A polynuclear Cu(ii) complex for real time monitoring of mitochondrial cytochrome C release during cellular apoptosis

2020 ◽  
Vol 56 (48) ◽  
pp. 6563-6566
Author(s):  
Somnath Khanra ◽  
Sabyasachi Ta ◽  
Ankush Paladhi ◽  
Milan Ghosh ◽  
Subhasis Ghosh ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Single Crystal ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Live Cells ◽  
Mitochondrial Cytochrome ◽  
Cytochrome C Release ◽  
X Ray ◽  
Cellular Apoptosis ◽  
Intracellular Translocation

Single crystal X-ray structurally characterized poly-nuclear Cu(ii) complex (M1) selectively and spatially interacts with Cytochrome C (Cyt C) to allow fluorescence imaging of intracellular translocation events in live cells during apoptotic process.

Platycodon grandiflorum Induces Apoptosis in SKOV3 Human Ovarian Cancer Cells Through Mitochondrial-Dependent Pathway

2010 ◽  
Vol 38 (02) ◽  
pp. 373-386 ◽  
Author(s):  
Qin Hu ◽  
Ruile Pan ◽  
Liwei Wang ◽  
Bo Peng ◽  
Jingtian Tang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cytochrome C ◽  
Cancer Cells ◽  
Caspase 3 ◽  
Ovarian Cancer Cells ◽  
Mitochondrial Cytochrome ◽  
Cytochrome C Release ◽  
Apoptotic Pathway ◽  
Platycodon Grandiflorum ◽  
Mitochondrial Cytochrome C

Platycodon grandiflorum (Jacq.) A. DC., a Chinese food and medicine, has been used as expectorant traditionally. The present study aimed to investigate the effect of Platycodon grandiflorum extract (PGE) on SKOV3 ovarian cancer cells. 3-(4,5- dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay was used to monitor cell numbers, Annexin-V/propidium iodide (PI) staining, RT-PCR and Western blot were used to examine cell apoptosis, caspases activation. Bcl-2 and Bax expressions and mitochondrial cytochrome c release. Our result showed that PGE-induced apoptosis was associated with activation of caspase-3, -8 and -9, down-regulation of Bcl-2, up-regulation of Bax and release of mitochondrial cytochrome c to cytosol. The data indicate that PGE may have anti-tumor effect mainly via caspase-3 and caspase-9 dependent apoptotic pathway.

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