojun Song ◽  
Liang Xue ◽  
...  
Keyword(s):  
Real Time ◽  
Single Shot ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy ◽  
Transport Of Intensity Equation

Lab on chip optical imaging of biological sample by quantitative phase microscopy

2015 ◽  
Author(s):  
P. Memmolo ◽  
L. Miccio ◽  
F. Merola ◽  
O. Gennari ◽  
M. Mugnano ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Biological Sample ◽  
Lab On Chip ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy ◽  
On Chip

Red blood cell three-dimensional morphometry by quantitative phase microscopy

2015 ◽  
Author(s):  
F. Merola ◽  
P. Memmolo ◽  
L. Miccio ◽  
O. Gennari ◽  
M. Mugnano ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Three Dimensional ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy

High-throughput label-free screening of euglena gracilis with optofluidic time-stretch quantitative phase microscopy

2017 ◽  
Author(s):  
Baoshan Guo ◽  
Cheng Lei ◽  
Takuro Ito ◽  
Yalikun Yaxiaer ◽  
Hirofumi Kobayashi ◽  
...  
Keyword(s):  
High Throughput ◽  
Euglena Gracilis ◽  
Label Free ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy
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