Microbial response of nitrifying biofilms to cold-shock

2020 ◽  
Vol 6 (12) ◽  
pp. 3428-3439
Author(s):  
Warsama Ahmed ◽  
Robert Delatolla
Keyword(s):  
Cold Shock ◽  
Microbial Response ◽  
Nitrification Kinetics

The cold-shocked system, rapidly cooled from 10 °C to 1 °C, was characterized by lower nitrification kinetics and differences in biomass and microbiome responses compared to an acclimatized system operated in parallel and allowed to acclimatize.

Technical solutions of vibration safety cold riveting performed ship repair

2020 ◽  
pp. 172-179
Author(s):  
A.Ya. Rozinov
Keyword(s):  
Aluminum Alloy ◽  
Cold Shock ◽  
Vibration Disease ◽  
Shock Absorbers ◽  
Heating And Cooling ◽  
The Press ◽  
Press Equipment ◽  
Technical Solutions ◽  
Ship Repair ◽  
Reverse Methods

The parameters of heating and cooling of rivets made of steel and aluminum alloy, as well as data of fi lling holes with these rivets during hot and cold riveting are compared. The features of the process of performing cold impact riveting by direct and reverse methods, as well as the possibility of reducing the force of this riveting by improving the closing heads and constructive transformation of the rivets themselves are considered. Features of physiological infl uence of cold shock riveting on hands, elbows and shoulders of workers are determined. Describes the construction of riveting hammers and supports with spring shock absorbers that prevent the disease of workers vibration disease, portable riveting presses of pneumatic and hydraulic action. A description of the press equipment and technology of bolt-riveting connections, allowing mechanizing the process of cold riveting is given.

Study on the transgenic tobacco expressing truncated wheat cold shock protein gene TA3-13 and analysis of disease resistance

2011 ◽  
Vol 33 (5) ◽  
pp. 520-526 ◽  
Author(s):  
Na LI ◽  
Xiu-Zhen DU ◽  
Xiao-Mei PAN ◽  
Jin-Sheng WANG ◽  
Cong-Feng SONG
Keyword(s):  
Disease Resistance ◽  
Transgenic Tobacco ◽  
Cold Shock ◽  
Protein Gene ◽  
Cold Shock Protein

scholarly journals Listeria monocytogenes Cold Shock Proteins: Small Proteins with A Huge Impact

2021 ◽  
Vol 9 (5) ◽  
pp. 1061
Author(s):  
Francis Muchaamba ◽  
Roger Stephan ◽  
Taurai Tasara
Keyword(s):  
Listeria Monocytogenes ◽  
Stress Tolerance ◽  
Cold Shock ◽  
Current Data ◽  
Health Concern ◽  
Stress Exposure ◽  
Cold Shock Proteins ◽  
Cell Functions ◽  
Small Proteins ◽  
Shock Proteins

Listeria monocytogenes has evolved an extensive array of mechanisms for coping with stress and adapting to changing environmental conditions, ensuring its virulence phenotype expression. For this reason, L. monocytogenes has been identified as a significant food safety and public health concern. Among these adaptation systems are cold shock proteins (Csps), which facilitate rapid response to stress exposure. L. monocytogenes has three highly conserved csp genes, namely, cspA, cspB, and cspD. Using a series of csp deletion mutants, it has been shown that L. monocytogenes Csps are important for biofilm formation, motility, cold, osmotic, desiccation, and oxidative stress tolerance. Moreover, they are involved in overall virulence by impacting the expression of virulence-associated phenotypes, such as hemolysis and cell invasion. It is postulated that during stress exposure, Csps function to counteract harmful effects of stress, thereby preserving cell functions, such as DNA replication, transcription and translation, ensuring survival and growth of the cell. Interestingly, it seems that Csps might suppress tolerance to some stresses as their removal resulted in increased tolerance to stresses, such as desiccation for some strains. Differences in csp roles among strains from different genetic backgrounds are apparent for desiccation tolerance and biofilm production. Additionally, hierarchical trends for the different Csps and functional redundancies were observed on their influences on stress tolerance and virulence. Overall current data suggest that Csps have a wider role in bacteria physiology than previously assumed.

scholarly journals Genome-wide CRISPR/Cas9 screening identifies CARHSP1 responsible for radiation resistance in glioblastoma

2021 ◽  
Vol 12 (8) ◽  
Author(s):  
Guo-dong Zhu ◽  
Jing Yu ◽  
Zheng-yu Sun ◽  
Yan Chen ◽  
Hong-mei Zheng ◽  
...  
Keyword(s):  
Cold Shock ◽  
Mrna Level ◽  
Critical Role ◽  
Inflammatory Signaling ◽  
Heat Stable ◽  
Genome Wide ◽  
Attractive Therapeutic Target ◽  
Heat Stable Protein ◽  
Signaling Activation ◽  
Shock Proteins

AbstractGlioblastomas (GBM) is the most common primary malignant brain tumor, and radiotherapy plays a critical role in its therapeutic management. Unfortunately, the development of radioresistance is universal. Here, we identified calcium-regulated heat-stable protein 1 (CARHSP1) as a critical driver for radioresistance utilizing genome-wide CRISPR activation screening. This is a protein with a cold-shock domain (CSD)-containing that is highly similar to cold-shock proteins. CARHSP1 mRNA level was upregulated in irradiation-resistant GBM cells and knockdown of CARHSP1 sensitized GBM cells to radiotherapy. The high expression of CARHSP1 upon radiation might mediate radioresistance by activating the inflammatory signaling pathway. More importantly, patients with high levels of CARHSP1 had poorer survival when treated with radiotherapy. Collectively, our findings suggested that targeting the CARHSP1/TNF-α inflammatory signaling activation induced by radiotherapy might directly affect radioresistance and present an attractive therapeutic target for GBM, particularly for patients with high levels of CARHSP1.

scholarly journals Invertebrate and Microbial Response to Hyporheic Restoration of an Urban Stream

Water ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 481
Author(s):  
Sarah A. Morley ◽  
Linda D. Rhodes ◽  
Anne E. Baxter ◽  
Giles W. Goetz ◽  
Abigail H. Wells ◽  
...  
Keyword(s):  
Hyporheic Zone ◽  
Habitat Diversity ◽  
Urban Stream ◽  
Floodplain Restoration ◽  
Design Elements ◽  
Biological Communities ◽  
Taxa Richness ◽  
Restoration Technique ◽  
Microbial Response ◽  
Face Complex

All cities face complex challenges managing urban stormwater while also protecting urban water bodies. Green stormwater infrastructure and process-based restoration offer alternative strategies that prioritize watershed connectivity. We report on a new urban floodplain restoration technique being tested in the City of Seattle, USA: an engineered hyporheic zone. The hyporheic zone has long been an overlooked component in floodplain restoration. Yet this subsurface area offers enormous potential for stormwater amelioration and is a critical component of healthy streams. From 2014 to 2017, we measured hyporheic temperature, nutrients, and microbial and invertebrate communities at three paired stream reaches with and without hyporheic restoration. At two of the three pairs, water temperature was significantly lower at the restored reach, while dissolved organic carbon and microbial metabolism were higher. Hyporheic invertebrate density and taxa richness were significantly higher across all three restored reaches. These are some of the first quantified responses of hyporheic biological communities to restoration. Our results complement earlier reports of enhanced hydrologic and chemical functioning of the engineered hyporheic zone. Together, this research demonstrates that incorporation of hyporheic design elements in floodplain restoration can enhance temperature moderation, habitat diversity, contaminant filtration, and the biological health of urban streams.

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