A two-photon fluorescent probe for sensitive detection and imaging of γ-glutamyl transpeptidase

2020 ◽  
Vol 56 (74) ◽  
pp. 10902-10905
Author(s):  
Ruijin Huo ◽  
Xiuli Zheng ◽  
Weimin Liu ◽  
Liping Zhang ◽  
Jiasheng Wu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Sensitive Detection ◽  
Ovarian Cancer Cells ◽  
Normal Cells ◽  
Two Photon ◽  
Glutamyl Transpeptidase

A GGT-activated two-photon fluorescent probe was developed for distinguishing ovarian cancer cells from normal cells.

Selective tracking of ovarian-cancer-specific γ-glutamyltranspeptidase using a ratiometric two-photon fluorescent probe

2018 ◽  
Vol 6 (45) ◽  
pp. 7439-7443 ◽  
Author(s):  
Ben Shi ◽  
Zhenyu Zhang ◽  
Qingqing Jin ◽  
Zhiqiang Wang ◽  
Jie Tang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Enzymatic Activity ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Cancer Diagnosis ◽  
Reliable Method ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Two Photon ◽  
Real Time Tracking

Real-time tracking of GGT enzymatic activity in human ovarian cancer cells is a reliable method for accurate prediction of cancer diagnosis and management.

scholarly journals Ovarian cancer cells, not normal cells, are damaged by Mirk/Dyrk1B kinase inhibition

2012 ◽  
Vol 132 (10) ◽  
pp. 2258-2269 ◽  
Author(s):  
Jing Hu ◽  
Holly Deng ◽  
Eileen A. Friedman
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Normal Cells ◽  
Kinase Inhibition

A fluorescent probe for imaging hydrogen peroxide in ovarian cancer cells

2018 ◽  
Vol 155 ◽  
pp. 143-149 ◽  
Author(s):  
Peng Zhao ◽  
Kunyan Wang ◽  
Xiaomin Zhu ◽  
Yuren Zhou ◽  
Junchen Wu
Keyword(s):  
Ovarian Cancer ◽  
Hydrogen Peroxide ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Ovarian Cancer Cells

scholarly journals Nuclear Lamin A/C Expression Is a Key Determinant of Paclitaxel Sensitivity

2021 ◽  
Author(s):  
Elizabeth R. Smith ◽  
Justin Leal ◽  
Celina Amaya ◽  
Bing Li ◽  
Xiang-Xi Xu
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Nuclear Lamina ◽  
Current Treatment ◽  
Nuclear Shape ◽  
Ovarian Cancer Cells ◽  
Lamin A ◽  
Cancer Therapies ◽  
Normal Cells ◽  
Nuclear Lamin

Paclitaxel is a key member of the Taxane (Taxol/paclitaxel, docetaxel/taxotere) family of successful drugs used in the current treatment of several solid tumors, including ovarian cancer. The molecular target of Taxol/paclitaxel has been identified as tubulin, and paclitaxel binding alters the dynamics and thus stabilizes microtubule bundles. Traditionally, the anti-cancer mechanism of paclitaxel has been thought to originate from its interfering with the role of microtubules in mitosis, resulting in mitotic arrest and subsequent apoptosis. However, recent evidence suggests that paclitaxel operates in cancer therapies via an as-yet-undefined mechanism rather than as a mitotic inhibitor. We found that paclitaxel caused a striking break up of nuclei (referred to as multimicronucleation) in malignant ovarian cancer cells but not in normal cells, and susceptibility to undergo nuclear fragmentation and cell death correlated with a reduction in nuclear lamina proteins, Lamin A/C. Lamin A/C proteins are commonly lost, reduced, or heterogeneously expressed in ovarian cancer, accounting for the aberration of nuclear shape in malignant cells. Mouse ovarian epithelial cells isolated from Lamin A/C null mice were highly sensitive to paclitaxel and underwent nuclear breakage, compared to control wildtype cells. Forced over-expression of Lamin A/C led to resistance to paclitaxel-induced nuclear breakage in cancer cells. Additionally, paclitaxel-induced multimicronucleation occurred independently of cell division that was achieved either by the withdrawal of serum or addition of mitotic inhibitors. These results provide a new understanding for the mitotic-independent mechanism for paclitaxel killing of cancer cells, where paclitaxel induces nuclear breakage in malignant cancer cells that have a malleable nucleus, but not in normal cells that have a stiffer nuclear envelope. As such, we identify that reduced nuclear Lamin A/C protein levels correlate with nuclear shape deformation and is a key determinant of paclitaxel sensitivity of cancer cells.

Cytotoxic effects of disulfiram and synergism with cisplatin on ovarian cancer cells in vitro

2018 ◽  
Author(s):  
F Guo ◽  
Z Yang ◽  
J Xu ◽  
J Sehouli ◽  
AE Albers ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Cytotoxic Effects
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