scholarly journals Applications of bioluminescence in biotechnology and beyond

2021 ◽  
Author(s):  
Aisha J. Syed ◽  
James C. Anderson
Keyword(s):  
Protein Engineering ◽  
Synthetic Chemistry ◽  
Future Directions

Bioluminescent probes have hugely benefited from the input of synthetic chemistry and protein engineering. Here we review the latest applications of these probes in biotechnology and beyond, with an eye on current limitations and future directions.

scholarly journals The future directions of synthetic chemistry

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Qing Zhu ◽  
Chao Liu
Keyword(s):  
Sustainable Energy ◽  
Synthetic Chemistry ◽  
New Materials ◽  
Future Directions ◽  
The Future

Abstract After being developed over hundred years, synthetic chemistry has created numerous new molecules and new materials to support a better life welfare. Even so, many challenges still remain in synthetic chemistry, higher selectivity, higher efficiency, environmental benign and sustainable energy are never been so wistful before. Herein, several topics surrounded the ability improvement of synthesis and the application enhancement of synthesis will be briefly discussed.

Choice of biocatalyst form for scalable processes

2006 ◽  
Vol 34 (2) ◽  
pp. 301-303 ◽  
Author(s):  
J.M. Woodley
Keyword(s):  
Protein Engineering ◽  
Process Engineering ◽  
Synthetic Chemistry ◽  
Whole Cell ◽  
Recent Developments

The design of biocatalytic processes for industrial synthetic chemistry is determined in large part by the choice of isolated enzyme or whole-cell catalyst form. In the present paper, the considerations for choice are identified and some important classes of bioconversion are discussed in relation to the choice to be made. Recent developments in cell and protein engineering as well as reactor and process engineering are discussed in addition.

scholarly journals Replacing the SpCas9 HNH domain by deaminases generates compact base editors with an alternative targeting scope

2020 ◽  
Author(s):  
Lukas Villiger ◽  
Lukas Schmidheini ◽  
Nicolas Mathis ◽  
Tanja Rothgangl ◽  
Kim Marquart ◽  
...  
Keyword(s):  
Protein Engineering ◽  
Adenosine Deaminase ◽  
Fusion Proteins ◽  
Future Directions ◽  
Site Specific ◽  
Nuclease Domain ◽  
Protospacer Adjacent Motif ◽  
Cas9 Protein ◽  
Adenine Base ◽  
Specific Nucleotide

ABSTRACTBase editors are RNA-guided deaminases that enable site-specific nucleotide transitions. The targeting scope of these Cas-deaminase fusion proteins critically depends on the availability of a protospacer adjacent motif (PAM) at the target locus and is limited to a window within the CRISPR-Cas R-loop, where single stranded (ss)DNA is accessible to the deaminase. Here, we reason that the Cas9-HNH nuclease domain sterically constrains ssDNA accessibility, and demonstrate that omission of this domain expands the editing window. By exchanging the HNH nuclease domain with an adenosine deaminase we furthermore engineer adenine base editor variants (HNHx-ABE) with PAM-proximally shifted editing windows. This work expands the targeting scope of base editors, and provides base editor variants that are substantially smaller. It moreover informs of potential future directions in Cas9 protein engineering, where the HNH domain could be replaced by other enzymes that act on ssDNA.

scholarly journals Correction: The multifaceted nature of antimicrobial peptides: current synthetic chemistry approaches and future directions

2022 ◽  
Author(s):  
Bee Ha Gan ◽  
Josephine Gaynord ◽  
Sam M. Rowe ◽  
Tomas Deingruber ◽  
David R. Spring
Keyword(s):  
Antimicrobial Peptides ◽  
Synthetic Chemistry ◽  
Future Directions

Correction for ‘The multifaceted nature of antimicrobial peptides: current synthetic chemistry approaches and future directions’ by Bee Ha Gan et al., Chem. Soc. Rev., 2021, 50, 7820–7880, DOI: 10.1039/D0CS00729C.

scholarly journals The multifaceted nature of antimicrobial peptides: current synthetic chemistry approaches and future directions

2021 ◽  
Author(s):  
Bee Ha Gan ◽  
Josephine Gaynord ◽  
Sam M. Rowe ◽  
Tomas Deingruber ◽  
David R. Spring
Keyword(s):  
Antimicrobial Peptides ◽  
Physicochemical Properties ◽  
Synthetic Chemistry ◽  
Future Directions

This review discusses the diversity of structure and physicochemical properties of antimicrobial peptides and their derivatives, various chemical synthetic strategies that have been applied in their development, and how this links to their activity.

On Future Directions in Pathology

1982 ◽  
Vol 40 ◽  
pp. 274-277
Author(s):  
Benjamin F. Trump ◽  
Irene K. Berezesky ◽  
Raymond T. Jones
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Clinical Pathology ◽  
Future Directions ◽  
Diagnostic Pathology ◽  
The Past ◽  
Transmission Electron ◽  
Organ Systems ◽  
The Many

The role of electron microscopy and associated techniques is assured in diagnostic pathology. At the present time, most of the progress has been made on tissues examined by transmission electron microscopy (TEM) and correlated with light microscopy (LM) and by cytochemistry using both plastic and paraffin-embedded materials. As mentioned elsewhere in this symposium, this has revolutionized many fields of pathology including diagnostic, anatomic and clinical pathology. It began with the kidney; however, it has now been extended to most other organ systems and to tumor diagnosis in general. The results of the past few years tend to indicate the future directions and needs of this expanding field. Now, in addition to routine EM, pathologists have access to the many newly developed methods and instruments mentioned below which should aid considerably not only in diagnostic pathology but in investigative pathology as well.

Insights into the biochemical and functional characterization of sortase E transpeptidase of Corynebacterium glutamicum

2019 ◽  
Vol 476 (24) ◽  
pp. 3835-3847 ◽  
Author(s):  
Aliyath Susmitha ◽  
Kesavan Madhavan Nampoothiri ◽  
Harsha Bajaj
Keyword(s):  
Protein Engineering ◽  
Cell Attachment ◽  
Functional Characterization ◽  
Protein Structures ◽  
Structural Similarity ◽  
Surface Proteins ◽  
Sortase A ◽  
Peptide Cleavage ◽  
New Findings

Most Gram-positive bacteria contain a membrane-bound transpeptidase known as sortase which covalently incorporates the surface proteins on to the cell wall. The sortase-displayed protein structures are involved in cell attachment, nutrient uptake and aerial hyphae formation. Among the six classes of sortase (A–F), sortase A of S. aureus is the well-characterized housekeeping enzyme considered as an ideal drug target and a valuable biochemical reagent for protein engineering. Similar to SrtA, class E sortase in GC rich bacteria plays a housekeeping role which is not studied extensively. However, C. glutamicum ATCC 13032, an industrially important organism known for amino acid production, carries a single putative sortase (NCgl2838) gene but neither in vitro peptide cleavage activity nor biochemical characterizations have been investigated. Here, we identified that the gene is having a sortase activity and analyzed its structural similarity with Cd-SrtF. The purified enzyme showed a greater affinity toward LAXTG substrate with a calculated KM of 12 ± 1 µM, one of the highest affinities reported for this class of enzyme. Moreover, site-directed mutation studies were carried to ascertain the structure functional relationship of Cg-SrtE and all these are new findings which will enable us to perceive exciting protein engineering applications with this class of enzyme from a non-pathogenic microbe.

Meeting the Best Practice for Hearing Aid Verification in Children: Challenges and Future Directions

2019 ◽  
Vol 28 (4) ◽  
pp. 877-894
Author(s):  
Nur Azyani Amri ◽  
Tian Kar Quar ◽  
Foong Yen Chong
Keyword(s):  
Clinical Practice ◽  
Hearing Aids ◽  
Best Practice ◽  
Hearing Aid ◽  
Evidence Based ◽  
Future Directions ◽  
Major Barrier ◽  
Klang Valley ◽  
Age Range ◽  
Fitting Hearing Aids

Purpose This study examined the current pediatric amplification practice with an emphasis on hearing aid verification using probe microphone measurement (PMM), among audiologists in Klang Valley, Malaysia. Frequency of practice, access to PMM system, practiced protocols, barriers, and perception toward the benefits of PMM were identified through a survey. Method A questionnaire was distributed to and filled in by the audiologists who provided pediatric amplification service in Klang Valley, Malaysia. One hundred eight ( N = 108) audiologists, composed of 90.3% women and 9.7% men (age range: 23–48 years), participated in the survey. Results PMM was not a clinical routine practiced by a majority of the audiologists, despite its recognition as the best clinical practice that should be incorporated into protocols for fitting hearing aids in children. Variations in practice existed warranting further steps to improve the current practice for children with hearing impairment. The lack of access to PMM equipment was 1 major barrier for the audiologists to practice real-ear verification. Practitioners' characteristics such as time constraints, low confidence, and knowledge levels were also identified as barriers that impede the uptake of the evidence-based practice. Conclusions The implementation of PMM in clinical practice remains a challenge to the audiology profession. A knowledge-transfer approach that takes into consideration the barriers and involves effective collaboration or engagement between the knowledge providers and potential stakeholders is required to promote the clinical application of evidence-based best practice.

Translating Principles of Speech Science to Clinical Practice: Current and Future Trends in Craniofacial Disorders

2008 ◽  
Vol 18 (1) ◽  
pp. 31-40 ◽  
Author(s):  
David J. Zajac
Keyword(s):  
Clinical Practice ◽  
Speech Intelligibility ◽  
Current Practice ◽  
Computer Assisted ◽  
Behavioral Management ◽  
Single Word ◽  
Future Directions ◽  
Speech Science ◽  
The Impact ◽  
Opinion Article

Abstract The purpose of this opinion article is to review the impact of the principles and technology of speech science on clinical practice in the area of craniofacial disorders. Current practice relative to (a) speech aerodynamic assessment, (b) computer-assisted single-word speech intelligibility testing, and (c) behavioral management of hypernasal resonance are reviewed. Future directions and/or refinement of each area are also identified. It is suggested that both challenging and rewarding times are in store for clinical researchers in craniofacial disorders.

Sign in / Sign up

Export Citation Format

Share Document