scholarly journals Multiplexed end-point microfluidic chemotaxis assay using centrifugal alignment

Lab on a Chip ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 3096-3103
Author(s):  
Sampath Satti ◽  
Pan Deng ◽  
Kerryn Matthews ◽  
Simon P. Duffy ◽  
Hongshen Ma
Keyword(s):  
High Throughput ◽  
Cell Alignment ◽  
Chemotaxis Assay ◽  
End Point

We used centrifugal cell alignment to develop a multiplexed end-point chemotaxis assay scalable for high-throughput applications.

An automated, high-throughput, 384 well Cytochrome P450 cocktail IC50 assay using a rapid resolution LC–MS/MS end-point

2008 ◽  
Vol 48 (1) ◽  
pp. 92-99 ◽  
Author(s):  
Kuresh A. Youdim ◽  
Richard Lyons ◽  
Leon Payne ◽  
Barry C. Jones ◽  
Kenneth Saunders
Keyword(s):  
Cytochrome P450 ◽  
High Throughput ◽  
Rapid Resolution ◽  
End Point

A quantitative high-throughput chemotaxis assay using bioluminescent reporter cells

2005 ◽  
Vol 302 (1-2) ◽  
pp. 78-89 ◽  
Author(s):  
Reena P. Vishwanath ◽  
Christine E. Brown ◽  
Jamie R. Wagner ◽  
Hunsar B. Meechoovet ◽  
Araceli Naranjo ◽  
...  
Keyword(s):  
High Throughput ◽  
Chemotaxis Assay

scholarly journals Ligand-Mediated Receptor Assembly as an End Point for High-Throughput Chemical Toxicity Screening

2017 ◽  
Vol 51 (16) ◽  
pp. 9327-9333 ◽  
Author(s):  
Elizabeth K. Medlock Kakaley ◽  
Stephanie A. Eytcheson ◽  
Gerald A. LeBlanc
Keyword(s):  
High Throughput ◽  
Toxicity Screening ◽  
Chemical Toxicity ◽  
End Point ◽  
Receptor Assembly

scholarly journals A High-throughput End-point Assay for Viable Mammalian Cell Estimation

2005 ◽  
Vol 49 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Xianghui Gong ◽  
Qiangyi Fang ◽  
Xuesen Li ◽  
Xiangzong Han ◽  
Yuyin Wu ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
High Throughput ◽  
End Point

scholarly journals Multiplexed End-point Microfluidic Chemotaxis Assay using Centrifugal Alignment

2020 ◽  
Author(s):  
Sampath Satti ◽  
Pan Deng ◽  
Kerryn Matthews ◽  
Simon P. Duffy ◽  
Hongshen Ma
Keyword(s):  
Fluid Flow ◽  
Drug Testing ◽  
Time Lapse ◽  
Cell Alignment ◽  
Cell Geometry ◽  
End Point ◽  
Starting Point ◽  
Correct Location ◽  
Set Up ◽  
Time Lapse Imaging

AbstractA fundamental challenge to multiplexing microfluidic chemotaxis assays at scale is the requirement for time-lapse imaging to continuously track migrating cells. Drug testing and drug screening applications require the ability to perform hundreds of experiments in parallel, which is not feasible for assays that require continuous imaging. To address this limitation, end-point chemotaxis assays have been developed using fluid flow to align cells in traps or sieves prior to cell migration. However, these methods require precisely controlled fluid flow to transport cells to the correct location without undesirable mechanical stress, which introduce significant set up time and design complexity. Here, we describe a microfluidic device that eliminates the need for precise flow control by using centrifugation to align cells at a common starting point. A chemoattractant gradient is then formed using passive diffusion prior to chemotaxis in an incubated environment. This approach provides a simple and scalable approach to multiplexed chemotaxis assays. Centrifugal alignment is also insensitive to cell geometry, enabling this approach to be compatible with primary cell samples that are often heterogeneous. We demonstrate the capability of this approach by assessing chemotaxis of primary neutrophils in response to an fMLP (N-formyl-met-leu-phe) gradient. Our results show that cell alignment by centrifugation offers a potential avenue to develop scalable end-point multiplexed microfluidic chemotaxis assays.

A microgroove patterned multiwell cell culture plate for high-throughput studies of cell alignment

2014 ◽  
Vol 111 (12) ◽  
pp. 2537-2548 ◽  
Author(s):  
Petra B. Lücker ◽  
Sahar Javaherian ◽  
John P. Soleas ◽  
Duncan Halverson ◽  
Peter W. Zandstra ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Cell Alignment ◽  
Culture Plate

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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