scholarly journals Polymer principles behind solubilizing lignin with organic cosolvents for bioenergy

2020 ◽  
Vol 22 (13) ◽  
pp. 4331-4340 ◽  
Author(s):  
Derya Vural ◽  
Jeremy C. Smith ◽  
Loukas Petridis
Keyword(s):  
Organic Cosolvents ◽  
Polymer Theory

Polymer theory, developed for simple homopolymers, is applicable to the interaction of lignin with aprotic organic pretreatment solvents.

The effect of organic cosolvents on the oxygen affinity of fetal hemoglobin

1991 ◽  
Vol 39 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Valeria Militello ◽  
Eugenio Vitrano ◽  
Antonio Cupane
Keyword(s):  
Oxygen Affinity ◽  
Fetal Hemoglobin ◽  
Organic Cosolvents

Polymer theory: path integrals and scaling

2000 ◽  
Vol 336 (3) ◽  
pp. 167-254 ◽  
Author(s):  
T.A. Vilgis
Keyword(s):  
Path Integrals ◽  
Polymer Theory

scholarly journals Enantioselective Epoxidation by Flavoprotein Monooxygenases Supported by Organic Solvents

Catalysts ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 568 ◽  
Author(s):  
Daniel Eggerichs ◽  
Carolin Mügge ◽  
Julia Mayweg ◽  
Ulf-Peter Apfel ◽  
Dirk Tischler
Keyword(s):  
Organic Solvents ◽  
Nicotinamide Adenine Dinucleotide ◽  
In Silico ◽  
Flavin Adenine Dinucleotide ◽  
Substrate Preference ◽  
Solvent Tolerance ◽  
Organic Cosolvents ◽  
Two Component ◽  
Enantioselective Epoxidation ◽  
Styrene Derivatives

Styrene and indole monooxygenases (SMO and IMO) are two-component flavoprotein monooxygenases composed of a nicotinamide adenine dinucleotide (NADH)-dependent flavin adenine dinucleotide (FAD)-reductase (StyB or IndB) and a monooxygenase (StyA or IndA). The latter uses reduced FAD to activate oxygen and to oxygenate the substrate while releasing water. We circumvented the need for the reductase by direct FAD reduction in solution using the NAD(P)H-mimic 1-benzyl-1,4-dihydronicotinamide (BNAH) to fuel monooxygenases without NADH requirement. Herein, we report on the hitherto unknown solvent tolerance for the indole monooxygenase from Gemmobacter nectariphilus DSM15620 (GnIndA) and the styrene monooxygenase from Gordonia rubripertincta CWB2 (GrStyA). These enzymes were shown to convert bulky and rather hydrophobic styrene derivatives in the presence of organic cosolvents. Subsequently, BNAH-driven biotransformation was furthermore optimized with regard to the applied cosolvent and its concentration as well as FAD and BNAH concentration. We herein demonstrate that GnIndA and GrStyA enable selective epoxidations of allylic double bonds (up to 217 mU mg−1) in the presence of organic solvents such as tetrahydrofuran, acetonitrile, or several alcohols. Notably, GnIndA was found to resist methanol concentrations up to 25 vol.%. Furthermore, a diverse substrate preference was determined for both enzymes, making their distinct use very interesting. In general, our results seem representative for many IMOs as was corroborated by in silico mutagenetic studies.

Effect of organic cosolvents on the free solution mobility of curved and normal DNA molecules

2006 ◽  
Vol 27 (8) ◽  
pp. 1462-1470 ◽  
Author(s):  
Yongjun Lu ◽  
Earle Stellwagen ◽  
Nancy C. Stellwagen
Keyword(s):  
Free Solution ◽  
Dna Molecules ◽  
Organic Cosolvents
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