Engineering of a Au-sensor to develop a Hg-specific, sensitive and robust whole-cell biosensor for on-site water monitoring

2020 ◽  
Vol 56 (48) ◽  
pp. 6590-6593
Author(s):  
Julián I. Mendoza ◽  
Fernando C. Soncini ◽  
Susana K. Checa
Keyword(s):  
Analytical Tool ◽  
Water Monitoring ◽  
Whole Cell ◽  
Sensor Protein ◽  
Site Water ◽  
Sensitive Sensor ◽  
Whole Cell Biosensor ◽  
Cell Biosensor

A Hg-specific and sensitive sensor protein was designed and integrated into an optimized whole-cell biosensor platform. Quantitative and semiquantitative approaches validate the application of this analytical tool for on-site Hg monitoring.

scholarly journals Influence of the luxR Regulatory Gene Dosage and Expression Level on the Sensitivity of the Whole-Cell Biosensor to Acyl-Homoserine Lactone

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 166
Author(s):  
Sergey Bazhenov ◽  
Uliana Novoyatlova ◽  
Ekaterina Scheglova ◽  
Vadim Fomin ◽  
Svetlana Khrulnova ◽  
...  
Keyword(s):  
Gene Dosage ◽  
Regulatory Gene ◽  
Homoserine Lactone ◽  
Threshold Concentration ◽  
Expression Level ◽  
Whole Cell ◽  
Lux Biosensors ◽  
Order Of Magnitude ◽  
Whole Cell Biosensor ◽  
Cell Biosensor

Aliivibrio fischeri LuxR and Aliivibrio logei LuxR1 and LuxR2 regulatory proteins are quorum sensing transcriptional (QS) activators, inducing promoters of luxICDABEG genes in the presence of an autoinducer (3-oxo-hexanoyl-l-homoserine lactone). In the Aliivibrio cells, luxR genes are regulated by HNS, CRP, LitR, etc. Here we investigated the role of the luxR expression level in LuxI/R QS system functionality and improved the whole-cell biosensor for autoinducer detection. Escherichia coli-based bacterial lux-biosensors were used, in which Photorhabdus luminescensluxCDABE genes were controlled by LuxR-dependent promoters and luxR, luxR1, or luxR2 regulatory genes. We varied either the dosage of the regulatory gene in the cells using additional plasmids, or the level of the regulatory gene expression using the lactose operon promoter. It was shown that an increase in expression level, as well as dosage of the regulatory gene in biosensor cells, leads to an increase in sensitivity (the threshold concentration of AI is reduced by one order of magnitude) and to a two to threefold reduction in response time. The best parameters were obtained for a biosensor with an increased dosage of luxRA. fischeri (sensitivity to 3-oxo-hexanoyl-l-homoserine lactone reached 30–100 pM).

scholarly journals Detection of Bioavailable Cadmium by Double-Color Fluorescence Based on a Dual-Sensing Bioreporter System

2021 ◽  
Vol 12 ◽  
Author(s):  
Chang-ye Hui ◽  
Yan Guo ◽  
Jian Wu ◽  
Lisa Liu ◽  
Xue-qin Yang ◽  
...  
Keyword(s):  
Anthropogenic Activities ◽  
Simultaneous Detection ◽  
Environmental Water ◽  
High Concentration ◽  
Whole Cell ◽  
Specificity And Sensitivity ◽  
Improved Performance ◽  
Whole Cell Biosensor ◽  
Cell Biosensor ◽  
Dual Sensing

Cadmium (Cd) is carcinogenic to humans and can accumulate in the liver, kidneys, and bones. There is widespread presence of cadmium in the environment as a consequence of anthropogenic activities. It is important to detect cadmium in the environment to prevent further exposure to humans. Previous whole-cell biosensor designs were focused on single-sensing constructs but have had difficulty in distinguishing cadmium from other metal ions such as lead (Pb) and mercury (Hg). We developed a dual-sensing bacterial bioreporter system to detect bioavailable cadmium by employing CadC and CadR as separate metal sensory elements and eGFP and mCherry as fluorescent reporters in one genetic construct. The capability of this dual-sensing biosensor was proved to simultaneously detect bioavailable cadmium and its toxic effects using two sets of sensing systems while still maintaining similar specificity and sensitivity of respective signal-sensing biosensors. The productions of double-color fluorescence were directly proportional to the exposure concentration of cadmium, thereby serving as an effective quantitative biosensor to detect bioavailable cadmium. This novel dual-sensing biosensor was then validated to respond to Cd(II) spiked in environmental water samples. This is the first report of the development of a novel dual-sensing, whole-cell biosensor for simultaneous detection of bioavailable cadmium. The application of two biosensing modules provides versatile biosensing signals and improved performance that can make a significant impact on monitoring high concentration of bioavailable Cd(II) in environmental water to reduce human exposure to the harmful effects of cadmium.

scholarly journals Development of a pigment-based whole-cell biosensor for the analysis of environmental copper

RSC Advances ◽  
2017 ◽  
Vol 7 (47) ◽  
pp. 29302-29305 ◽  
Author(s):  
Pei-Hsuan Chen ◽  
Chieh Lin ◽  
Kai-Hong Guo ◽  
Yi-Chun Yeh
Keyword(s):  
Whole Cell ◽  
Whole Cell Biosensor ◽  
Cell Biosensor

A engineered whole-cell biosensor is developed to generate output signals for the environmental copper analysis.

Quantification of bioavailable chlortetracycline in pig feces using a bacterial whole-cell biosensor

2002 ◽  
Vol 87 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Lars Hestbjerg Hansen ◽  
Frank Aarestrup ◽  
Søren Johannes Sørensen
Keyword(s):  
Whole Cell ◽  
Whole Cell Biosensor ◽  
Cell Biosensor

scholarly journals Whole-cell biosensor of cellobiose and application to wood decay detection

2016 ◽  
Vol 239 ◽  
pp. 39-46 ◽  
Author(s):  
Maxime Toussaint ◽  
Cyril Bontemps ◽  
Arnaud Besserer ◽  
Laurence Hotel ◽  
Philippe Gérardin ◽  
...  
Keyword(s):  
Wood Decay ◽  
Whole Cell ◽  
Whole Cell Biosensor ◽  
Cell Biosensor

Detection of chlorinated and brominated hydrocarbons by an ion sensitive whole cell biosensor

1996 ◽  
Vol 11 (12) ◽  
pp. 1215-1219 ◽  
Author(s):  
J. Peter ◽  
W. Hutter ◽  
W. Stöllnberger ◽  
W. Hampel
Keyword(s):  
Whole Cell ◽  
Whole Cell Biosensor ◽  
Cell Biosensor
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