Digestive characteristics and peptide release from wheat embryo proteins in vitro

2021 ◽  
Author(s):  
Wenjing Chen ◽  
Aimei Liao ◽  
Yinchen Hou ◽  
Long Pan ◽  
Guanghai Yu ◽  
...  
Keyword(s):  
Wheat Embryo ◽  
Peptide Release

The nutritional repair function of wheat embryo protein is determined by its digestive fate.

Synthesis and turnover of proteins and mRNA in germinating wheat embryos

1982 ◽  
Vol 60 (3) ◽  
pp. 389-397 ◽  
Author(s):  
Zbyszko F. Grzelczak ◽  
Mark H. Sattolo ◽  
Linda K. Hanley-Bowdoin ◽  
Theresa D. Kennedy ◽  
Byron G. Lane
Keyword(s):  
Growth Phase ◽  
Time Course ◽  
Phase 1 ◽  
Major Product ◽  
Lag Phase ◽  
Wheat Embryo ◽  
Phase Development ◽  
Wheat Embryos

The most prominent methionine-labeled protein made when cell-free systems are programmed with bulk mRNA from dry wheat embryos has been identified with what may be the most abundant protein in dry wheat embryos. The protein has been brought to purity and has a distinctive amino acid composition, Gly and Glx accounting for almost 40% of the total amino acids. Designated E because of its conspicuous association with early imbibition of dry wheat embryos, the protein and its mRNA are abundant during the "early" phase (0–1 h) of postimbibition development, and easily detected during "lag" phase (1–5 h), but they are almost totally degraded soon after entry into the "growth" phase of development, by about 10 h postimbibition.The most prominent methionine-labeled protein peculiar to the cell-free translational capacity of bulk mRNA from "growth" phase embryos is not detected as a product of in vivo synthesis. Its electrophoretic properties and its time course of emergence, after 5 h postimbibition development, suggest that this major product of cell-free synthesis may be an in vitro counterpart to a prominent methionine-labeled protein made only in vivo, by "growth" phase embryos. Designated G because of its conspicuous association with "growth" phase development, the cell-free product does not comigrate with any prominent dye-stained band in electrophoretic distributions of wheat proteins. The suspected cellular counterpart to G, also, does not comigrate with a prominent dye-stained wheat protein during electrophoresis, and although found in particulate as well as soluble fractions of wheat embryo homogenates it is not concentrated in either nuclei or mitochondria, as isolated.

Tapentadol inhibits calcitonin gene-related peptide release from rat brainstem in vitro

Peptides ◽  
2014 ◽  
Vol 56 ◽  
pp. 8-13 ◽  
Author(s):  
Maria Cristina Greco ◽  
Lucia Lisi ◽  
Diego Currò ◽  
Pierluigi Navarra ◽  
Giuseppe Tringali
Keyword(s):  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Peptide Release ◽  
Rat Brainstem

scholarly journals Putative Autocleavage of Outer Capsid Protein μ1, Allowing Release of Myristoylated Peptide μ1N during Particle Uncoating, Is Critical for Cell Entry by Reovirus

2004 ◽  
Vol 78 (16) ◽  
pp. 8732-8745 ◽  
Author(s):  
Amy L. Odegard ◽  
Kartik Chandran ◽  
Xing Zhang ◽  
John S. L. Parker ◽  
Timothy S. Baker ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Structural Changes ◽  
General Mechanism ◽  
Membrane Penetration ◽  
Outer Capsid Protein ◽  
Core Proteins ◽  
Peptide Release ◽  
Outer Capsid

ABSTRACT Several nonenveloped animal viruses possess an autolytic capsid protein that is cleaved as a maturation step during assembly to yield infectious virions. The 76-kDa major outer capsid protein μ1 of mammalian orthoreoviruses (reoviruses) is also thought to be autocatalytically cleaved, yielding the virion-associated fragments μ1N (4 kDa; myristoylated) and μ1C (72 kDa). In this study, we found that μ1 cleavage to yield μ1N and μ1C was not required for outer capsid assembly but contributed greatly to the infectivity of the assembled particles. Recoated particles containing mutant, cleavage-defective μ1 (asparagine → alanine substitution at amino acid 42) were competent for attachment; processing by exogenous proteases; structural changes in the outer capsid, including μ1 conformational change and σ1 release; and transcriptase activation but failed to mediate membrane permeabilization either in vitro (no hemolysis) or in vivo (no coentry of the ribonucleotoxin α-sarcin). In addition, after these particles were allowed to enter cells, the δ region of μ1 continued to colocalize with viral core proteins in punctate structures, indicating that both elements remained bound together in particles and/or trapped within the same subcellular compartments, consistent with a defect in membrane penetration. If membrane penetration activity was supplied in trans by a coinfecting genome-deficient particle, the recoated particles with cleavage-defective μ1 displayed much higher levels of infectivity. These findings led us to propose a new uncoating intermediate, at which particles are trapped in the absence of μ1N/μ1C cleavage. We additionally showed that this cleavage allowed the myristoylated, N-terminal μ1N fragment to be released from reovirus particles during entry-related uncoating, analogous to the myristoylated, N-terminal VP4 fragment of picornavirus capsid proteins. The results thus suggest that hydrophobic peptide release following capsid protein autocleavage is part of a general mechanism of membrane penetration shared by several diverse nonenveloped animal viruses.

scholarly journals Genetics of in vitro organogenesis and precocious germination of wheat embryos

1998 ◽  
Vol 21 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Claudia E. Lange ◽  
Luiz C. Federizzi ◽  
Fernando I.F. Carvalho ◽  
Ana L.C. Dornelles ◽  
Cristine L. Handel
Keyword(s):  
Gene Action ◽  
Precocious Germination ◽  
Genetic Determination ◽  
Wheat Embryo ◽  
In Vitro Organogenesis ◽  
Generation Means ◽  
Generation Means Analysis ◽  
Wheat Embryos ◽  
Segregating Population

The genetic bases of in vitro organogenesis and precocious germination of embryos in immature wheat embryo culture were investigated using six Brazilian genotypes and their F1, F2, BC1F1 and BC2F1 generations in a generation means analysis. Four parents and one set of F1’s were also analyzed in a diallel experiment. The results indicated a complex gene action controlling both traits, with additive, dominant and epistatic effects. High broad sense heritability values were found, indicating genetic determination. Considering the complexity of gene control, genetic gain could be achieved by selecting for the traits in advanced generations of the segregating population. Low correlation values between organogenesis, precocious germination, regeneration and somatic embryogenesis (data shown in a previous report) indicated the possibility of obtaining recombinant genotypes.

In vitro modulation of glucagon-like peptide release by DPP-IV inhibitory polyphenol-polysaccharide conjugates of sprouted quinoa yoghurt

2020 ◽  
Vol 324 ◽  
pp. 126857
Author(s):  
Joy Ujiroghene Obaroakpo ◽  
Lu Liu ◽  
Shuwen Zhang ◽  
Jing Lu ◽  
Liu Liu ◽  
...  
Keyword(s):  
Dpp Iv ◽  
Peptide Release ◽  
Glucagon Like Peptide

scholarly journals Impact of human milk pasteurization on the kinetics of peptide release during in vitro dynamic digestion at the preterm newborn stage

2019 ◽  
Vol 281 ◽  
pp. 294-303 ◽  
Author(s):  
Amélie Deglaire ◽  
Samira De Oliveira ◽  
Julien Jardin ◽  
Valérie Briard-Bion ◽  
Florian Kroell ◽  
...  
Keyword(s):  
Human Milk ◽  
Preterm Newborn ◽  
Peptide Release ◽  
Milk Pasteurization ◽  
Kinetics Of

In vitro BMP-2 peptide release from thiolated chitosan based hydrogel

2016 ◽  
Vol 93 ◽  
pp. 314-321 ◽  
Author(s):  
Xujie Liu ◽  
Bo Yu ◽  
Qianli Huang ◽  
Rui Liu ◽  
Qingling Feng ◽  
...  
Keyword(s):  
Thiolated Chitosan ◽  
Peptide Release

Flupirtine inhibits calcitonin-gene related peptide release from rat brainstem in vitro

2012 ◽  
Vol 506 (2) ◽  
pp. 332-335 ◽  
Author(s):  
Giuseppe Tringali ◽  
Maria Cristina Greco ◽  
Alessandro Capuano ◽  
Giuseppe Guerriero ◽  
Diego Currò ◽  
...  
Keyword(s):  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Peptide Release ◽  
Rat Brainstem

scholarly journals Lysine blockage of milk proteins in infant formula impairs overall protein digestibility and peptide release

2020 ◽  
Vol 11 (1) ◽  
pp. 358-369 ◽  
Author(s):  
Hannah E. Zenker ◽  
Glenn A. A. van Lieshout ◽  
Martine P. van Gool ◽  
Marjolijn C. E. Bragt ◽  
Kasper A. Hettinga
Keyword(s):  
Infant Formula ◽  
Milk Proteins ◽  
Protein Digestibility ◽  
Peptide Release ◽  
Peptide Length

High levels of blocked lysine in infant formula lead to increasing average peptide length after in vitro digestion in infants.

Participation of cytokinins at the early stages of embryoidogenesis in vitro in spring bread wheat embryo calli.

Biomics ◽  
2018 ◽  
Vol 10 (2) ◽  
pp. 141-146
Author(s):  
I.R. Galin ◽  
D.Yu. Zaytsev ◽  
O.A. Seldimirova ◽  
N.N. Kruglova
Keyword(s):  
Bread Wheat ◽  
Spring Bread Wheat ◽  
Wheat Embryo ◽  
Early Stages
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