scholarly journals Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata

2020 ◽  
Vol 11 (8) ◽  
pp. 7104-7114
Author(s):  
Kai Qian ◽  
Ting Tan ◽  
Hui Ouyang ◽  
Shi-Lin Yang ◽  
Wei-Feng Zhu ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Deae Cellulose ◽  
Water Soluble ◽  
Hypoglycemic Activity ◽  
Gel Chromatography ◽  
Pueraria Lobata

A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.

scholarly journals Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2740 ◽  
Author(s):  
Ekaterina Baeva ◽  
Roman Bleha ◽  
Ekaterina Lavrova ◽  
Leonid Sushytskyi ◽  
Jana Čopíková ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Pleurotus Ostreatus ◽  
Cold Water ◽  
Water Soluble ◽  
Hot Water ◽  
Biologically Active ◽  
Alkaline Extract ◽  
A Cell ◽  
Oyster Mushrooms

Oyster mushrooms are an interesting source of biologically active glucans and other polysaccharides. This work is devoted to the isolation and structural characterization of polysaccharides from basidiocarps of the cultivated oyster mushroom, Pleurotus ostreatus. Five polysaccharidic fractions were obtained by subsequent extraction with cold water, hot water and two subsequent extractions with 1 m sodium hydroxide. Branched partially methoxylated mannogalactan and slightly branched (1→6)-β-d-glucan predominated in cold- and hot-water-soluble fractions, respectively. Alternatively, these polysaccharides were obtained by only hot water extraction and subsequent two-stage chromatographic separation. The alkali-soluble parts originating from the first alkali extraction were then fractionated by dissolution in dimethyl sulfoxide (DMSO). The polysaccharide insoluble in DMSO was identified as linear (1→3)-α-d-glucan, while branched (1→3)(1→6)-β-d-glucans were found to be soluble in DMSO. The second alkaline extract contained the mentioned branched β-d-glucan together with some proteins. Finally, the alkali insoluble part was a cell wall complex of chitin and β-d-glucans.

Structural characterization of water-soluble polysaccharide from Arctium lappa and its effects on colitis mice

2019 ◽  
Vol 213 ◽  
pp. 89-99 ◽  
Author(s):  
Yao Wang ◽  
Nianfeng Zhang ◽  
Juan Kan ◽  
Xin Zhang ◽  
Xiaonan Wu ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Water Soluble ◽  
Arctium Lappa ◽  
Soluble Polysaccharide

scholarly journals Detection, purification and characterization of a human cancer-associated galactosyltransferase acceptor

1979 ◽  
Vol 178 (2) ◽  
pp. 279-287 ◽  
Author(s):  
D K Podolsky ◽  
M M Weiser
Keyword(s):  
Molecular Weight ◽  
Human Cancer ◽  
Deae Cellulose ◽  
Structural Data ◽  
Low Molecular Weight ◽  
Gel Chromatography ◽  
Purification And Characterization ◽  
Peptide Moiety ◽  
Cellulose Column

A low-molecular-weight acceptor of galactosyltransferase activity was detected in sera and effusions of patients with extensive maligant disease. This substance was purified to homogeneity from both human serum and effusion by using sequential charcoal/Celite and DEAE-cellulose column chromatography. The purified acceptor was shown to act as substrate for both purified normal and cancer-associated human galactosyltransferase (EC 2.4.1.22) isoenzymes, but had a higher affinity for the cancer-associated isoenzyme (Km = 20 microM) than for the normal isoenzyme (Km = 500 microM). The substrate was found to be a glycopeptide with mol.wt. approx. 3600 determined by polyacrylamide-gel chromatography. Carbohyydate analysis demonstrated only the presence of glucosamine and mannose. Amino acid analysis revealed that the peptide moiety consisted of eight different amino acids, including two residues of asparagine and one residue of serine, but no threonine. These structural data suggest that the acceptor is a fraction of an asparagine-glucosamine type of glycoprotein.

Biodegradation of Lignocellulose by White-Rot Fungi: Structural Characterization of Water-Soluble Hemicelluloses

2013 ◽  
Vol 6 (4) ◽  
pp. 1154-1164 ◽  
Author(s):  
Ling-Ping Xiao ◽  
Zheng-Jun Shi ◽  
Yuan-Yuan Bai ◽  
Wei Wang ◽  
Xue-Ming Zhang ◽  
...  
Keyword(s):  
Structural Characterization ◽  
White Rot Fungi ◽  
Water Soluble ◽  
White Rot

Synthesis, study and structural characterization of a new water soluble hexanuclear silver(I) cluster with the 2-mercapto-nicotinic acid with possible antiviral activity

2003 ◽  
Vol 343 ◽  
pp. 361-365 ◽  
Author(s):  
Panagiotis C Zachariadis ◽  
Sotiris K Hadjikakou ◽  
Nick Hadjiliadis ◽  
Adonis Michaelides ◽  
Stavroula Skoulika ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Nicotinic Acid ◽  
Structural Characterization ◽  
Water Soluble

Structural characterization of water-soluble polysaccharides from Opuntia monacantha cladodes in relation to their anti-glycated activities

2007 ◽  
Vol 105 (4) ◽  
pp. 1480-1486 ◽  
Author(s):  
Mouming Zhao ◽  
Ning Yang ◽  
Bao Yang ◽  
Yueming Jiang ◽  
Guihe Zhang
Keyword(s):  
Structural Characterization ◽  
Water Soluble

NMR spectroscopic structural characterization of a water-soluble β-(1 → 3, 1 → 6)-glucan from Aureobasidium pullulans

2017 ◽  
Vol 174 ◽  
pp. 876-886 ◽  
Author(s):  
Hiroyuki Kono ◽  
Nobuhiro Kondo ◽  
Katsuki Hirabayashi ◽  
Makoto Ogata ◽  
Kazuhide Totani ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Aureobasidium Pullulans ◽  
Water Soluble
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