Influence of a single ether bond on assembly, orientation, and miscibility of phosphocholine lipids at the air–water interface

2021 ◽  
Author(s):  
Matthias Hoffmann ◽  
Simon Drescher ◽  
Christian Schwieger ◽  
Dariush Hinderberger
Keyword(s):  
Supramolecular Assembly ◽  
Water Interface ◽  
Ether Bond ◽  
Air Water Interface ◽  
Small Change

How does a small change in the structure of a phospholipid affect its supramolecular assembly?

Ordered supramolecular assembly of bis[3,4,1 2,13,21,22,30, 31-octa(dodecylthio)-2,3-naphthalocyaninato] erbium at the air/water interface

2001 ◽  
Vol 44 (6) ◽  
pp. 650-656 ◽  
Author(s):  
Yanli Chen ◽  
Hongguo Liu ◽  
Na Pan ◽  
Xianxi Zhang ◽  
Wei Liu ◽  
...  
Keyword(s):  
Supramolecular Assembly ◽  
Water Interface ◽  
Air Water Interface

scholarly journals Influence of a Single Ether Bond on Assembly, Orientation, and Miscibility of Phosphocholine Lipids at the Air-Water Interface

2020 ◽  
Author(s):  
Matthias Hoffmann ◽  
Simon Drescher ◽  
Christian Schwieger ◽  
Dariush Hinderberger
Keyword(s):  
Supramolecular Assembly ◽  
Phase Behaviour ◽  
Epifluorescence Microscopy ◽  
Ether Linkage ◽  
Infrared Reflection Absorption Spectroscopy ◽  
Infrared Reflection ◽  
Air Water Interface ◽  
Lipid Chain ◽  
Ester Linkage ◽  
Small Change

How does a small change in the structure of a phospholipid affect its supramolecular assembly? In aqueous suspensions, the substitution of one ester linkage in DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) by an ether linkage alters its phase behaviour completely. To unravel the effect of replacing a phospholipid’s ester linkage by an ether linkage in lipid monolayers, we characterized pure monolayers of the model lipid DPPC and its sn-2 ether analogue PHPC (1-palmitoyl-2-O-hexadecyl-sn-glycero-3-phosphocholine) as well as mixtures of both by measurements of surface pressure – molecular area (π–A_mol) isotherms. In addition, we used infrared reflection absorption spectroscopy (IRRAS) and epifluorescence microscopy to study lipid condensation, lipid chain orientation, headgroup hydration, and lipid miscibility in all samples.

scholarly journals Influence of a Single Ether Bond on Assembly, Orientation, and Miscibility of Phosphocholine Lipids at the Air-Water Interface

2020 ◽  
Author(s):  
Matthias Hoffmann ◽  
Simon Drescher ◽  
Christian Schwieger ◽  
Dariush Hinderberger
Keyword(s):  
Supramolecular Assembly ◽  
Phase Behaviour ◽  
Epifluorescence Microscopy ◽  
Ether Linkage ◽  
Infrared Reflection Absorption Spectroscopy ◽  
Infrared Reflection ◽  
Air Water Interface ◽  
Lipid Chain ◽  
Ester Linkage ◽  
Small Change

How does a small change in the structure of a phospholipid affect its supramolecular assembly? In aqueous suspensions, the substitution of one ester linkage in DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) by an ether linkage alters its phase behaviour completely. To unravel the effect of replacing a phospholipid’s ester linkage by an ether linkage in lipid monolayers, we characterized pure monolayers of the model lipid DPPC and its sn-2 ether analogue PHPC (1-palmitoyl-2-O-hexadecyl-sn-glycero-3-phosphocholine) as well as mixtures of both by measurements of surface pressure – molecular area (π–A_mol) isotherms. In addition, we used infrared reflection absorption spectroscopy (IRRAS) and epifluorescence microscopy to study lipid condensation, lipid chain orientation, headgroup hydration, and lipid miscibility in all samples.

scholarly journals Influence of a Single Ether Bond on Assembly, Orientation, and Miscibility of Phosphocholine Lipids at the Air-Water Interface

2020 ◽  
Author(s):  
Matthias Hoffmann ◽  
Simon Drescher ◽  
Christian Schwieger ◽  
Dariush Hinderberger
Keyword(s):  
Supramolecular Assembly ◽  
Phase Behaviour ◽  
Epifluorescence Microscopy ◽  
Ether Linkage ◽  
Infrared Reflection Absorption Spectroscopy ◽  
Infrared Reflection ◽  
Air Water Interface ◽  
Lipid Chain ◽  
Ester Linkage ◽  
Small Change

How does a small change in the structure of a phospholipid affect its supramolecular assembly? In aqueous suspensions, the substitution of one ester linkage in DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) by an ether linkage alters its phase behaviour completely. To unravel the effect of replacing a phospholipid’s ester linkage by an ether linkage in lipid monolayers, we characterized pure monolayers of the model lipid DPPC and its sn-2 ether analogue PHPC (1-palmitoyl-2-O-hexadecyl-sn-glycero-3-phosphocholine) as well as mixtures of both by measurements of surface pressure – molecular area (π–A_mol) isotherms. In addition, we used infrared reflection absorption spectroscopy (IRRAS) and epifluorescence microscopy to study lipid condensation, lipid chain orientation, headgroup hydration, and lipid miscibility in all samples.

Organized Assembly and Morphologies of Aromatic Schiff Base Compounds in Organized Molecular Films

2013 ◽  
Vol 706-708 ◽  
pp. 176-179
Author(s):  
Yong Xiao
Keyword(s):  
Phase Transition ◽  
Schiff Base ◽  
Molecular Conformation ◽  
Pure Water ◽  
Three Dimensional ◽  
Supramolecular Assembly ◽  
Multilayer Films ◽  
Water Interface ◽  
Air Water Interface ◽  
Langmuir Blodgett Films

A novel aromatic Schiff base ligand has been designed and its interfacial phase behavior as well as the in situ coordination with Cu(II) ion was investigated. A series of measurement methods, such as surface pressure-area isotherms and atomic force microscopy measurement, have been used to characterize the deposited monolayer and multilayer films. It has been found that both the ligand and the corresponding complex formed stable monolayer or multilayer films at the air/water interface, which could be subsequently transferred onto solid substrates to construct Langmuir-Blodgett films. It was interesting to note that there existed a novel phase transition when the ligand was spread on pure water surface. During this phase transition, the two dimensional flat film changed into a three dimensional fiber-like nano-architectures. However, upon coordinating with Cu(II) ions, only flat films were obtained. Such distinct difference was suggested to be mainly due to the change of molecular conformation and/or the hydrophobicity in the process of supramolecular assembly at the air/water interface.

Sampling and analysis of the air-water interface with SEM and EDS of microlayers

1989 ◽  
Vol 47 ◽  
pp. 1004-1005
Author(s):  
Randall W. Smith ◽  
John Dash
Keyword(s):  
Heavy Metals ◽  
Surface Microlayer ◽  
Surface Films ◽  
Water Interface ◽  
Physical Processes ◽  
Infrared Spectroscopic Analysis ◽  
Eds Analysis ◽  
Air Water Interface ◽  
Significant Area ◽  
Bulk Chemical

The structure of the air-water interface forms a boundary layer that involves biological ,chemical geological and physical processes in its formation. Freshwater and sea surface microlayers form at the air-water interface and include a diverse assemblage of organic matter, detritus, microorganisms, plankton and heavy metals. The sampling of microlayers and the examination of components is presently a significant area of study because of the input of anthropogenic materials and their accumulation at the air-water interface. The neustonic organisms present in this environment may be sensitive to the toxic components of these inputs. Hardy reports that over 20 different methods have been developed for sampling of microlayers, primarily for bulk chemical analysis. We report here the examination of microlayer films for the documentation of structure and composition.Baier and Gucinski reported the use of Langmuir-Blogett films obtained on germanium prisms for infrared spectroscopic analysis (IR-ATR) of components. The sampling of microlayers has been done by collecting fi1ms on glass plates and teflon drums, We found that microlayers could be collected on 11 mm glass cover slips by pulling a Langmuir-Blogett film from a surface microlayer. Comparative collections were made on methylcel1ulose filter pads. The films could be air-dried or preserved in Lugol's Iodine Several slicks or surface films were sampled in September, 1987 in Chesapeake Bay, Maryland and in August, 1988 in Sequim Bay, Washington, For glass coverslips the films were air-dried, mounted on SEM pegs, ringed with colloidal silver, and sputter coated with Au-Pd, The Langmuir-Blogett film technique maintained the structure of the microlayer intact for examination, SEM observation and EDS analysis were then used to determine organisms and relative concentrations of heavy metals, using a Link AN 10000 EDS system with an ISI SS40 SEM unit. Typical heavy microlayer films are shown in Figure 3.

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