Deciphering calcium-binding behaviors of casein phosphopeptides by experimental approaches and molecular simulation

2020 ◽  
Vol 11 (6) ◽  
pp. 5284-5292 ◽  
Author(s):  
Minna Luo ◽  
Jie Xiao ◽  
Shengwei Sun ◽  
Fengchao Cui ◽  
Guo Liu ◽  
...  
Keyword(s):  
Molecular Simulation ◽  
Binding Sites ◽  
Calcium Binding ◽  
Experimental Methods ◽  
Binding Modes ◽  
Casein Phosphopeptides ◽  
Experimental Approaches ◽  
Calcium Binding Sites

Deciphering calcium-binding sites of CPPs and three calcium-binding modes with experimental methods and molecular simulation.

scholarly journals Mutation of the high affinity calcium binding sites in cardiac troponin C.

1992 ◽  
Vol 267 (2) ◽  
pp. 825-831 ◽  
Author(s):  
J C Negele ◽  
D G Dotson ◽  
W Liu ◽  
H L Sweeney ◽  
J A Putkey
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Cardiac Troponin ◽  
Troponin C ◽  
High Affinity ◽  
Cardiac Troponin C ◽  
Calcium Binding Sites

Rat GTP cyclohydrolase I is a homodecameric protein complex containing high-affinity calcium-binding sites 1 1Edited by W. Baumeister

1998 ◽  
Vol 279 (1) ◽  
pp. 189-199 ◽  
Author(s):  
Michel O Steinmetz ◽  
Christoph Plüss ◽  
Urs Christen ◽  
Bettina Wolpensinger ◽  
Ariel Lustig ◽  
...  
Keyword(s):  
Protein Complex ◽  
Binding Sites ◽  
Calcium Binding ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase ◽  
High Affinity ◽  
Calcium Binding Sites

scholarly journals Characterization of the calcium-binding sites of calcineurin B

FEBS Letters ◽  
1995 ◽  
Vol 362 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Lazaros T Kakalis ◽  
Michael Kennedy ◽  
Robert Sikkink ◽  
Frank Rusnak ◽  
Ian M Armitage
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Calcineurin B ◽  
Calcium Binding Sites

scholarly journals Conformational epitopes at cadherin calcium-binding sites and p120-catenin phosphorylation regulate cell adhesion

2012 ◽  
Vol 23 (11) ◽  
pp. 2092-2108 ◽  
Author(s):  
Yuliya I. Petrova ◽  
MarthaJoy M. Spano ◽  
Barry M. Gumbiner
Keyword(s):  
Cell Surface ◽  
Conformational Changes ◽  
Binding Sites ◽  
Calcium Binding ◽  
Cell Types ◽  
P120 Catenin ◽  
Physiological Regulation ◽  
Conformational Epitopes ◽  
Calcium Binding Sites ◽  
E Cadherin

We investigated changes in cadherin structure at the cell surface that regulate its adhesive activity. Colo 205 cells are nonadhesive cells with a full but inactive complement of E-cadherin–catenin complexes at the cell surface, but they can be triggered to adhere and form monolayers. We were able to distinguish the inactive and active states of E-cadherin at the cell surface by using a special set of monoclonal antibodies (mAbs). Another set of mAbs binds E-cadherin and strongly activates adhesion. In other epithelial cell types these activating mAbs inhibit growth factor–induced down-regulation of adhesion and epithelial morphogenesis, indicating that these phenomena are also controlled by E-cadherin activity at the cell surface. Both types of mAbs recognize conformational epitopes at different interfaces between extracellular cadherin repeat domains (ECs), especially near calcium-binding sites. Activation also induces p120-catenin dephosphorylation, as well as changes in the cadherin cytoplasmic domain. Moreover, phospho-site mutations indicate that dephosphorylation of specific Ser/Thr residues in the N-terminal domain of p120-catenin mediate adhesion activation. Thus physiological regulation of the adhesive state of E-cadherin involves physical and/or conformational changes in the EC interface regions of the ectodomain at the cell surface that are mediated by catenin-associated changes across the membrane.

Calcium-Binding Sites of Calmodulin and Electron Transfer by Inducible Nitric Oxide Synthase†

Biochemistry ◽  
2005 ◽  
Vol 44 (20) ◽  
pp. 7593-7601 ◽  
Author(s):  
Irena Gribovskaja ◽  
Kaleb C. Brownlow ◽  
Sam J. Dennis ◽  
Andrew J. Rosko ◽  
Michael A. Marletta ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Electron Transfer ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Binding Sites ◽  
Calcium Binding ◽  
Calcium Binding Sites ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Functional Roles of the Non-Catalytic Calcium-Binding Sites in the N-Terminal Domain of Human Peptidylarginine Deiminase 4

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e51660 ◽  
Author(s):  
Yi-Liang Liu ◽  
I-Chen Tsai ◽  
Chia-Wei Chang ◽  
Ya-Fan Liao ◽  
Guang-Yaw Liu ◽  
...  
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Peptidylarginine Deiminase ◽  
Functional Roles ◽  
Peptidylarginine Deiminase 4 ◽  
Terminal Domain ◽  
Calcium Binding Sites
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