Biomimetic nanofibrous hybrid hydrogel membranes with sustained growth factor release for guided bone regeneration

2021 ◽  
Vol 9 (4) ◽  
pp. 1256-1271
Author(s):  
Qingyue Yuan ◽  
Lunhao Li ◽  
Yiyu Peng ◽  
Ai Zhuang ◽  
Wei Wei ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Growth Factor ◽  
Sustained Release ◽  
Guided Bone Regeneration ◽  
Hybrid Hydrogel ◽  
Hydrogel Membranes ◽  
Prolonged Effect ◽  
Growth Factor Release ◽  
Factor Release

A biomimetic nanofibrous membrane can immobilize growth factors or agents to obtain sustained release and prolonged effect in tissue engineering.

scholarly journals P5: EFFECT OF VITAMIN C AND COLD ON GROWTH FACTOR RELEASE FROM PLATELET-RICH PLASMA

2021 ◽  
Vol 108 (Supplement_1) ◽  
Author(s):  
T Tomouk ◽  
S Talaat ◽  
O Smith ◽  
A Mosahebi
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Vitamin C ◽  
Sustained Release ◽  
Platelet Rich Plasma ◽  
Growth Factor Release ◽  
Cold Pack ◽  
B Vitamin ◽  
Factor Release ◽  
Vit C

Abstract Introduction Platelet-rich plasma (PRP) has gathered great momentum in surgery. The main outcome of the study was to assess whether there was a difference in growth factor release from PRP with addition of vitamin C, cooling, or both vitamin C and cooling. Method Blood samples from 10 volunteers were centrifuged to produce PRP. Activated autologous thrombin was produced from platelet-poor plasma (PPP), which was added to PRP to activate it. PRP from each participant was then divided by activation method into three groups: A) cold pack only; B) vitamin C only; C) cold pack/vitamin C. Growth factor quantification was performed for human VEGF, IGF-1 and PDGF-AB using enzyme-linked immunosorbent assays. Result At 24 hours, VEGF concentration in the cold group was significantly higher cf. the vitamin C group (p=0.02) and the cold/vit C group (p<0.01), whereas IGF concentration in the cold group was significantly higher cf. the vitamin C group (p=0.02) but not the cold/vit C group. At 1 hour and 24 hours, PDGF concentration in the cold group was significantly higher cf. both the vitamin C group (p<0.01) and the cold/vit C group (p=0.02 and p<0.0001 respectively). Conclusion Cooling lead to increased growth factor release at 24 hours, indicating that cooling may lead to sustained release of growth factors. There was a trend for PRP treated with vitamin C to remain in a gel-like consistency rather than clot. This may prove useful in clinical practice where currently activated PRP is constrained to topical application only due to its consistency. Take-home message Cooling may lead to sustained release of growth factors from platelet-rich plasma, whereas the addition of vitamin C may provide opportunity for injectable activated PRP.

Effects of Aspirin on Growth Factor Release From Freshly Isolated Leukocyte-Rich Platelet-Rich Plasma in Healthy Men: A Prospective Fixed-Sequence Controlled Laboratory Study

2019 ◽  
Vol 47 (5) ◽  
pp. 1223-1229 ◽  
Author(s):  
Prathap Jayaram ◽  
Peter Yeh ◽  
Shiv J. Patel ◽  
Racel Cela ◽  
Theodore B. Shybut ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Whole Blood ◽  
Low Dose ◽  
Platelet Rich Plasma ◽  
Healthy Men ◽  
Growth Factor Release ◽  
Tgf Β1 ◽  
Cox 1 ◽  
Factor Release

Background: The benefits of platelet-rich plasma (PRP) are believed to be in part dependent on growth factor release after platelet activation. Platelet activation is complex and involves multiple mechanisms. One important mechanism is driven by cyclooxygenase 1 (COX-1)–mediated conversion of arachidonic acid (AA) to precursor prostaglandins that then mediate proinflammatory responses that trigger growth factor release. Acetylsalicylic acid (ASA; also known as aspirin) is known to irreversibly inhibit COX-1, thereby blocking AA-mediated signaling; however, it is unclear whether ASA use alters growth factor release from freshly isolated PRP. Purpose: To assess the effects of low-dose ASA use on activation of growth factor release from freshly isolated human PRP via AA and thrombin (TBN). Study Design: Controlled laboratory study. Methods: Twelve healthy men underwent blood collection and leukocyte-rich PRP (LR-PRP) preparation through a double-spin protocol to obtain baseline whole blood and PRP counts the same day. PRP was aliquoted into 3 groups: nonactivated, AA activated, and TBN activated. Immediately after activation, the concentrations of transforming growth factor β1 (TGF-β1), vascular endothelial growth factor (VEGF), and platelet-derived growth factor AB (PDGF-AB) were measured using enzyme-linked immunosorbent assays (ELISAs). The same 12 participants were then placed on an 81-mg daily dose of oral ASA for 14 days. Repeat characterization of whole blood and PRP analyses was done on day 14, followed by repeat ELISAs of growth factors under the same nonactivated and activated settings as previously stated. Results: Fourteen days of daily ASA had no effect on the number of platelets and leukocytes measured in whole blood and LR-PRP. Compared with nonactivated LR-PRP, AA- and TBN-mediated activation led to significant release of VEGF and PDGF-AB. In contrast, release of TGF-β1 from LR-PRP was observed only with activation by AA, not with TBN. Consistent with its inhibitory role in AA signaling, ASA significantly inhibited AA-mediated release of all 3 growth factors measured in this study. Although ASA had no effect on TBN-mediated release of VEGF and TGF-β1 from LR-PRP, ASA did partially block TBN-mediated release of PDGF-AB, although the mechanism remains unclear. Conclusion: Daily use of low-dose ASA reduces VEGF, PDGF-AB, and TGF-β1 expression in freshly isolated human LR-PRP when activated with AA. Clinical Relevance: Reduction in growth factor release attributed to daily use of low-dose ASA or other COX inhibitors can be mitigated when PRP samples are activated with TBN. Clinical studies are needed to determine whether activation before PRP injection is needed in all applications where ASA is in use and to what extent ASA may inhibit growth factor release in vivo at the site of injury.

Development of a dual growth factor loaded biodegradable hydrogel and its evaluation on osteoblast differentiation in vitro

2011 ◽  
Vol 1312 ◽  
Author(s):  
Deepti Dyondi ◽  
Thomas J Webster ◽  
Rinti Banerjee
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Osteoblast Differentiation ◽  
Chitosan Nanoparticles ◽  
Marker Genes ◽  
Tissue Engineering Scaffolds ◽  
Biodegradable Hydrogel ◽  
Growth Factor Release ◽  
Factor Release

ABSTRACTHydrogels with their tunable properties are attractive candidates for developing tissue engineering scaffolds for various applications (including bone and cartilage). The current work involved studying the synergistic effect of basic fibroblast growth factor (bFGF) and platelet derived growth factor BB (PDGF-BB) entrapped within injectable porous gels for bone regeneration applications. An in situ gelling system was developed using bacterial polysaccharides gellan and xanthan gum by temperature and ionic gelation with Ca+2. After the initial characterization of the hydrogels, a dual growth factor release system was developed wherein growth factors were encapsulated within chitosan nanoparticles embedded in the gels as well as directly within the gel. The hydrogel structure was characterized by SEM and TEM and in vitro growth factor release studies showed a slow release profile in PBS. Further, human fetal osteoblasts were entrapped within the hydrogel and a 21 day osteoblast differentiation study was conducted. An improvement in osteoblast total protein synthesis and collagen content was observed by day 21 compared to control gels without growth factors. Although further evaluation regarding mechanical properties and expression of osteogenic differentiation marker genes will be necessary, the present study suggests that injectable scaffolds can be used for the delivery of multiple growth promoting agents to support osteoblast differentiation.

scholarly journals Effect of double growth factor release on cartilage tissue engineering

2011 ◽  
Vol 7 (2) ◽  
pp. 149-160 ◽  
Author(s):  
Ayşe Burcu Ertan ◽  
Pınar Yılgor ◽  
Banu Bayyurt ◽  
Ayşe Ceren Çalıkoğlu ◽  
Çiğdem Kaspar ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Growth Factor ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Growth Factor Release ◽  
Factor Release
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