Comprehensive utilization strategy of cellulose in a facile, controllable, high-yield preparation process of cellulose nanocrystals using aqueous tetrabutylphosphonium hydroxide

2021 ◽  
Author(s):  
Fangchao Cheng ◽  
Panpan Zhao ◽  
Tulong Ouyang ◽  
Jianping Sun ◽  
Yiqiang Wu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Cellulose Nanocrystals ◽  
High Yield ◽  
Utilization Rate ◽  
Preparation Process ◽  
Comprehensive Utilization ◽  
High Utilization ◽  
Hydrolysis Of ◽  
Utilization Strategy

Tetrabutylphosphonium hydroxide treatment of cellulose enabled facile, controllable, high-yield preparation of cellulose nanocrystals and effective enzymatic hydrolysis of cellulosic residue, resulting in a high utilization rate of more than 95%.

Production with a High Yield of Bacterial Cellulose Nanocrystals by Enzymatic Hydrolysis

2019 ◽  
Vol 19 (03) ◽  
pp. 1950015
Author(s):  
Ricardo Brandes ◽  
Leticia de Souza ◽  
Claudimir Carminatti ◽  
Derce Recouvreux
Keyword(s):  
Enzymatic Hydrolysis ◽  
Bacterial Cellulose ◽  
Cellulose Nanocrystals ◽  
Primary Objective ◽  
High Yield ◽  
Low Density ◽  
Enzymatic Method ◽  
Citrate Buffer ◽  
Cellulose Hydrogel ◽  
Time Required

Bacterial cellulose nanocrystals are highly crystalline structures with nanoscopic scale dimensions that have received increased attention in the nanocomposites area. Its properties, such as large surface area, low density, mechanical strength and ease of modification, are attractive to the preparation many kinds of nanomaterials applied multifunctional in various fields. Besides, the cellulose nanocrystals are from abundant and renewable sources that are biodegradable. An altemative method is to obtain bacterial cellulose nanocrystal by enzymatic hydrolysis because it is, less expensive, it does not use chemicals and it requires much less energy. In this sense, the primary objective of this study was to produce bacterial cellulose using glycerol as a carbon source and isolate nanocrystals from bacterial cellulose using the enzymatic hydrolysis. This study also investigated the yield of nanocrystals depending on the weight of the bacterial cellulose hydrogel, keeping constant some enzymes. The study shows us that the enzymatic method has the best performance when using cellulose hydrogel 2[Formula: see text]g to 40[Formula: see text][Formula: see text]L cellulase enzyme (endoglucanase) and 1[Formula: see text]mL of citrate buffer. Also, it was observed that the yield of nanocrystals decrease with increasing time required for the hydrolysis.

Using recyclable pH-responsive lignin amphoteric surfactant to enhance the enzymatic hydrolysis of lignocelluloses

2017 ◽  
Vol 19 (22) ◽  
pp. 5479-5487 ◽  
Author(s):  
Cheng Cai ◽  
Xuejuan Zhan ◽  
Meijun Zeng ◽  
Hongming Lou ◽  
Yuxia Pang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Amphoteric Surfactant ◽  
Ph Responsive ◽  
Comprehensive Utilization ◽  
Hydrolysis Of

Adding recyclable pH-responsive lignin amphoteric surfactant (SLQA) can enhance enzymatic hydrolysis and enable the comprehensive utilization of lignocelluloses.

scholarly journals Optimization of the Process of Compound Enzymatic Hydrolysis of Soluble Protease Preparation

2019 ◽  
Vol 131 ◽  
pp. 01024
Author(s):  
Hao Liu ◽  
Chunhui Lv ◽  
Peng Liu ◽  
Lihui Wang ◽  
Peng Wang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Membrane Protein ◽  
Experimental Method ◽  
Utilization Rate ◽  
Main Research ◽  
Agriculture Industry ◽  
Egg Membrane ◽  
Hydrolysis Of

soluble protease preparations, including trypsin, papain and other preparations, are widely used in various fields, such as medicine, agriculture, industry and so on. Based on this, this paper takes the soluble protease as the main research object, and uses the experimental method of compound enzymolysis to observe and analyze the utilization of egg membrane protein, so as to improve the utilization rate of egg membrane protein.

scholarly journals Ultrafast synthesis of isoquercitrin by enzymatic hydrolysis of rutin in a continuous-flow microreactor

2015 ◽  
Vol 80 (7) ◽  
pp. 853-866 ◽  
Author(s):  
Jun Wang ◽  
Gong An ◽  
Shuangshuang Gu ◽  
Hongsheng Cui ◽  
Xiangyang Wu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Continuous Flow ◽  
Biological Activities ◽  
Batch Reactor ◽  
Maximum Yield ◽  
Enzymatic Reaction ◽  
High Yield ◽  
Flow Microreactor ◽  
Wide Range ◽  
Hydrolysis Of

Isoquercitrin is a rare flavonol glycoside with a wide range of biological activities and is a key synthetic intermediate for the production of enzymatically modified isoquercitrin. In order to establish an ultrafast bioprocess for obtaining isoquercitrin, a novel continuous flow biosynthesis of isoquercitrin using the hesperidinase-catalyzed hydrolysis of rutin in a glass-polydimethylsiloxane (PDMS) microreactor was first carried out. Using the developed microchannel reactor (200?m width, 50?m depth, and 2 m length) with one T-shaped inlet and one outlet, the maximum yield of isoquercitrin (98.6%) was achieved in a short time (40 min) under the following optimum conditions: rutin concentration at 1 g L-1, hesperidinase concentration at 0.1 g mL-1, reaction temperature at 40?C, and a flow rate at 2 ?L min-1. The activation energy value Ea of the enzymatic reaction was 4.61 kJ mol-1, and the reaction rate and volumetric productivity were approximately 16.1-fold and 30% higher, respectively, than those in the batch reactor. Thus, the use of a continuous-flow microreactor for the enzymatic hydrolysis of rutin is an efficient and simple approach to achieve a relative high yield of isoquercitrin.

Preparation of N-Acetyl-D-Glucosamine by Enzymatic Hydrolysis of Amorphous Chitin

2010 ◽  
Vol 160-162 ◽  
pp. 1362-1365 ◽  
Author(s):  
Hong Zhu ◽  
Qiong Hou
Keyword(s):  
Enzymatic Hydrolysis ◽  
High Yield ◽  
Hydrolysis Of ◽  
Amorphous Chitin

Several crude enzymes including cellelase Tv, hemicellulase and lipase, papain as well as cellulase Ac were examined for the effective production of N-acetyl-D-glucosamine (GlcNAc) from amorphous α-chitin. Among the tested crude enzymes, cellulase Tv was found to be the most effective for directly degrading amorphous α-chitin to GlcNAc. High yield of GlcNAc was achieved by choosing the optimum hydrolytic conditions at pH 4.5 and 37°C. By gradually adding, in three tantamount cases, the amount of cellulase Tv to amorphous α-chitin, the yield of GlcNAc reach 55.0%.

Oxidative stress suppression in C. elegans by peptides from dogfish skin via regulation of transcription factors DAF-16 and HSF-1

2022 ◽  
Author(s):  
Xiaoli Ma ◽  
Jiao Li ◽  
Yi Zhang ◽  
Orcun Hacariz ◽  
Jeff Xia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factors ◽  
Enzymatic Hydrolysis ◽  
High Yield ◽  
Regulation Of Transcription ◽  
C Elegans ◽  
Functional Peptides ◽  
Mustelus Canis ◽  
Hydrolysis Of

Functional peptides were obtained via enzymatic hydrolysis of smooth dogfish (Mustelus canis) skin. The enzyme-assisted process was optimized to achieve high yield of smooth dogfish skin peptides (SDSP). Fractions of...

scholarly journals Effect of Pretreatments on the Enzymatic Hydrolysis of High-Yield Bamboo Chemo-Mechanical Pulp by Changing the Surface Lignin Content

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 787
Author(s):  
Lianxin Luo ◽  
Xiaojun Yuan ◽  
Sheng Zhang ◽  
Xuchong Wang ◽  
Mingfu Li ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Enzymatic Hydrolysis ◽  
Laser Scanning ◽  
Lignin Content ◽  
Value Added ◽  
Laser Scanning Microscopy ◽  
High Yield ◽  
Confocal Laser ◽  
Mechanical Pulp ◽  
Hydrolysis Of

Hydrogen peroxide chemo-mechanical pulp (APMP), sulfonated chemo-mechanical pulp (SCMP), and chemical thermomechanical pulp (CTMP) were used as raw materials to explore the effects of hydrogen peroxide (HP), Fenton pretreatment (FP), and ethanol pretreatment (EP) on the enzymatic hydrolysis of high-yield bamboo mechanical pulp (HBMP). The surface lignin distribution and contents of different HBMPs were determined using confocal laser scanning microscopy (CLSM) and X-ray photoelectron spectroscopy (XPS). The correlation between the surface lignin and the enzymatic hydrolysis of HBMP was also investigated. The residue of enzymatic hydrolysis was used to adsorb methylene blue (MB). The results showed that the cracks and fine fibers on the surface of APMP, SCMP, and CTMP increased after FP, when compared to HP and EP. The total removal content of hemicellulose and lignin in SCMP after FP was higher than with HP and EP. Compared to SCMP, the crystallinity increased by 15.4%, and the surface lignin content of Fenton-pretreated SCMP decreased by 11.7%. The enzymatic hydrolysis efficiency of HBMP after FP was higher than with HP and EP. The highest enzymatic hydrolysis of Fenton-pretreated SCMP was 49.5%, which was higher than the enzymatic hydrolysis of Fenton-pretreated APMP and CTMP. The removal rate of MB reached 94.7% after the adsorption of the enzymatic hydrolysis residue of SCMP. This work provides an effective approach for a high value-added utilization of high-yield bamboo pulp.

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