Inducing mesenchymal stem cell attachment on non-cell adhesive hydrogels through click chemistry

2020 ◽  
Vol 56 (55) ◽  
pp. 7661-7664
Author(s):  
Alessia Battigelli ◽  
Bethany Almeida ◽  
Shashank Shukla ◽  
Alicia D. Rocha ◽  
Anita Shukla
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Click Chemistry ◽  
Cell Attachment ◽  
Cell Adhesive ◽  
Adhesive Materials

Strain-promoted bio-orthogonal click chemistry is used to initiate cell attachment on non-cell adhesive materials.

scholarly journals Transfer stamping of human mesenchymal stem cell patches using thermally expandable hydrogels with tunable cell-adhesive properties

Biomaterials ◽  
2015 ◽  
Vol 54 ◽  
pp. 44-54 ◽  
Author(s):  
Indong Jun ◽  
Yu Bin Lee ◽  
Yu Suk Choi ◽  
Adam J. Engler ◽  
Hansoo Park ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cell ◽  
Adhesive Properties ◽  
Cell Adhesive

scholarly journals Extracellular matrix derived peptides and mesenchymal stem cell motility

2021 ◽  
Author(s):  
◽  
Sandi Grainne Dempsey
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Motility ◽  
Cell Attachment ◽  
Mass Spectrometry Analysis ◽  
And Migration ◽  
Proliferation And Migration

<p>Biomaterials derived from decellularised extracellular matrices have shown promise as tools in tissue regeneration and wound healing. Such materials display biocompatibility as well as inherent bioactivity, promoting constructive remodelling in healing tissues. In this study, the bioactivity of ovine forestomach matrix (a decellularised extracellular matrix biomaterial) is assessed based on its ability to affect the proliferation and migration of wound healing cells.  This material supported cell attachment and proliferation, but did not allow cell infiltration in vitro. Enzymatic digestion of the material rendered soluble components that were able to induce proliferation and migration of some cell types. Cell-mediated processing of the material generated a protein or proteins with chemotactic activity for mesenchymal stem cells in vitro. Mass spectrometry analysis indicated the bioactive component consisted of the proteoglycan decorin, or fragments thereof. Decorin has not previously been shown to induce mesenchymal stem cell motility, and these findings may add to what is known about decorin and its role in constructive remodelling. Furthermore, this cell-mediated approach for ECM breakdown could lead to the discovery of other bioactive peptides involved in ECM remodelling and wound healing.</p>

Screening rat mesenchymal stem cell attachment and differentiation on surface chemistries using plasma polymer gradients

2015 ◽  
Vol 11 ◽  
pp. 58-67 ◽  
Author(s):  
Peng-Yuan Wang ◽  
Lauren R. Clements ◽  
Helmut Thissen ◽  
Wei-Bor Tsai ◽  
Nicolas H. Voelcker
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Attachment ◽  
Plasma Polymer ◽  
Surface Chemistries

Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

2010 ◽  
Vol 208 (6) ◽  
pp. 1440-1445 ◽  
Author(s):  
Lauren R. Clements ◽  
Peng-Yuan Wang ◽  
Frances Harding ◽  
Wei-Bor Tsai ◽  
Helmut Thissen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Porous Silicon ◽  
Mesenchymal Stem Cell ◽  
Cell Attachment ◽  
Density Gradients

scholarly journals ID: 1060 Organic-inorganic Biomaterials promote mesenchymal stem cell proliferation and differentiation for long-term cultivation

2017 ◽  
Vol 4 (S) ◽  
pp. 141
Author(s):  
Umul Hanim Yusoff ◽  
Shamsi Ebrahimi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Material Culture ◽  
Cell Attachment ◽  
Adipogenic Differentiation ◽  
Cell Phenotype ◽  
Cover Slip

Background: A nifty propagating of mesenchymal stem cell (MSCs) diligence has germinated all over the world by innovative investigators. However, the clinical and basic research applications of MSC requires novel finding biomaterials interfacial interaction especially in sustainable the morphology, physiology, multipotent and phenotypically in long-term cultivation. A prominent of biomaterials benefit to MSCs culture has triggered the multitudinous field especially in regenerative medicine. In order to hinder the deprivation of MSCs in purity and potency, the alternative cell-substrate materials of MSCs culture is essentially to be discovered. This has instigated the idea to encountered the method of screening libraries organic and inorganics biomaterials in bio-adhesively, free ethically, and sustainability to support the morphologically, physiologically, multi-potent and phenotypically of substrates coating cover slip.  Methods: Libraries of inorganic biomaterials substrates have been collected from co-researcher to conduct the initial screening phase of 100 myriad fabrications of substrates whereas enumerated as a Graphene Oxide (GO), Hydroxyapatite (HAp), and Bioactive Glass (BAG) coated cover slip and discs also several organic biomaterials. Wharton’s Jelly derived Mesenchymal Stem Cells (WJMSCs) and Denuded Amnion Mesenchymal Stem Cells (AMMSCs) have been seeded on each substrate in the 48-well plate. Top four leading substrates have been selected for further cultivation until up to 5 passage (>P5) for long term screening known as scaling up phase. Several parameters such as cell attachment, cell viability, kinetic growth, cell-materials osteogenic and adipogenic differentiation and cell phenotype have been analyzed. Top one cell-material culture will go forward to further long-term cultivation up to Passage 10(>P10).  Results: Morphologically and phenotypically demonstrated that GOy1WJMSC showed the significance result among others.

scholarly journals Extracellular matrix derived peptides and mesenchymal stem cell motility

2021 ◽  
Author(s):  
◽  
Sandi Grainne Dempsey
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Motility ◽  
Cell Attachment ◽  
Mass Spectrometry Analysis ◽  
And Migration ◽  
Proliferation And Migration

<p>Biomaterials derived from decellularised extracellular matrices have shown promise as tools in tissue regeneration and wound healing. Such materials display biocompatibility as well as inherent bioactivity, promoting constructive remodelling in healing tissues. In this study, the bioactivity of ovine forestomach matrix (a decellularised extracellular matrix biomaterial) is assessed based on its ability to affect the proliferation and migration of wound healing cells.  This material supported cell attachment and proliferation, but did not allow cell infiltration in vitro. Enzymatic digestion of the material rendered soluble components that were able to induce proliferation and migration of some cell types. Cell-mediated processing of the material generated a protein or proteins with chemotactic activity for mesenchymal stem cells in vitro. Mass spectrometry analysis indicated the bioactive component consisted of the proteoglycan decorin, or fragments thereof. Decorin has not previously been shown to induce mesenchymal stem cell motility, and these findings may add to what is known about decorin and its role in constructive remodelling. Furthermore, this cell-mediated approach for ECM breakdown could lead to the discovery of other bioactive peptides involved in ECM remodelling and wound healing.</p>

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