Energy-efficient butanol production by Clostridium acetobutylicum with histidine kinase knockouts to improve strain tolerance and process robustness

2021 ◽  
Author(s):  
Guangqing Du ◽  
Chao Zhu ◽  
Mengmeng Xu ◽  
Lan Wang ◽  
Shang-Tian Yang ◽  
...  
Keyword(s):  
Histidine Kinase ◽  
Energy Efficient ◽  
Clostridium Acetobutylicum ◽  
Abe Fermentation ◽  
Butanol Production ◽  
Process Robustness ◽  
Strain Tolerance ◽  
Acetone Butanol Ethanol

Under stress, Clostridium acetobutylicum sporulates and halts its metabolism, which limits its use in industrial acetone-butanol-ethanol (ABE) fermentation. It is challenging to manipulate the highly regulated sporulation program used by...

scholarly journals Effect of lignocellulose-derived weak acids on butanol production byClostridium acetobutylicumunder different pH adjustment conditions

RSC Advances ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 1967-1975 ◽  
Author(s):  
Jianhui Wang ◽  
Hongyan Yang ◽  
Gaoxaing Qi ◽  
Xuecheng Liu ◽  
Xu Gao ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Formic Acid ◽  
Clostridium Acetobutylicum ◽  
Levulinic Acid ◽  
Abe Fermentation ◽  
Butanol Production ◽  
Weak Acids ◽  
Ph Adjustment ◽  
Acetone Butanol Ethanol

The effects of formic acid, acetic acid and levulinic acid on acetone–butanol–ethanol (ABE) fermentation under different pH adjustment conditions were investigated usingClostridium acetobutylicumas the fermentation strain.

Butanol Production Using Carbohydrate-Enriched Chlorella vulgaris as Feedstock

2013 ◽  
Vol 830 ◽  
pp. 122-125 ◽  
Author(s):  
Yue Wang ◽  
Wan Qian Guo ◽  
Jo Shu Chang ◽  
Nan Qi Ren
Keyword(s):  
Sulfuric Acid ◽  
Chlorella Vulgaris ◽  
Clostridium Acetobutylicum ◽  
Cost Effective ◽  
Abe Fermentation ◽  
Third Generation ◽  
Butanol Production ◽  
Butanol Fermentation ◽  
Acetone Butanol Ethanol ◽  
Pretreated Biomass

Butanol is considered as a potential fuel due to several advantage over ethanol. However, it is still of urgent demand to identify better feedstock, which is more renewable and cost-effective, for the production of bio-butanol. Microalgae can mitigate CO2 emission and convert CO2 into biomass abundant in carbohydrates, and thus appear as emerging third-generation feedstock for fermentation. In this study, an isolated microalga Chlorella vulgaris was cultivated photoautotrophically and the biomass was then harvested for the use in butanol fermentation with a Clostridium acetobutylicum strain via acetone-butanol-ethanol (ABE) fermentation. The results show that 3.37 g L-1 of butanol was produced from 111g of acid-pretreated biomass of C. vulgaris. This demonstrates the potential of using microalgal feedstock for fermentative butanol production. The results also suggest that to improve hydrolysis efficiency of C. vulgaris, higher concentration of sulfuric acid (>2%) should be used.

Integrated ABE fermentation–adsorption process for enhanced butanol production by Clostridium acetobutylicum

2018 ◽  
Vol 44 ◽  
pp. S41
Author(s):  
F. Raganati ◽  
A. Procentese ◽  
G. Olivieri ◽  
M.E. Russo ◽  
P. Salatino ◽  
...  
Keyword(s):  
Clostridium Acetobutylicum ◽  
Adsorption Process ◽  
Abe Fermentation ◽  
Butanol Production

scholarly journals Effective continuous acetone–butanol–ethanol production with full utilization of cassava by immobilized symbiotic TSH06

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhangnan Lin ◽  
Hongjuan Liu ◽  
Jing Wu ◽  
Petra Patakova ◽  
Barbora Branska ◽  
...  
Keyword(s):  
Large Scale ◽  
Immobilized Cells ◽  
Continuous Fermentation ◽  
Abe Fermentation ◽  
Human Beings ◽  
Butanol Production ◽  
System A ◽  
Glucose System ◽  
Acetone Butanol Ethanol ◽  
Full Utilization

Abstract Background Butanol production by fermentation has recently attracted increasingly more attention because of its mild reaction conditions and environmentally friendly properties. However, traditional feedstocks, such as corn, are food supplies for human beings and are expensive and not suitable for butanol production at a large scale. In this study, acetone, butanol, and ethanol (ABE) fermentation with non-pretreated cassava using a symbiotic TSH06 was investigated. Results In batch fermentation, the butanol concentration of 11.6 g/L was obtained with a productivity of 0.16 g/L/h, which was similar to that obtained from glucose system. A full utilization system of cassava was constructed to improve the fermentation performance, cassava flour was used as the substrate and cassava peel residue was used as the immobilization carrier. ABE fermentation with immobilized cells resulted in total ABE and butanol concentrations of 20 g/L and 13.3 g/L, which were 13.6% and 14.7% higher, respectively, than those of free cells. To further improve the solvent productivity, continuous fermentation was conducted with immobilized cells. In single-stage continuous fermentation, the concentrations of total ABE and butanol reached 9.3 g/L and 6.3 g/L with ABE and butanol productivities of 1.86 g/L/h and 1.26 g/L/h, respectively. In addition, both of the high product concentration and high solvent productivity were achieved in a three-stage continuous fermentation. The ABE productivity and concentration was 1.12 g/L/h and 16.8 g/L, respectively. Conclusions The results indicate that TSH06 could produce solvents from cassava effectively. This study shows that ABE fermentation with cassava as a substrate could be an efficient and economical method of butanol production.

scholarly journals Milling byproducts are an economically viable substrate for butanol production using clostridial ABE fermentation

2020 ◽  
Vol 104 (20) ◽  
pp. 8679-8689
Author(s):  
Nils Thieme ◽  
Johanna C. Panitz ◽  
Claudia Held ◽  
Birgit Lewandowski ◽  
Wolfgang H. Schwarz ◽  
...  
Keyword(s):  
Clostridium Beijerinckii ◽  
Abe Fermentation ◽  
Liquid Fuels ◽  
Butanol Production ◽  
Enzymatic Pretreatment ◽  
Profitability Analysis ◽  
Key Points ◽  
Wide Range ◽  
Wheat Middlings ◽  
Acetone Butanol Ethanol

Abstract Butanol is a platform chemical that is utilized in a wide range of industrial products and is considered a suitable replacement or additive to liquid fuels. So far, it is mainly produced through petrochemical routes. Alternative production routes, for example through biorefinery, are under investigation but are currently not at a market competitive level. Possible alternatives, such as acetone-butanol-ethanol (ABE) fermentation by solventogenic clostridia are not market-ready to this day either, because of their low butanol titer and the high costs of feedstocks. Here, we analyzed wheat middlings and wheat red dog, two wheat milling byproducts available in large quantities, as substrates for clostridial ABE fermentation. We could identify ten strains that exhibited good butanol yields on wheat red dog. Two of the best ABE producing strains, Clostridium beijerinckii NCIMB 8052 and Clostridium diolis DSM 15410, were used to optimize a laboratory-scale fermentation process. In addition, enzymatic pretreatment of both milling byproducts significantly enhanced ABE production rates of the strains C. beijerinckii NCIMB 8052 and C. diolis DSM 15410. Finally, a profitability analysis was performed for small- to mid-scale ABE fermentation plants that utilize enzymatically pretreated wheat red dog as substrate. The estimations show that such a plant could be commercially successful. Key points • Wheat milling byproducts are suitable substrates for clostridial ABE fermentation. • Enzymatic pretreatment of wheat red dog and middlings increases ABE yield. • ABE fermentation plants using wheat red dog as substrate are economically viable.

Butanol production from the effluent of hydrogen fermentation

2011 ◽  
Vol 63 (6) ◽  
pp. 1236-1240 ◽  
Author(s):  
W. H. Chen ◽  
S. Y. Chen ◽  
S. J. Chao ◽  
Z. C. Jian
Keyword(s):  
Consumption Rate ◽  
Clostridium Beijerinckii ◽  
Abe Fermentation ◽  
Acetate Buffer ◽  
Microbial Populations ◽  
Butanol Production ◽  
Practical Application ◽  
Carbohydrate Consumption ◽  
Acetone Butanol Ethanol ◽  
Acetate Butyrate

The purpose of the study was to recover butanol from the effluent of the hydrogen-producing bioreactor containing acetate, butyrate, and carbohydrate. The butanol production by Clostridium beijerinckii NRRL B592 was evaluated under both unsterilized and sterilized conditions for examining the potential of butanol production for the practical application. Sucrose of 10 g/L and butyrate of 2 g/L coupled with acetate buffer were used to mimic the effluent. Sucrose was completely consumed in the both unsterilized and sterilized conditions during acetone-butanol-ethanol (ABE) fermentation. However, the results illustrate that the carbohydrate consumption rate in the unsterilized condition was higher than that in the sterilized condition. The maximum butanol concentrations of 3,500 and 3,750 mg/L were achieved in the sterilized and unsterilized conditions, respectively. Meanwhile, it was found that the acetate and the butyrate concentrations of 600 and 1,500 mg/L, and 300 and 1,000 mg/L were ingested to yield butanol in the sterilized condition and in the unsterilized condition, respectively. The results concluded that high levels of acetate and butyrate could eliminate the interference of other microbial populations, resulting in the enrichment of C. beijerinckii NRRL B592 in the fermentor. The butanol production by C. beijerinckii NRRL B592 could be, therefore, produced from the effluent of the hydrogen-producing bioreactor. It promised that the microbial butanol production is one of attractive bioprocesses to recover energy from wastes.

scholarly journals Comparison of acetone–butanol–ethanol fermentation and ethanol catalytic upgrading as pathways for butanol production: A techno-economic and environmental assessment

2021 ◽  
Vol 8 (2) ◽  
pp. 1384-1399
Author(s):  
Estefanny Carmona-Garcia ◽  
Paula Andrea Marín-Valencia ◽  
Juan Camilo Solarte-Toro ◽  
Konstantinos Moustakas ◽  
Carlos Ariel Cardona-Alzate
Keyword(s):  
Environmental Impact ◽  
Fermentation Process ◽  
Value Added ◽  
Abe Fermentation ◽  
Economic Feasibility ◽  
Biofuel Production ◽  
Selling Price ◽  
Butanol Production ◽  
Catalytic Upgrading ◽  
Acetone Butanol Ethanol

Butanol is an important compound used as a building block for producing value-added products and an energy carrier. The main butanol production pathways are conventional acetone–butanol–ethanol (ABE) fermentation and catalytic upgrading of ethanol. On the other hand, the application of biomass as a promising substrate for biofuel production has been widely considered recently. However, few studies have compared different butanol production pathways using biomass as raw material. In light of that, the present work aims (i) to provide a short review of the catalytic ethanol upgrading and (ii) to compare conventional ABE fermentation and catalytic ethanol upgrading processes from the economic and environmental perspectives. Aspen Plus v9.0 was used to simulate both processes. The economic and environmental assessments were carried out considering the Colombian economic context, a gate-to-gate approach, and single impact categories. Considering a processing scale of 1000 ton/d, the conventional ABE fermentation process presented a more favorable technical, economic, and environmental performance for butanol production from biomass. It also offered lower net energy consumption (i.e., 57.9 GJ/ton of butanol) and higher butanol production (i.e., 2.59 ton/h). Nevertheless, the proposed processing scale was insufficient to reach economic feasibility for both processes. To overcome this challenge, the minimum processing scale had to be higher than 1584 ton/d and 1920 ton/d for conventional ABE fermentation and catalytic ethanol upgrading, respectively. Another critical factor in enhancing the economic feasibility of both butanol production pathways was the minimum selling price of butanol. More specifically, prices higher than 1.56 USD/kg and 1.80 USD/kg would be required for conventional ABE fermentation and catalytic ethanol upgrading, respectively. From the environmental impact point of view, the conventional ABE fermentation process led to a lower potential environmental impact than catalytic ethanol upgrading (0.12 PEI/kg vs. 0.18 PEI/kg, respectively).

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