Reactive intermediates for interactome mapping

2021 ◽  
Author(s):  
Ciaran P. Seath ◽  
Aaron D. Trowbridge ◽  
Tom W. Muir ◽  
David W. C. MacMillan
Keyword(s):  
Reactive Intermediates ◽  
Live Cells ◽  
Biomolecular Interactions ◽  
Interactome Mapping

This tutorial review describes enabling methods for determining biomolecular interactions in live cells through the use of in situ generated reactive intermediates.

Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots

2021 ◽  
Vol 1157 ◽  
pp. 338394
Author(s):  
Xiao-Yue Tang ◽  
Yi-Ming Liu ◽  
Xiao-Lin Bai ◽  
Hao Yuan ◽  
Yi-Kao Hu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Carbon Dots ◽  
Live Cells ◽  
Turn On ◽  
Dopamine Detection ◽  
In Situ Formation

In Situ Localization of Enzyme Activity in Live Cells by a Molecular Probe Releasing a Precipitating Fluorochrome

2017 ◽  
Vol 129 (39) ◽  
pp. 11950-11954 ◽  
Author(s):  
Hong-Wen Liu ◽  
Ke Li ◽  
Xiao-Xiao Hu ◽  
Longmin Zhu ◽  
Qiming Rong ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Molecular Probe ◽  
Live Cells

scholarly journals BCAbox Algorithm Expands Capabilities of Raman Microscope for Single Organelles Assessment

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 106 ◽  
Author(s):  
Andrey N. Kuzmin ◽  
Artem Pliss ◽  
Alex Rzhevskii ◽  
Adrian Lita ◽  
Mioara Larion
Keyword(s):  
Raman Spectroscopy ◽  
Raman Spectra ◽  
Live Cells ◽  
Spectroscopy Instrumentation ◽  
Precise Analysis ◽  
Express Analysis ◽  
Immediate Analysis ◽  
Software Toolbox ◽  
Cellular Organelles

Raman microspectroscopy is a rapidly developing technique, which has an unparalleled potential for in situ proteomics, lipidomics, and metabolomics, due to its remarkable capability to analyze the molecular composition of live cells and single cellular organelles. However, the scope of Raman spectroscopy for bio-applications is limited by a lack of software tools for express-analysis of biomolecular composition based on Raman spectra. In this study, we have developed the first software toolbox for immediate analysis of intracellular Raman spectra using a powerful biomolecular component analysis (BCA) algorithm. Our software could be easily integrated with commercial Raman spectroscopy instrumentation, and serve for precise analysis of molecular content in major cellular organelles, including nucleoli, endoplasmic reticulum, Golgi apparatus, and mitochondria of either live or fixed cells. The proposed software may be applied in broad directions of cell science, and serve for further advancement and standardization of Raman spectroscopy.

scholarly journals Base-Mediated Generation of Ketenimines from Ynamides: [3+2] Annulation with Azaallyl Anions.

2020 ◽  
Author(s):  
Agathe C. A. D’Hollander ◽  
Eugénie Romero ◽  
Kamsana Vijayakumar ◽  
Camille le Houérou ◽  
Pascal Retailleau ◽  
...  
Keyword(s):  
Dft Calculation ◽  
Reactive Intermediates ◽  
Reaction Conditions ◽  
Stereo Selectivity

Under basic conditions and heat, ynamides can serve as precursor to ketenimines, whose synthetic potential is often hampered by their difficulty of acces. Herein, we report that they can undergo a [3+2] cycloaddition with 2-azaallyl anions, obtained from benzylimines under the same reaction conditions. This reaction between two highly reactive intermediates, both generated in situ from bench stable starting materials, gives access to various nitrogen-rich heterocycles. The reaction usually proceeds with excellent diastereoselectivity, in favor of the cis adduct. Deuteration experiments and DFT calculation helped rationalize the regio and stereo-selectivity of the process as well as the formation of side-products.<br>

scholarly journals Poly(catecholamine) Coating and Biofunctionalization of CsPbBr3 Microcrystals

2021 ◽  
Author(s):  
Sangyeon Cho ◽  
Seok-Hyun Yun
Keyword(s):  
Lipid Bilayers ◽  
Environmental Stability ◽  
Live Cells ◽  
Functional Coatings ◽  
Common Strategy ◽  
Novel Method ◽  
Halide Perovskites ◽  
The Stability ◽  
New Applications

<p>Lead halide perovskites (LHP) microcrystals are promising materials for various optoelectronic applications. Surface coating on particles is a common strategy to improve their functionality and environmental stability, but LHP is not amenable to most coating chemistries because of its intrinsic weakness against polar solvents. Here, we describe a novel method of synthesizing LHP microcrystals in a super-saturated polar solvent using sonochemistry and applying various functional coatings on individual microcrystals <i>in situ</i>. We synthesize cesium lead bromine perovskite (CsPbBr<sub>3</sub>) microparticles capped with organic poly-norepinephrine (pNE) layers. The catechol group of pNE coordinates to bromine-deficient lead atoms, forming a defect-passivating and diffusion-blocking shell. The pNE layer enhances the stability of CsPbBr<sub>3</sub> in water by 2,000-folds, enabling bright luminescence and lasing from single microcrystals in water. Furthermore, the pNE shell permits biofunctionalization with proteins, small molecules, and lipid bilayers. Luminescence from CsPbBr<sub>3</sub> microcrystals is sustained in water over 1 hour and observed in live cells. The functionalization method may enable new applications of LHP particles in water-rich environments.<b></b></p>

An organic molecular compound for in-situ identification of mitochondrial G-quadruplexes in live cells

2022 ◽  
Author(s):  
Xiaomeng Guo ◽  
Hongbo Chen ◽  
Yan Liu ◽  
Dawei Yang ◽  
Qian Li ◽  
...  
Keyword(s):  
Mitochondrial Gene ◽  
Critical Role ◽  
Diagnosis And Treatment ◽  
Live Cells ◽  
Molecular Compound ◽  
Gene Replication ◽  
Promising Target ◽  
G Quadruplex

Emerging studies have shown that mitochondrial G-quadruplex plays a critical role in regulating mitochondrial gene replication and transcription, which makes it a promising target for the diagnosis and treatment of...

Smart Tissue Culture: in Situ Monitoring of the Activity of Protease Enzymes Secreted from Live Cells Using Nanostructured Photonic Crystals

Nano Letters ◽  
2009 ◽  
Vol 9 (5) ◽  
pp. 2021-2025 ◽  
Author(s):  
Kristopher A. Kilian ◽  
Leo M. H. Lai ◽  
Astrid Magenau ◽  
Siân Cartland ◽  
Till Böcking ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Photonic Crystals ◽  
In Situ Monitoring ◽  
Live Cells

scholarly journals Binding Reaction Sites to Polysiloxanes: Unique Fluorescent Probe for Reversible Detection of ClO–/GSH Pair and the in Situ Imaging in Live Cells and Zebrafish

2019 ◽  
Vol 91 (3) ◽  
pp. 1719-1723 ◽  
Author(s):  
Yujing Zuo ◽  
Yu Zhang ◽  
Baoli Dong ◽  
Zhiming Gou ◽  
Tingxin Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Live Cells ◽  
Binding Reaction ◽  
In Situ Imaging
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