A chip device to determine surface charge properties of confluent cell monolayers by measuring streaming potential

Lab on a Chip ◽  
2020 ◽  
Vol 20 (20) ◽  
pp. 3792-3805 ◽  
Author(s):  
András Kincses ◽  
Ana R. Santa-Maria ◽  
Fruzsina R. Walter ◽  
László Dér ◽  
Nóra Horányi ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Streaming Potential ◽  
Cell Monolayers ◽  
Biological Barriers ◽  
Cell Surface Charge ◽  
Cell Layers ◽  
Confluent Cell

Chip device to monitor streaming potential of confluent cell layers reflecting cell surface charge important for the function of biological barriers.

scholarly journals Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Cell Surface ◽  
Charge Density ◽  
Surface Charge ◽  
Charge Distribution ◽  
Surface Charge Density ◽  
Single Cells ◽  
Fluorescent Light ◽  
Fluorescent Nanoparticles ◽  
Cell Surface Charge ◽  
Surface Charge Distribution

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.

Bio-fabricated silver nanoparticles preferentially targets Gram positive depending on cell surface charge

2016 ◽  
Vol 83 ◽  
pp. 548-558 ◽  
Author(s):  
Debasis Mandal ◽  
Sandeep Kumar Dash ◽  
Balaram Das ◽  
Sourav Chattopadhyay ◽  
Totan Ghosh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Surface ◽  
Surface Charge ◽  
Gram Positive ◽  
Cell Surface Charge

Regulation of bacterial abstraction of lead by cell surface charge and chemical equilibria

Chemosphere ◽  
1980 ◽  
Vol 9 (1) ◽  
pp. 21-31 ◽  
Author(s):  
Christopher L. Haber ◽  
Thomas G. Tornabene ◽  
R.K. Skogerboe
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Chemical Equilibria ◽  
Cell Surface Charge

Effects of Cell Surface Charge and Hydrophobicity on Attachment of 16 Salmonella Serovars to Cantaloupe Rind and Decontamination with Sanitizers†

2006 ◽  
Vol 69 (8) ◽  
pp. 1835-1843 ◽  
Author(s):  
DIKE O. UKUKU ◽  
WILLIAM F. FETT
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Surface ◽  
Surface Charge ◽  
Storage Period ◽  
Bacterial Attachment ◽  
The Other ◽  
Average Percentage ◽  
Log Reduction ◽  
Cell Surface Charge ◽  
Salmonella Serovars

Adherence of bacteria to cantaloupe rind is favored by surface irregularities such as roughness, crevices, and pits, thus reducing the ability of washing or sanitizer treatments to remove or inactivate attached cells. In this study, we compared the surface charge and hydrophobicity of two cantaloupe-related outbreak strains of Salmonella Poona (RM2350 and G-91-1595) to those of 14 additional Salmonella strains using electrostatic and hydrophobic interaction chromatography. The relative abilities of the 16 strains to attach to cantaloupe surfaces and resist removal by washing with water, chlorine (200 ppm), or hydrogen peroxide (2.5%) for 5 min after a storage period of up to 7 days at 5 to 20°C also were determined. Whole cantaloupes were inoculated with each pathogen at 8.36 log CFU/ml, dried for 1 h inside a biosafety cabinet, stored, and then subjected to the washing treatments. Only the positive surface charge of the two cantaloupe-related strains of Salmonella Poona was significantly higher (P < 0.05) than that of the other strains. Initial bacterial attachment to cantaloupe surfaces ranged from 3.68 to 4.56 log CFU/cm2 (highest values for Salmonella Michigan, Newport, Oranienburg, and Mbandaka). The average percentage of the total bacterial population strongly attached to the cantaloupe surface for the Salmonella serovars studied ranged from 0.893 to 0.946 at 5°C and from 0.987 to 0.999 at 25°C. Washing inoculated melons with water did not produce a significant reduction in the concentration of the pathogens (P > 0.05). Chlorine and hydrogen peroxide treatments caused an average 3-log reduction when applied 20 to 40 min postinoculation. However, sanitizer treatments applied 60 min or more postinoculation were less effective (approximately 2.5-log reduction). No significant differences were noted in sanitizer efficacy against the individual strains (P > 0.05). The two cantaloupe-related outbreak Salmonella Poona strains did not significantly differ from the other Salmonella strains tested in negative cell surface charge or hydrophobicity, were not more effective in attaching to whole melon surfaces, and were not more resistant to the various washing treatments when present on rinds.

scholarly journals Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the innate immune system

2019 ◽  
Vol 15 (5) ◽  
pp. e1007730 ◽  
Author(s):  
Robert E. Smith ◽  
Bartłomiej Salamaga ◽  
Piotr Szkuta ◽  
Natalia Hajdamowicz ◽  
Tomasz K. Prajsnar ◽  
...  
Keyword(s):  
Immune System ◽  
Cell Surface ◽  
Surface Charge ◽  
Innate Immune System ◽  
Innate Immune ◽  
Polysaccharide Antigen ◽  
Cell Surface Charge

scholarly journals Tuning cell surface charge in E. coli with conjugated oligoelectrolytes

2016 ◽  
Vol 7 (3) ◽  
pp. 2023-2029 ◽  
Author(s):  
Chelsea Catania ◽  
Alexander W. Thomas ◽  
Guillermo C. Bazan
Keyword(s):  
Zeta Potential ◽  
Cell Surface ◽  
Surface Charge ◽  
E Coli ◽  
Cell Surface Charge

Conjugated oligoelectrolytes intercalate into and associate with membranes, thereby changing the surface charge of microbes, as determined by zeta potential measurements.

Nanosecond pulsed electric field induced changes in cell surface charge density

Micron ◽  
2017 ◽  
Vol 100 ◽  
pp. 45-49 ◽  
Author(s):  
Diganta Dutta ◽  
Xavier-Lewis Palmer ◽  
Anthony Asmar ◽  
Michael Stacey ◽  
Shizhi Qian
Keyword(s):  
Cell Surface ◽  
Electric Field ◽  
Charge Density ◽  
Surface Charge ◽  
Surface Charge Density ◽  
Pulsed Electric Field ◽  
Cell Surface Charge ◽  
Nanosecond Pulsed Electric Field ◽  
Induced Changes
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