scholarly journals 3D nanoplasmonic biosensor for detection of filopodia in cells

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2188-2196 ◽  
Author(s):  
Shuyan Zhu ◽  
Mohammed A. Eldeeb ◽  
Stella W. Pang
Keyword(s):  
Cell Membrane ◽  
In Cells

Filopodia detection using nanoplasmonic biosensors, where microposts were used to separate the cell membrane from filopodia and 3D nanopillars were used to monitor nanometer-sized filopodia.

scholarly journals Changes in Cell Membrane and Cellular Lipids in Apoptotic Cells Induced by Dolichyl Phosphate Differ from Findings in Cells Induced by Etoposide.

2002 ◽  
Vol 51 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Akiko HORIUCHI ◽  
Etsuko YASUGI ◽  
Chizu IWASAKI ◽  
Keiji FUJIMOTO ◽  
Mieko OSHIMA
Keyword(s):  
Cell Membrane ◽  
Apoptotic Cells ◽  
Dolichyl Phosphate ◽  
Cellular Lipids ◽  
In Cells

scholarly journals THE PHOTOSYNTHETIC APPARATUS OF RHODOSPIRILLUM MOLISCHIANUM

1965 ◽  
Vol 26 (2) ◽  
pp. 395-412 ◽  
Author(s):  
Sarah P. Gibbs ◽  
W. R. Sistrom ◽  
Patricia B. Worden
Keyword(s):  
Cell Membrane ◽  
Photosynthetic Apparatus ◽  
Variable Number ◽  
Electron Microscopic ◽  
Microscopic Appearance ◽  
Whole Cells ◽  
Cell Extracts ◽  
Internal Membrane ◽  
Rhodospirillum Molischianum ◽  
In Cells

By varying the light intensity and temperature during growth it is possible to obtain cultures of Rhodospirillum molischianum in which the specific bacteriochlorophyll contents differ by as much as fivefold. We used such cultures to compare the changes in the electron microscopic appearance of the cells with the changes in the amount and bacteriochlorophyll content of chromatophore material isolated from cell extracts. The cells contained a variable number of internal membranes which are invaginations of the cell membrane. The shape, size, number, and arrangement of the infoldings varied as the specific bacteriochlorophyll content of the cells changed. In cells with little bacteriochlorophyll, the invaginations were mostly tubular. In cells with larger amounts of bacteriochlorophyll, the invaginations were disc-shaped and the discs were appressed together in stacks of 2 to 10 discs each. Variations in the number of discs per stack could be accounted for by a simple statistical model. The average area per disc increased with increasing bacteriochlorophyll content. Quantitative estimations of the relative volumes occupied by membranes in cells with four different bacteriochlorophyll contents showed that the amount of internal membrane alone had no direct relationship with the bacteriochlorophyll content of the cells; however, the total amount of membrane (cell membrane plus internal membrane) was directly proportional to the bacteriochlorophyll content. The specific bacteriochlorophyll content of isolated chromatophore material was proportional to the bacteriochlorophyll content of whole cells; the total amount of chromatophore material was independent of the bacteriochlorophyll content of whole cells. Several possible explanations of this paradoxical discrepancy between the electron microscope observations and the analytical results are discussed.

Oxytocin-induced changes in single cell K+ currents and smooth muscle contraction of guinea-pig gastric antrum

1997 ◽  
Vol 136 (5) ◽  
pp. 531-538 ◽  
Author(s):  
Dessislava B Duridanova ◽  
Milena D Nedelcheva ◽  
Hristo S Gagov
Keyword(s):  
Smooth Muscle ◽  
Cell Membrane ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Gastric Antrum ◽  
Dependent Manner ◽  
Response Curves ◽  
Dose Dependent ◽  
In Cells

Abstract To study the effects of oxytocin on both spontaneous phasic contractions and K+ outward currents (IK) of the so-called 'non-target' smooth muscle cells, physiological concentrations of oxytocin ranging between 10−12 mol/l and 10−8 mol/l were applied to smooth muscle preparations and single voltage-clamped cells isolated from the circular layer of the guinea-pig gastric antrum. Oxytocin (10−12mol/l to 10−8 mol/l) suppressed, in a dose-dependent manner, the tetrodotoxin- and atropine-resistant spontaneous phasic contractions and shifted rightward the dose–response curves of 10−7 mol/l charybdotoxin and 10−3mol/l BaCl2. In cells with preloaded intracellular Ca2+ stores, oxytocin (10−12 mol/l to 10−9 mol/l) caused a dose-dependent activation of the charybdotoxin-blockable non-inactivating component of IK (IK(s1)) of single voltage-clamped cells, which was accompanied by hyperpolarization of the cell membranes. 8Lys-vasopressin and 8arg-vasopressin failed to mimic the effects of oxytocin on both contraction and K+ currents. Further, the oxytocin-induced activation of IK(s1) was effectively antagonized by 5× 10−8 mol/l U-73122 or 5× 10−6 mol/l 2-nitro-4-carboxyphenyl N,N-diphenylcarbamate (inhibitors of the cell membrane phospholipase C), as well as by intracellularly applied heparin (selective inhibitor of inositol-1,4,5-trisphosphate (IP3)-induced Ca2+ release channels). In cells incubated in the absence of Ca2+ entry throughout the study, oxytocin (10−9 mol/l) caused a slight and transient increase of IK(s1) amplitudes. Neither ryanodine (10−6 mol/l nor cyclopiazonic acid (10−6 mol/l) were able to restore the IK-activating effect of oxytocin in these cells. The data obtained suggest (i) that selective oxytocin receptors are present on the membranes of guinea-pig antral smooth muscle cells, (ii) that the oxytocin-related relaxation may result from the activation of Ca2+-sensitive K+ conductivity via activation of IP3-induced release of Ca from the submembrane located cisternae of the sarcoplasmic reticulum Ca2+ stores and (iii) in turn, this evokes a non-inactivating component of IK, hyperpolarizing the cell membrane. European Journal of Endocrinology 136 531–538

Distribution of ytterbium (Yb) in cells of Streptomyces sp. YB-1 which can accumulate Yb, and reusability of cells and cell membrane as bioadsorbent for Yb

2004 ◽  
Vol 98 (3) ◽  
pp. 214-216 ◽  
Author(s):  
Ambar Pertiwiningrum ◽  
Yoshinaka Ino ◽  
Tohru Suzuki ◽  
Tomonori Iwama ◽  
Keiichi Kawai
Keyword(s):  
Cell Membrane ◽  
Streptomyces Sp ◽  
In Cells

scholarly journals Bradykinin-induced oscillations of cell membrane potential in cells expressing the Ha-ras oncogene.

1991 ◽  
Vol 266 (8) ◽  
pp. 4938-4942
Author(s):  
F Lang ◽  
F Friedrich ◽  
E Kahn ◽  
E Wöll ◽  
M Hammerer ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Cell Membrane ◽  
Cell Membrane Potential ◽  
Ras Oncogene ◽  
In Cells

scholarly journals Murine leukemia virus transmembrane protein R-peptide is found in small virus core-like complexes in cells

2006 ◽  
Vol 87 (6) ◽  
pp. 1583-1588 ◽  
Author(s):  
Klaus Bahl Andersen ◽  
Huong Ai Diep ◽  
Anne Zedeler
Keyword(s):  
Cell Membrane ◽  
Murine Leukemia Virus ◽  
Transmembrane Protein ◽  
Leukemia Virus ◽  
Envelope Proteins ◽  
Viral Envelope ◽  
Virus Core ◽  
Small Virus ◽  
Murine Leukemia ◽  
In Cells

The core of the retrovirus Murine leukemia virus (MLV) consists of the Gag precursor protein and viral RNA. It assembles at the cytoplasmic face of the cell membrane where, by an unclear mechanism, it collects viral envelope proteins embedded in the cell membrane and buds off. The C-terminal half of the short cytoplasmic tail of the envelope transmembrane protein (TM) is cleaved off to yield R-peptide and fusion-active TM. In Moloney MLV particles, R-peptide was found to bind to core particles. In cells, R-peptide and low amounts of uncleaved TM were found to be associated with small core-like complexes, i.e. mild detergent-insoluble, Gag-containing complexes with a density of 1.23 g ml−1 and a size of 150–200 S. Our results suggest that TM associates with the assembling core particle through the R-peptide before budding and that this is the mechanism by which the budding virus acquires the envelope proteins.

Water and electrolyte contents, cell pH and membrane potentials of cultured turtle thyroid cells

1985 ◽  
Vol 104 (1) ◽  
pp. 45-52 ◽  
Author(s):  
S. Y. Chow ◽  
Y. C. Yen-Chow ◽  
D. M. Woodbury
Keyword(s):  
Membrane Potential ◽  
Cell Membrane ◽  
Culture Medium ◽  
Resting Membrane Potential ◽  
Follicular Cells ◽  
Iodide Uptake ◽  
Thyroid Cells ◽  
Cell Ph ◽  
Cells Cultured ◽  
In Cells

ABSTRACT Water and electrolyte contents, cell pH, membrane potential and 125I− uptake were determined in cultured follicular cells of turtle thyroid. The Na+, K+ and Cl− concentrations in the cultured thyroid cells were 59·2, 119·0 and 50·9 mmol/l cell water respectively. Treatment with TSH (10 mu./ml for 24 h) increased the K+ and Cl− and decreased the Na+ concentrations in cells. The water and protein contents of these cells were 81·6 and 8·7 g/100 g cells respectively. The cell pH was 6·91. With glass microelectrodes, the resting membrane potential of thyroid cells cultured in Medium 199 averaged 33·9 ± 0·63 mV which is slightly higher than 29·8 ± 1·6 mV as calculated from the data on the uptakes of [14C]methyltriphenylphosphonium and 3H2O by the cells. The potential varied linearly with the log of external K + concentration (between 15 and 120 mmol/l) with a slope of about 24 mV per tenfold change in K+ concentration. Both TSH and cyclic AMP depolarized the cell membrane. Calculations based on the values for the electrolyte concentrations in cells and in culture medium indicated that Na+, K+ and Cl− were not distributed according to their electrochemical gradients across the cell membrane. Na+ was actively transported out of the cells and K+ and Cl− into the cells. Follicular cells of turtle thyroid cultured in the medium without addition of TSH formed a monolayer. Their iodide-concentrating ability was low and they did not respond to TSH with an increase in iodide uptake. In contrast, cells cultured in medium containing TSH tended to aggregate and organize to form follicles. They had higher ability to concentrate iodide and respond to TSH. J. Endocr. (1985) 104, 45–52

scholarly journals Intracellular pH regulation in freshly isolated suspensions of rabbit inner medullary collecting duct cells: role of Na+:H+ antiporter and H(+)-ATPase.

1990 ◽  
Vol 1 (6) ◽  
pp. 890-901
Author(s):  
D Kikeri ◽  
M L Zeidel
Keyword(s):  
Membrane Potential ◽  
Cell Membrane ◽  
Intracellular Ph ◽  
Recovery Rate ◽  
Collecting Duct ◽  
Atp Depletion ◽  
Cell Membrane Potential ◽  
Inner Medullary Collecting Duct ◽  
Medullary Collecting Duct ◽  
In Cells

To define proton transport mechanisms involved in the regulation of intracellular pH (pHi) in cells of the inner medullary collecting duct (IMCD), pHi and cell membrane potential were estimated by using the fluorescent dyes 2,7-biscarboxyethyl-5(6)-carboxyfluorescein and 3,3'-dipropylthiadicarbocyanine iodide, respectively, in suspensions of freshly isolated rabbit IMCD cells. The resting pHi of IMCD cells in nonbicarbonate Ringer's solution (pH 7.4) was 7.21 +/- 0.03 (mean +/- SE). When cells were acidified by ammonium withdrawal, the initial pHi recovery rate was 0.33 +/- 0.02 pH unit/min; replacement of extracellular Na+ (130 mM) with N-methyl-D-glucamine+ reduced the pHi recovery rate to 0.08 +/- 0.02 pH unit/min, while addition of 0.1 mM amiloride in the presence of extracellular Na+ reduced the rate of pHi recovery to 0.02 +/- 0.02 pH unit/min. Similar results were obtained in cells acid loaded with HCl. Cells recovering from acidification exhibited 22Na+ uptake rates threefold higher than did nonacidified cells. The rate of Na(+)-dependent pHi recovery was independent of the cell membrane potential. In the absence of extracellular Na+, depolarizing cell membrane potential in a stepwise manner by increasing extracellular K+ concentrations from 1 to 130 mM resulted in graded increments in the rate of pHi recovery. In the presence of 130 mM K+, the pHi recovery rate in acidified cells was dependent on cellular ATP levels, sensitive to 1 mM N-ethylmaleimide, and insensitive to 0.01 mM oligomycin in the presence of glucose (control, 0.24 +/- 0.01; ATP-depleted, 0.13 +/- 0.02; addition of N-ethylmaleimide, 0.16 +/- 0.01; addition of oligomycin, 0.27 +/- 0.02 pH unit/min). ATP depletion markedly inhibited H+ extrusion from IMCD cells measured by using a pH stat. These results provide direct evidence in freshly isolated IMCD cells that both a Na+:H+ antiporter and a rheogenic H(+)-ATPase participate in pHi regulation.

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