Chemical characterization and in vivo antioxidant activity of parsley (Petroselinum crispum) aqueous extract

2020 ◽  
Vol 11 (6) ◽  
pp. 5346-5356
Author(s):  
Neide Mara de Menezes Epifanio ◽  
Lynn Rykiel Iglesias Cavalcanti ◽  
Karine Falcão dos Santos ◽  
Priscila Soares Coutinho Duarte ◽  
Piotr Kachlicki ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Chemical Characterization ◽  
Flavonoid Glycosides ◽  
Petroselinum Crispum

Twenty-nine flavonoid glycosides were identified in the aqueous extract (PC) of Petroselinum crispum var. crispum leaves and apiin, the major compound, was isolated thereof.

scholarly journals Antioxidant activity of aqueous extract of passion fruit (Passiflora edulis) leaves: In vitro and in vivo study

2013 ◽  
Vol 53 (2) ◽  
pp. 882-890 ◽  
Author(s):  
Juliana Kelly da Silva ◽  
Cinthia Baú Betim Cazarin ◽  
Talita C. Colomeu ◽  
Ângela Giovana Batista ◽  
Laura M.M. Meletti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Passion Fruit ◽  
In Vivo Study ◽  
Passiflora Edulis

scholarly journals Antioxidant activity and physico-chemical analysis of Campomanesia rufa (O.Berg) Nied. fruits

2020 ◽  
Vol 44 ◽  
Author(s):  
Letícia Aparecida Ferreira de Abreu ◽  
Renato Paiva ◽  
Judith Georgette Alcalde Mosqueira ◽  
Michele Valquíria dos Reis ◽  
Ana Beatriz Silva Araújo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ph Value ◽  
Scientific Information ◽  
Chemical Characterization ◽  
High Water ◽  
Biological Properties ◽  
Soluble Solids ◽  
High Water Content

ABSTRACT Campomanesia rufa (O. Berg) Nied. is a native Cerrado species that presents great edible potential. However, it is a species “in danger of extinction” as recommended by the International Union for the Conservation of Nature (IUCN). No technical and scientific information about the species exists, thus demonstrating the importance of its research. The present work aimed at the physical and chemical characterization of immature and mature C. rufa fruits. The fruits showed a change in coloration from green (b * = 25.11, h = 122.43) to yellowish-green (b * = 34.26 , h = 115.73), an increase in mass (6.54 g to 10.88 g), diameter (23.76 mm to 28.03 mm) and soluble solids (8.00 to 10.80%). The fruits presented high levels of total (1246.35 mg 100 g-1) and soluble pectin (195.93 mg 100 g-1), high water content (78.86 g 100 g-1), low pH value (3.40), and high citric acid content (1.2%). However, the fruits had low protein (0.81 g 100 g-1), lipid contents, and low caloric values (64.76 kcal 100 g-1). The fruits presented significant values of carotenoids, phenolic compounds (312.47 mg 100 g-1), vitamin C (263.60 mg 100 g-1) as well as good in vitro antioxidant activity (1862.81 µM g-1). The results obtained indicate that C. rufa fruits showed a similar composition to the fruits of other Campomanesia species, and their biological properties should be investigated additionally under in vivo conditions.

scholarly journals Identification of phenolic secondary metabolites from Schotia brachypetala Sond. (Fabaceae) and demonstration of their antioxidant activities in Caenorhabditis elegans

2016 ◽  
Author(s):  
Mansour Sobeh ◽  
Esraa A ElHawary ◽  
Herbenya Peixoto ◽  
Rola M Labib ◽  
Heba Handoussa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Caenorhabditis Elegans ◽  
Nuclear Translocation ◽  
Antioxidant Activities ◽  
In Vitro Assays ◽  
Flavonoid Glycosides ◽  
Alcoholic Extract ◽  
C Elegans

Background: Schotia brachypetala Sond. (Fabaceae) is an endemic tree of Southern Africa whose phytochemistry and pharmacology were slightly studied.The present work aimed at profiling the major phenolics compounds present in the hydro-alcoholic extract from S. brachypetala leaves (SBE) using LC/HRESI/MS/MS and NMR and prove their antioxidant capabilities using novel methods. Methods: In vitro assays; DPPH, TEAC persulfate decolorizing kinetic and FRAP assays, and in vivo assays: Caenorhabditis elegans strains maintenance, Intracellular ROS in C. elegans, Survival assay, GFP expression and Subcellular DAF-16 localization were employed to evaluate the antioxidant activity. Results: More than forty polyphenols ,including flavonoid glycosides, galloylated flavonoid glycosides, isoflavones, dihydrochalcones, procyanidins, anthocyanins, hydroxybenzoic acid derivatives, hydrolysable tannins, and traces of methylated and acetylated flavonoid derivatives were identified. Three compounds were isolated and identified from the genus Schotia for the first time, namely gallic acid, myricetin-3-O-α-L-1C4-rhamnoside and quercetin-3-O-L-1C4-rhamnoside.The tested extract was able to protect the worms against juglone induced oxidative stress and attenuate the reactive oxygen species (ROS) accumulation. SBE was also able to attenuate the levels of heat shock protein (HSP) expression. Discussion: A pronounced antioxidant activity in vivo, which can be attributed to its ability to promote the nuclear translocation of DAF-16/FOXO, the main transcription factor regulating the expression of stress response genes. The remarkable antioxidant activity in vitro and in vivo correlates to SBE rich phenolic profile.

scholarly journals Chemical Characterization of Artemisia Annua L. Extract Assessment of Antioxidant Activity in vitro and in vivo Toxicity Studies

2019 ◽  
Vol 70 (6) ◽  
pp. 1903-1907
Author(s):  
Roxana Liana Stan ◽  
Bogdan Sevastre ◽  
Adriana Corina Hangan ◽  
Sanda Bota ◽  
Daniela Hanganu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Artemisia Annua ◽  
Histopathological Examination ◽  
Haematological Toxicity ◽  
Chemical Characterization ◽  
In Vivo Toxicity ◽  
Artemisia Annua L ◽  
Toxicity Studies

The purpose of the study was to demonstrate in vitro antioxidant activity and in vivo toxicity of the Artemisia Annua L. extract. The plant was harvested from Bihor area (Crisul Repede and Negru river valleys), Romania. Preparation of the plant product and of the lyophilizated extract was carried out in accordance with the Romanian Pharmacopeia Xth Edition. Lyophilized extract was evaluated in terms of polyphenol content using HPLC method. Antioxidant activity was highlighted using the DPPH, ABTS and FRAP methods. Hepatic, renal and haematological toxicity studies have been performed on laboratory mice. For this purpose blood and organs were collected. Biochemical and haematological parameters were determined on the blood samples and histopathological examination was performed on organs. In vitro antioxidant effect of Artemisia Annua L extract and its lack of in vivo toxicity were demonstrated. It is desirable to obtain a new phytoproduct harvested from spontaneous flora of Romania with antioxidant / antitumoral properties and which is devoid of toxicity.

scholarly journals Bioactivity, nutritional property, and rapid chemical characterization of aqueous extract of Annona muricata leaf from Mexico

2021 ◽  
Vol 18 (3) ◽  
pp. 611-617
Author(s):  
Ana V. Coria-Téllez ◽  
Eva N. Obledo-Vázquez ◽  
Eduardo Padilla-Camberos ◽  
Marisela González-Ávila ◽  
Moisés Martínez-Velázquez
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Chemical Characterization ◽  
Direct Analysis ◽  
Dependent Manner ◽  
Annona Muricata ◽  
Concentration Dependent Manner ◽  
Anti Cancer

Purpose: To investigate the bioactive and nutritional properties, as well as rapid chemical characterization of aqueous extract of Annona muricata leaf from Mexico Methods: The crude aqueous extract of A. muricata leaf was obtained by decoction. Cytotoxicity was tested against cervicouterine cancer cells (HeLa) using methyl thiazol tetrazolium (MTT) assay. Antioxidant activity was evaluated using 2, 2-diphenylpicrylhydrazyl (DPPH) assay. Nutritional evaluation was carried out according to Association of Official Analytical Chemists (AOAC) procedures. Rapid qualitative chemical characterization of the extract was carried out by direct analysis in real-time mass spectrometry (DART-MS) method. Results: The aqueous extract of A. muricata leaf showed cytotoxicity against HeLa cells and also antioxidant activity in a concentration-dependent manner. Nutritional analysis revealed the presence of carbohydrates, vitamin C, Na, and Fe in the aqueous extract. DART-MS spectra showed the presence of alkaloids and phenols as the major components. Conclusion: The cytotoxic and antioxidant activities of the aqueous extract of A. muricata leaf lend some support for its traditional uses as anti-cancer remedy. These activities are probably due to its active secondary metabolites. Thus, the aqueous extract is a source of healthy nutritional components as well as a potential anti-cancer agent for humans.

scholarly journals Study of Antioxidant Activity of Stalk and Fruit of Solanum melongena L. (Solanaceae)

2019 ◽  
pp. 1-7
Author(s):  
Kady Diatta ◽  
William Diatta ◽  
Alioune Dior Fall ◽  
Serigne Ibra Mbacké Dieng ◽  
Amadou Ibrahima Mbaye ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Solanum Melongena ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
In Vivo Studies ◽  
Ethanol Extracts

Background : Nowadays with the appearance of diseases such as cancer, atherosclerosis, free radicals are often singled out. What motivates scientific research in natural antioxidants. Aim/Objective : The aim of this study was to determine the antioxidant activity of the stalks and the fruit of Solanum melongena L. Study Duration : The period of the study was done on 25th July, 2015 at the Department of Pharmacy, Faculty of Medecine, Pharmacy and Odontology, University of Dakar, Senegal. Methodology : Antioxidant activity was evaluated through two methods (DPPH and FRAP). Results : For the FRAP test, at the highest concentration (83.3 µg/ml) the aqueous extract of the fruit (0.90±0.08) has a higher reducing power compared to those of ethanol extracts from the fruit (0.77±0.41) and the stalk (0.85±0.004). These results remain inferior to that of tannic acid (0.95± 0.0005). The DPPH test reveals that the ethanolic extract of the fruit is more effective in reducing the free radical DPPH with an inhibitory concentration 50 (IC 50) equal to 3.37±0.03 μg / ml, followed by the ethanolic extract of the stalks (IC 50 = 4.46±0.24 μg / ml) and finally the aqueous extract of the fruit (IC50 = 9.6±0.026 μg / ml). Conclusion : These results make it possible to confirm the in vitro activity of the parts studied, but in vivo studies are necessary in order to know the acute and chronic toxicities. Finally, perform a bio-guided fractionation to determine the molecules responsible for the antioxidant activity.

scholarly journals Effect of Salmonella typhimurium infection on rat's cell oxidation and in vivo antioxidant activity of Vitellaria paradoxa and Ludwigia abyssinica aqueous extract

2015 ◽  
Vol 5 (1) ◽  
pp. 38-46 ◽  
Author(s):  
Siméon Pierre Chegaing Fodouop ◽  
Donatien Gatsing ◽  
Benjamin Talom Tangue ◽  
Richard Simo Tagne ◽  
Sédric Donald Tala ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Salmonella Typhimurium ◽  
Aqueous Extract ◽  
Vitellaria Paradoxa

In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit

2008 ◽  
Vol 106 (3) ◽  
pp. 888-895 ◽  
Author(s):  
Hua Wang ◽  
Xiang Dong Gao ◽  
Gao Chao Zhou ◽  
Lei Cai ◽  
Wen Bing Yao
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract
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