A lab-on-chip device for the sample-in-result-out detection of viable Salmonella using loop-mediated isothermal amplification and real-time turbidity monitoring

Lab on a Chip ◽  
2020 ◽  
Vol 20 (13) ◽  
pp. 2296-2305 ◽  
Author(s):  
Siyuan Wang ◽  
Ning Liu ◽  
Lingyan Zheng ◽  
Gaozhe Cai ◽  
Jianhan Lin
Keyword(s):  
Real Time ◽  
Foodborne Pathogens ◽  
Food Poisoning ◽  
Isothermal Amplification ◽  
Rapid Screening ◽  
Lab On Chip ◽  
Loop Mediated Isothermal Amplification ◽  
On Chip

Rapid screening of foodborne pathogens is key to prevent food poisoning.

On-chip parallel detection of foodborne pathogens using loop-mediated isothermal amplification

2013 ◽  
Vol 15 (5) ◽  
pp. 821-830 ◽  
Author(s):  
Carlos Duarte ◽  
Eric Salm ◽  
Brian Dorvel ◽  
Bobby Reddy ◽  
Rashid Bashir
Keyword(s):  
Foodborne Pathogens ◽  
Isothermal Amplification ◽  
Parallel Detection ◽  
Loop Mediated Isothermal Amplification ◽  
On Chip

scholarly journals Rapid Screening for Salmonella in Raw Pet Food by Loop-Mediated Isothermal Amplification

2020 ◽  
Author(s):  
Kelly J. Domesle ◽  
Shenia R. Young ◽  
Beilei Ge
Keyword(s):  
Foodborne Pathogens ◽  
Food Samples ◽  
Isothermal Amplification ◽  
Rapid Screening ◽  
Screening Methods ◽  
Lamp Method ◽  
Pet Food ◽  
Loop Mediated Isothermal Amplification ◽  
Freeze Dried ◽  
Antimicrobial Resistance Genes

Raw pet food, comprised of raw meat and vegetables, has increased in popularity in recent years. Multiple surveys and frequent recalls indicate that this commodity has a high risk of contamination with Salmonella and other foodborne pathogens. Improved screening methods are needed to meet the growing demand for testing. This matrix verification study aimed to apply a Salmonella loop-mediated isothermal amplification (LAMP) method, recently completed multi-laboratory validation in dry dog food, in several raw pet food matrices, following the U.S. Food and Drug Administration’s method validation guidelines. Five types of raw pet food, consisting of freeze-dried beef and chicken treats, and frozen beef, pork, and turkey complete foods, were evaluated. For each matrix, two sets of ten 25-g test portions (seven inoculated with ≤ 30 cells of Salmonella Typhimurium and three uninoculated controls) were examined. One set was preenriched in buffered peptone water and the other one in lactose broth, which was followed by LAMP screening using two isothermal master mixes (ISO-001 and ISO-004). All results were confirmed by culture as specified in the Bacteriological Analytical Manual (BAM). The LAMP method accurately detected Salmonella in all inoculated test portions of the five raw pet food samples, regardless of the preenrichment broth used. Positive results could be obtained within 4 min of the LAMP run using the LAMP ISO-004 master mix. All uninoculated controls tested negative by LAMP or BAM. Additionally, one turkey-based complete pet food sample was found to be already contaminated with three Salmonella serovars harboring multiple antimicrobial resistance genes. The Salmonella LAMP method offers a rapid, reliable, and robust tool for routine screening of Salmonella in raw pet food, which will help better ensure product safety and protect public health.

scholarly journals A one-step rapid screening test ofListeria monocytogenesin food samples using a real-time loop-mediated isothermal amplification turbidity assay

2017 ◽  
Vol 9 (45) ◽  
pp. 6403-6410 ◽  
Author(s):  
Sirirat Wachiralurpan ◽  
Thayat Sriyapai ◽  
Supatra Areekit ◽  
Pichapak Sriyapai ◽  
Dueantem Thongphueak ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Real Time ◽  
Screening Test ◽  
Food Samples ◽  
Isothermal Amplification ◽  
Rapid Screening ◽  
Loop Mediated Isothermal Amplification ◽  
Rapid Screening Test ◽  
One Step ◽  
Time Loop

A rapid and specific,hly-based, loop-mediated isothermal amplification (LAMP) was applied for the detection ofListeria monocytogenesin food samples using LAMP-turbidity.

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