Aqueous Wittig reaction-mediated fast fluorogenic identification and single-base resolution analysis of 5-formylcytosine in DNA

2020 ◽  
Vol 56 (81) ◽  
pp. 12158-12161
Author(s):  
Qian Zhou ◽  
Kun Li ◽  
Kang-Kang Yu ◽  
Na Li ◽  
Lei Shi ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Wittig Reaction ◽  
Single Base ◽  
Mild Conditions ◽  
Resolution Analysis ◽  
Single Base Resolution ◽  
Wittig Reactions

Aqueous Wittig reactions in nucleic acids under mild conditions for fluorogenic identification of 5fC and single-base resolution sequencing were studied.

scholarly journals Gene specific-loci quantitative and single-base resolution analysis of 5-formylcytosine by compound-mediated polymerase chain reaction

2018 ◽  
Vol 9 (15) ◽  
pp. 3723-3728 ◽  
Author(s):  
Yafen Wang ◽  
Chaoxing Liu ◽  
Xiong Zhang ◽  
Wei Yang ◽  
Fan Wu ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Demethylation ◽  
Chain Reaction ◽  
Single Base ◽  
Active Dna Demethylation ◽  
Key Players ◽  
Resolution Analysis ◽  
Single Base Resolution ◽  
Polymerase Chain

5-Formylcytosine (5fC) is known as one of the key players in the process of active DNA demethylation and displays essential epigenetic functions in mammals.

Quantification and Single-Base Resolution Analysis of N1-Methyladenosine in mRNA by Ligation-Assisted Differentiation

2020 ◽  
Vol 92 (3) ◽  
pp. 2612-2619 ◽  
Author(s):  
Jiang-Hui Ding ◽  
Cheng-Jie Ma ◽  
Meng-Yuan Chen ◽  
Bei Chen ◽  
Bi-Feng Yuan ◽  
...  
Keyword(s):  
Single Base ◽  
Resolution Analysis ◽  
Single Base Resolution

scholarly journals Bisulfite-free, single base-resolution analysis of 5-hydroxymethylcytosine in genomic DNA by chemical-mediated mismatch

2019 ◽  
Vol 10 (2) ◽  
pp. 447-452 ◽  
Author(s):  
Yafen Wang ◽  
Xiong Zhang ◽  
Fan Wu ◽  
Zonggui Chen ◽  
Xiang Zhou
Keyword(s):  
Genomic Dna ◽  
Dna Demethylation ◽  
Nuclear Reprogramming ◽  
Single Base ◽  
Active Dna Demethylation ◽  
Resolution Analysis ◽  
Single Base Resolution

5-Hydroxymethylcytosine (5hmC) is known as one of the vital players in nuclear reprogramming and the process of active DNA demethylation.

scholarly journals Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics

Cell Reports ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. 674-683 ◽  
Author(s):  
Francesco Neri ◽  
Danny Incarnato ◽  
Anna Krepelova ◽  
Stefania Rapelli ◽  
Francesca Anselmi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Single Base ◽  
Promoter Dna Methylation ◽  
Resolution Analysis ◽  
Single Base Resolution ◽  
Promoter Dna

Xeno nucleic acid probes mediated methylation-specific PCR for single-base resolution analysis of N6-methyladenosine in RNAs

The Analyst ◽  
2021 ◽  
Author(s):  
Qinli Pu ◽  
Hongyan Yu ◽  
Xi Zhou ◽  
Junjie Li ◽  
Yu-Jun Yang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gene Locus ◽  
Cost Effective ◽  
Specific Gene ◽  
Rna Modifications ◽  
Nucleic Acid Probes ◽  
Single Base ◽  
Specific Pcr ◽  
Resolution Analysis ◽  
Single Base Resolution

Reliable and cost-effective quantification of RNA modifications at a specific gene locus is essential to elucidate the pathogenic mechanism encoded by RNA epigenetics. Current methods to quantify N6-methyladenosine (m6A) at...

scholarly journals Fluorogenic labeling and single-base resolution analysis of 5-formylcytosine in DNA

2017 ◽  
Vol 8 (11) ◽  
pp. 7443-7447 ◽  
Author(s):  
Chaoxing Liu ◽  
Yafen Wang ◽  
Wei Yang ◽  
Fan Wu ◽  
Weiwu Zeng ◽  
...  
Keyword(s):  
Intramolecular Cyclization ◽  
Single Base ◽  
Resolution Analysis ◽  
Single Base Resolution

Simultaneous fluorogenic switch-on detection and single-base resolution analysis of 5fC through yielding an intramolecular cyclization nucleobase has been presented.

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