Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway

2021 ◽  
Author(s):  
Jinhong Xu ◽  
Hui Xiong ◽  
Zhongqiu Zhao ◽  
Miao Luo ◽  
Yankun Ju ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Cardiovascular Diseases ◽  
Allergic Contact Dermatitis ◽  
Therapeutic Effects ◽  
Erk Pathway ◽  
Main Components ◽  
Allergic Contact

Genistein is one of the main components of soybeans, which has been reported potentially to treat obesity, cancer, osteoporosis and cardiovascular diseases. Recently, genistein has been shown therapeutic effects on...

scholarly journals Therapeutic Benefit in Allergic Dermatitis Derived from the Inhibitory Effect of Byakkokaninjinto on the Migration of Plasmacytoid Dendritic Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Takeshi Yamamoto ◽  
Yue Zhang ◽  
Ai Kigasawa ◽  
Shusaku Hayashi ◽  
Makoto Kadowaki
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
Inhibitory Effect ◽  
Plasmacytoid Dendritic Cells ◽  
Lymphoid Tissues ◽  
Therapeutic Effects ◽  
Immunological Diseases ◽  
Allergic Contact

Dendritic cells (DCs) are well known to be essential immunocytes involved in innate and adaptive immunity. DCs are classified as conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs). Recently, the accumulation of pDCs in inflamed tissues and lymphoid tissues has been considered to be a possible contributing factor in the development of immunological diseases, but little is known about the pathophysiological roles of pDCs in immunological diseases. To date, many studies have demonstrated that many kinds of Kampo formulas can regulate immunological reactions in human immune diseases. Thus, we screened Kampo formulas to identify an agent that inhibits pDC migration. Furthermore, we investigated the therapeutic effects of these formulas on a murine DNFB-induced allergic contact dermatitis model. Bone marrow-derived pDCs (BMpDCs) were derived from the bone marrow cells of BALB/c mice in a culture medium with Flt3 ligand. The effects of Kampo formulas on BMpDC migration were evaluated by assessing the number, velocity, and directionality of BMpDCs chemotaxing toward the more concentrated side of a chemokine (C-C motif) ligand 21 (CCL21) gradient. The Kampo formulas that exerted inhibitory effects on pDC migration were orally administered to DNFB-induced allergic contact dermatitis model mice. Byakkokaninjinto reduced the number of migrated BMpDCs and suppressed the velocity and directionality of BMpDC migration in a chemotaxis assay. Gypsum Fibrosum and Ginseng Radix, which are components of byakkokaninjinto, obviously suppressed the velocity of BMpDC migration. Furthermore, Gypsum Fibrosum significantly suppressed the directionality of BMpDC migration. In DNFB-induced allergic contact dermatitis model mice, byakkokaninjinto markedly abrogated ear swelling in late-phase allergic reactions. In conclusions, byakkokaninjinto, which has an inhibitory effect on pDC migration, was able to prevent the occurrence of allergic contact dermatitis, suggesting that pDCs were involved in the onset of allergic contact dermatitis in the mouse model. Therefore, byakkokaninjinto is anticipated to be a therapeutic agent for disorders related to pDC migration.

scholarly journals Topical Apigenin Alleviates Cutaneous Inflammation in Murine Models

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Mao-Qiang Man ◽  
Melanie Hupe ◽  
Richard Sun ◽  
George Man ◽  
Theodora M. Mauro ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Stratum Corneum ◽  
Allergic Contact Dermatitis ◽  
Topical Application ◽  
Therapeutic Effects ◽  
Irritant Contact Dermatitis ◽  
Murine Models ◽  
Cutaneous Inflammation ◽  
Allergic Contact ◽  
Topical Applications

Herbal medicines have been used in preventing and treating skin disorders for centuries. It has been demonstrated that systemic administration of chrysanthemum extract exhibits anti-inflammatory properties. However, whether topical applications of apigenin, a constituent of chrysanthemum extract, influence cutaneous inflammation is still unclear. In the present study, we first tested whether topical applications of apigenin alleviate cutaneous inflammation in murine models of acute dermatitis. The murine models of acute allergic contact dermatitis and acute irritant contact dermatitis were established by topical application of oxazolone and phorbol 12-myristate 13-acetate (TPA), respectively. Inflammation was assessed in both dermatitis models by measuring ear thickness. Additionally, the effect of apigenin on stratum corneum function in a murine subacute allergic contact dermatitis model was assessed with an MPA5 physiology monitor. Our results demonstrate that topical applications of apigenin exhibit therapeutic effects in both acute irritant contact dermatitis and allergic contact dermatitis models. Moreover, in comparison with the vehicle treatment, topical apigenin treatment significantly reduced transepidermal water loss, lowered skin surface pH, and increased stratum corneum hydration in a subacute murine allergic contact dermatitis model. Together, these results suggest that topical application of apigenin could provide an alternative regimen for the treatment of dermatitis.

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